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In vivo study on the effects of curcumin on the expression profiles of anti-tumour genes (VEGF, CyclinD1 and CDK4) in liver of rats injected with DEN

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Abstract

In this study we investigated the effects of curcumin, derived from plant Curcuma longa, on oxidative toxicity, and the possible molecular mechanism of antitumour of curcumin in liver cancer rats. Results showed that blood levels of Gamma-glutamyltransferase, aspartate aminotransferase, alanine aminotransferase, glutathione S-transferase, and liver level of MD were significantly decreased after curcumin feeding. Levels of the liver malondialdehyde MDA, nitric oxide and antioxidant enzymes were significantly increased. Moreover, RT-PCR and Western blot analysis results showed that curcumin treatment significantly decreased liver vascular endothelial growth factor (VEGF), CyclinD1 and CDK4 mRNA expression levels and CyclinD1 and CDK4 proteins levels in liver cancer rats. These findings were confirmed by histopathology. It is concluded that curcumin can protect the liver from the damage caused by N-nitrosodiethylamine. Moreover, curcumin has the potential to be used in a therapy for liver cancer. The present data provide evidence to support the presence of free radicals and VEGF, CyclinD1 and CDK4 mRNA in rat tumour cells. Studies are in progress in order to further characterize the role of VEGF, CyclinD1 and CDK4 mRNA in liver cancer cells and in hepatic therapeutics.

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Authors and Affiliations

  1. Department of Pharmacy, The First Affiliated Hospital of Shantou University Medical College, Shantou, People’s Republic of China

    Chu Zhu Huang & Dan Ling Tang

  2. Department of Cardiac Pulmonary Surgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, People’s Republic of China

    Wei Zhe Huang

  3. Departments of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, People’s Republic of China

    Ge Zhang

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  1. Chu Zhu Huang
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  2. Wei Zhe Huang
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  4. Dan Ling Tang
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Correspondence to Dan Ling Tang.

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Huang, C.Z., Huang, W.Z., Zhang, G. et al. In vivo study on the effects of curcumin on the expression profiles of anti-tumour genes (VEGF, CyclinD1 and CDK4) in liver of rats injected with DEN. Mol Biol Rep 40, 5825–5831 (2013). https://doi.org/10.1007/s11033-013-2688-y

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  • DOI: https://doi.org/10.1007/s11033-013-2688-y

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