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Different anti-apoptotic effects of normal and asthmatic serum on normal eosinophil apoptosis depending on house dust mite-specific IgE

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Abstract

We investigated the effect of asthmatic serum on constitutive eosinophil apoptosis in normal subjects. Eosinophil apoptosis in normal subjects was inhibited by asthmatic serum but not normal serum. In a detailed analysis based on the presence of house dust mite (HDM) IgE, HDM IgE-positive (+) asthmatic serum was more effective for eosinophil apoptosis than that of HDM IgE-negative (−) asthmatic serum. HDM IgE+ asthmatic serum inhibited both HDM IgE− and HDM IgE+ normal eosinophil apoptosis, and HDM IgE− asthmatic serum suppressed eosinophil apoptosis of HDM IgE+ normal. HDM IgE− normal serum did not inhibit either HDM IgE− or HDM IgE+ normal eosinophil apoptosis, and HDM IgE+ normal serum inhibited HDM IgE+ normal eosinophil apoptosis. The kind of HDM IgE (Dermatophagoides pteronissinus-specific IgE and Dermatophagoides farinae-specific IgE) was not related to the effect of asthmatic serum on eosinophil apoptosis. Extracts of DP and DF, Der p1, and Der p2, were not effective for eosinophil apoptosis. HDM IgE+ asthmatic serum inhibited cleavage of procaspase 9 and procaspase 3. Asthmatic serum induced Akt and ERK phosphorylation, and ERK activation was suppressed by AKTi. Taken together, asthmatic serum inhibited normal eosinophil apoptosis via PI3K/Akt/ERK cascade. The novel approach taken in this study provided better insight into HDM-associated anti-apoptotic mechanism of eosinophils in patients with asthma.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2011-0013716).

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Authors and Affiliations

  1. Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Taejon, 301-746, Republic of Korea

    In Sik Kim, Mi Jin Kim & Do Hyung Kim

  2. Department of Clinical Laboratory Science, Wonkwang Health Science University, 501, Iksandaero, Iksan, 570-750, Republic of Korea

    Mi Jin Kim & Ji-Sook Lee

  3. Department of Respiratory Internal Medicine, College of Medicine, Konyang University, Taejon, 302-718, Republic of Korea

    Eugene Choi

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  1. In Sik Kim
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  2. Mi Jin Kim
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Correspondence to Ji-Sook Lee.

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Kim, I.S., Kim, M.J., Kim, D.H. et al. Different anti-apoptotic effects of normal and asthmatic serum on normal eosinophil apoptosis depending on house dust mite-specific IgE. Mol Biol Rep 40, 5875–5881 (2013). https://doi.org/10.1007/s11033-013-2695-z

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  • DOI: https://doi.org/10.1007/s11033-013-2695-z

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