Skip to main content
SpringerLink
Log in

Overexpression of a glycosyltransferase gene SrUGT74G1 from Stevia improved growth and yield of transgenic Arabidopsis by catechin accumulation

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Steviol glycoside and gibberellin biosynthetic routes are known as divergent branches of a common origin in Stevia. A UDP-glycosyltransferase encoded by SrUGT74G1 catalyses the conversion of steviolbioside into stevioside in Stevia rebaudiana leaves. In the present study, transgenic Arabidopsis thaliana overexpressing SrUGT74G1 cDNA from Stevia were developed to check the probability of stevioside biosynthesis in them. However, stevioside accumulation was not evident in transgenics. Also, the transgenic Arabidopsis showed no change in GA3 content on SrUGT74G1 overexpression. Surprisingly, significant accumulation of catechin was noticed in transgenics. The transgenics showed a considerable increase in shoot length, root length and rosette area. An increase in free radical scavenging activity of transgenics was noticed. Moreover, the seed yield of transgenics was also increased by 6–15 % than control. Additionally, variation in trichome branching pattern on leaf surface of transgenics was observed. The trichome branching pattern was also validated by exogenous catechin exposure (10, 50, 100 ng ml−1) to control plants. Hence, present study reports the probable role of SrUGT74G1 from Stevia in catechin accumulation of transgenic Arabidopsis thaliana. Thus, detailed study in present perspective has revealed the role of Stevia SrUGT74G1 gene in trichome branching pattern, improved vegetative growth, scavenging potential and seed yield by catechin accumulation in transgenic Arabidopsis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Brandle JE, Rosa N (1992) Heritability for yield, leaf: stem ratio and stevioside content estimated from a landrace cultivar of Stevia rebaudiana. Can J Plant Sci 72:1263–1266

    Article  CAS  Google Scholar 

  2. Wanke M, Tudek KS, Swiezewska E (2001) Isoprenoid biosynthesis via 1-deoxy-d-xylulose-5-phosphate/2-C-methyl-d-erythritol-4-phosphate (DOXP/MEP) pathway. Acta Biochim Pol 48:663–672

    CAS  PubMed  Google Scholar 

  3. Brandle JE, Telmer PG (2007) Steviol glycoside biosynthesis. Phytochemistry 68:1855–1863

    Article  CAS  PubMed  Google Scholar 

  4. Totte N, Charon L, Rohmer M, Compernolle F, Baboeuf I, Geuns JMC (2000) Biosynthesis of the diterpenoid steviol, an ent-kaurene derivative from Stevia rebaudiana Bertoni, via the methylerythritol phosphate pathway. Tetrahedron Lett 41:6407–6410

    Article  CAS  Google Scholar 

  5. Richman AS, Gijzen M, Starratt AN, Yang Z, Brandle JE (1999) Diterpene synthesis in Stevia rebaudiana: recruitment and up-regulation of key enzymes from the gibberellin biosynthetic pathway. Plant J 19:411–421

    Article  CAS  PubMed  Google Scholar 

  6. Yadav SK, Guleria P (2012) Steviol glycosides from Stevia: biosynthesis pathway review and their application in foods and medicine. Crit Rev Food Sci 52:988–998

    Article  CAS  Google Scholar 

  7. Humphrey TV, Richman AS, Menassa R, Brandle JE (2006) Spatial organisation of four enzymes from Stevia rebaudiana that are involved in steviol glycoside synthesis. Plant Mol Biol 61:47–62

    Article  CAS  PubMed  Google Scholar 

  8. Bechtold N, Ellis J, Pelletier G (1993) In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C R Acad Sci Paris Life Sci 316:1194–1199

    CAS  Google Scholar 

  9. Singh K, Raizada J, Bhardwaj P, Ghawana S, Rani A, Singh H, Kaul K, Kumar S (2004) 26S rRNA-based internal control gene primer pair for reverse transcription-polymerase chain reaction-based quantitative expression studies in diverse plant species. Anal Biochem 335:330–333

    Article  CAS  PubMed  Google Scholar 

  10. Jaitak V, Singh BB, Kaul VK (2009) An efficient microwave-assisted extraction process of stevioside and rebaudioside-A from Stevia rebaudiana (Bertoni). Phytochem Anal 20:240–245

    Article  CAS  PubMed  Google Scholar 

  11. Hedden P, Phillips AL (2000) Gibberellin metabolism: new insights revealed by the genes. Trends Plant Sci 5:523–530

    Article  CAS  PubMed  Google Scholar 

  12. Kelen M, Demiralay CE, Sen S, Ozkan G (2004) Separation of abscisic acid, indole-3-acetic acid, gibberellic acid in 99 R (Vitis berlandieri × Vitis rupestris) and rose oil (Rosa damascena Mill.) by reversed phase liquid chromatography. Turk J Chem 28:603–610

    CAS  Google Scholar 

  13. Sharma V, Gulati A, Ravindranath SD, Kumar V (2005) A simple and convenient method for analysis of tea biochemicals by reverse phase HPLC. J Food Compos Anal 18:583–594

    Article  CAS  Google Scholar 

  14. Kubasek WL, Shirley BW, McKillop A, Goodman HM, Briggs W, Ausubel FM (1992) Regulation of flavonoid biosynthetic genes in germinating Arabidopsis seedlings. Plant Cell 4:1229–1236

    CAS  PubMed Central  PubMed  Google Scholar 

  15. Joshi R, Poonam Gulati A (2011) Biochemical attributes of tea flowers (Camellia sinensis) at different developmental stages in the Kangra region of India. Sci Hortic 130:266–274

    Article  CAS  Google Scholar 

  16. Alexander MP (1969) Differential staining of aborted and non-aborted pollen. Stain Tech 44:117–122

    CAS  Google Scholar 

  17. Saito K, Yonekura-Sakakibara K, Nakabayashi R, Higashi Y, Yamazaki M, Tohge T, Fernie AR (2013) The flavonoid biosynthetic pathway in Arabidopsis: structural and genetic diversity. Plant Physiol Biochem. doi:10.1016/j.plaphy.2013.02.001

    PubMed  Google Scholar 

  18. Bros W, Heller W, Michel C, Saran M (1990) Flavonoids as antioxidant, determination of radical-scavenging efficiency. Method Enzymol 186:343–355

    Article  Google Scholar 

  19. Vogt T, Jones P (2000) Glycosyltransferases in plant natural product synthesis: characterization of a supergene family. Trends Plant Sci 5:380–386

    Article  CAS  PubMed  Google Scholar 

  20. Dixon RA, Sumner LW (2003) Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis. Plant Physiol 131:878–885

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. Tohge T, Nishiyama Y, Hirai MY, Yano M, Nakajima J, Awazuhara M, Inoue E, Takahashi H, Goodenowe DB, Kitayama M, Noji M, Yamazaki M, Saito K (2005) Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor. Plant J 42:218–235

    Article  CAS  PubMed  Google Scholar 

  22. Yonekura-Sakakibara K, Fukushima A, Nakabayashi R, Hanada K, Matsuda F, Sugawara S, Inoue E, Kuromori T, Ito T, Shinozaki K, Wangwattana B, Yamazaki M, Saito K (2012) Two glycosyltransferases involved in anthocyanin modification delineated by transcriptome independent component analysis in Arabidopsis thaliana. Plant J 69:154–167

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  23. Rice-Evans CA, Miller NJ, Paganga G (1996) Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Bio Med 20:933–956

    Article  CAS  Google Scholar 

  24. Almajano MP, Delgado ME, Gordon MH (2007) Albumin causes a synergistic increase in the antioxidant activity of green tea catechins in oil-in-water emulsions. Food Chem 102:1375–1382

    Article  CAS  Google Scholar 

  25. Prithiviraj B, Perry LG, Badri DV, Vivanco JM (2007) Chemical facilitation and induced pathogen resistance mediated by a root-secreted phytotoxin. New Phytol 173:852–860

    Article  CAS  PubMed  Google Scholar 

  26. Rani A, Vats SK, Sharma M, Kumar S (2011) Catechin promotes growth of Arabidopsis thaliana with concomitant changes in vascular system, photosynthesis and hormone content. Biol Plantarum 55:779–782

    Article  CAS  Google Scholar 

  27. Higginson T, Li SF, Parish RW (2003) AtMYB103 regulates tapetum and trichome development in Arabidopsis thaliana. Plant J 35:177–192

    Article  CAS  PubMed  Google Scholar 

  28. Woo HH, Jeong BR, Koo KB, Choi JW, Hirsch AM, Hawes MC (2007) Modifying expression of closely related UDP-glycosyltransferases from pea and Arabidopsis results in altered root development and function. Physiol Plantarum 130:250–260

    Article  CAS  Google Scholar 

  29. Grubb CD, Zipp BJ, Ludwig-Muller J, Masuno MN, Molinski TF, Abel S (2004) Arabidopsis glucosyltransferase UGT74B1 functions in glucosinolate biosynthesis and auxin homeostasis. Plant J 40:893–908

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors are thankful to the Director, CSIR-IHBT for his continuous encouragement and support. PG is thankful to the Council of Scientific and Industrial Research (CSIR), Government of India for giving a fellowship in the form of SRF.

Author information

Authors and Affiliations

  1. Academy of Scientific and Innovative Research, New Delhi, India

    Praveen Guleria & Sudesh Kumar Yadav

  2. Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176061, HP, India

    Sudesh Kumar Yadav

Authors
  1. Praveen Guleria
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sudesh Kumar Yadav
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sudesh Kumar Yadav.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guleria, P., Yadav, S.K. Overexpression of a glycosyltransferase gene SrUGT74G1 from Stevia improved growth and yield of transgenic Arabidopsis by catechin accumulation. Mol Biol Rep 41, 1741–1752 (2014). https://doi.org/10.1007/s11033-014-3023-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-014-3023-y

Keywords

Search

Navigation