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Genomic organization and promoter analysis of a transcriptional repressor gene from Fenneropenaeus chinensis

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Abstract

In this study, we cloned and sequenced genomic sequences from a Fenneropenaeus chinensis transcriptional repressor gene, FcTR. The FcTR gene is 2,671 bp in length and has four exons and three introns. The 873 bp promoter contains several transcription factor binding sites, including a TATA box and a cyclic AMP-responsive element. Promoter deletion analysis using a luciferase reporter gene identified regulatory elements. Challenge with white spot syndrome virus increased expression from the promoter-deletion constructs. These results suggest that FcTR might play an important role in the shrimp immune response.

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Abbreviations

TFs:

Transcription factors

Gfi1:

Growth factor independence 1

WSSV:

White spot syndrome virus

PCR:

Polymerase chain reaction

FCS:

Fetal calf serum

MOI:

Multiplicity of infection

TBP:

TATA box

CRE:

Cyclic AMP-responsive element

CF2-II:

Cell factor 2-II

BR-C Z:

Broad-complex

Kr:

Kruppel

HSF:

Heat-shock factor

AP-1:

Activator protein-1

Hb:

Hunchback

MYBs:

MYB domain

ERFs:

Ethylene response factors

WRKYs:

WRKY domain

bZIPs:

Basic leucine zipper

LITAF:

Lipopolysaccharide-induced tumor necrosis factor-α

ETS:

E26 transformation-specific

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Acknowledgments

This work was financially supported by Natural Science Foundation of Lianyungang (CN1306), The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Project supported by the National Natural Science Foundation of China (31472282), Aquatic three-new projects of Jiangsu (D2014-17-1), Project supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (13KJA240001), and by Jiangsu Key Laboratory of Marine Biotechnology program of China (No. 2012HS002).

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Authors and Affiliations

  1. Ocean and Fisheries College, Huaihai Institute of Technology, Lianyungang, 222005, China

    Xiaofang Lai, Shanrui Shen, Huan Gao & Binlun Yan

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  1. Xiaofang Lai
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  2. Shanrui Shen
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  3. Huan Gao
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  4. Binlun Yan
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Correspondence to Xiaofang Lai.

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Lai, X., Shen, S., Gao, H. et al. Genomic organization and promoter analysis of a transcriptional repressor gene from Fenneropenaeus chinensis . Mol Biol Rep 42, 393–398 (2015). https://doi.org/10.1007/s11033-014-3780-7

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  • DOI: https://doi.org/10.1007/s11033-014-3780-7

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