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Effect of luteinizing hormone/choriogonadotropin receptor (LHCGR) gene on chicken reproductive traits

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Abstract

Luteinizing hormone/choriogonadotropin receptor (LHCGR) gene, potentially related to reproductive traits in chickens, was genotyped by using the Pooled DNA Sequencing, PCR–SSCP and Directing Sequencing techniques. 306 Erlang Mountain chickens form one line (SD03, a line that has been selected for egg quality from a local chicken breed in Sichuan province, China) were genotyped in this study. The associations between LHCGR polymorphisms and six reproductive traits [body weight at first egg (BWAFE), weight of first egg, age at first egg (AFE), number of eggs at 300 days of age (EN), body weight at 300 days of age and egg weight at 300 days of age (EWTA)] were estimated using the one-way analysis of variance method. Results showed that SNP +G4058A and SNP +T4099G of the LHCGR gene were significantly associated with BWFE and AFE. Birds with the AG genotype for the +G4058A SNP exhibited shorter AFE (P < 0.05) and greater EN than those of the GG and AA genotypes, suggesting a balancing selection (overdominance); the effect of allele C in SNP +C3021T and allele C in SNP +T4490C on EN and AFE is additive and may reflect the influence of positive selection. These alleles have promise as genetic markers for future marker-assisted selection.

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Acknowledgments

This work was supported by China Agriculture Research System (CARS-41) and The Twelfth Five Years Plan for breeding program in Sichuan—Selective breeding of new breeds and the synthetic strains in laying hens (2011NZ0099-7). We thank Yao Zhang, Xiaoling Zhao and Yan Wang for helping data collection.

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Authors and Affiliations

  1. Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Ya’an, 625014, People’s Republic of China

    Di Yan Li, Long Zhang, Ming Yao Yang, Hua Dong Yin, Ying Li & Qing Zhu

  2. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, 625014, People’s Republic of China

    Huai Liang Xu

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  1. Di Yan Li
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  2. Long Zhang
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  3. Ming Yao Yang
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  7. Qing Zhu
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Correspondence to Ming Yao Yang or Qing Zhu.

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Di Yan Li and Long Zhang contributed equally to this work.

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Li, D.Y., Zhang, L., Yang, M.Y. et al. Effect of luteinizing hormone/choriogonadotropin receptor (LHCGR) gene on chicken reproductive traits. Mol Biol Rep 40, 7111–7116 (2013). https://doi.org/10.1007/s11033-013-2834-6

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  • DOI: https://doi.org/10.1007/s11033-013-2834-6

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