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High yield of cells committed to the photoreceptor-like cells from conjunctiva mesenchymal stem cells on nanofibrous scaffolds

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An Erratum to this article was published on 04 June 2013

Abstract

Transplantation of stem cells using biodegradable and biocompatible nanofibrous scaffolds is a promising therapeutic approach for treating inherited retinal degenerative diseases such as retinitis pigmentosa and age-related macular degeneration. In this study, conjunctiva mesenchymal stem cells (CJMSCs) were seeded onto poly-l-lactic acid (PLLA) nanofibrous scaffolds and were induced to differentiate toward photoreceptor cell lineages. Furthermore, the effects of orientation of scaffold on photoreceptor differentiation were examined. Scanning electron microscopy (SEM) imaging, quantitative real time RT-PCR (qPCR) and immunocytochemistry were used to analyze differentiated cells and their expression of photoreceptor-specific genes. Our observations demonstrated the differentiation of CJMSCs to photoreceptor cells on nanofibrous scaffolds and suggested their potential application in retinal regeneration. SEM imaging showed that CJMSCs were spindle shaped and well oriented on the aligned nanofiber scaffolds. The expression of rod photoreceptor-specific genes was significantly higher in CJMSCs differentiated on randomly-oriented nanofibers compared to those on aligned nanofibers. According to our results we may conclude that the nanofibrous PLLA scaffold reported herein could be used as a potential cell carrier for retinal tissue engineering and a combination of electrospun nanofiber scaffolds and MSC-derived conjunctiva stromal cells may have potential application in retinal regenerative therapy.

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Acknowledgments

We are very grateful to manager of Stem Cell Technology Research Center. This article is withdrawn from a Ph.D course.

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Authors and Affiliations

  1. Medical Physics and Biomedical Engineering Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Samad Nadri

  2. Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Samad Nadri

  3. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran

    Samad Nadri

  4. Biotechnology Department, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Bahram Kazemi

  5. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Bahram Kazemi

  6. Department of Stem Cell and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

    Mohamadreza Baghaban Eeslaminejad

  7. Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Shahin Yazdani

  8. Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University, Jallal Ale Ahmad Avenue, P.O. Box 14115-111, Tehran, Iran

    Masoud Soleimani

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  1. Samad Nadri
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Corresponding authors

Correspondence to Bahram Kazemi or Masoud Soleimani.

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11033_2012_2360_MOESM1_ESM.jpg

Supplementary Fig. 1. Morphological characteristics and in vitro differentiation of conjunctiva stromal fibroblast-like cells into mesenchymal lineages: (A) conjunctiva stromal cells (passage 5) have differentiated into (B) mineralizing cells stained with alizarin red (C) Adipocytes stained with Oil red O, chondrocytic lineage stained with Alcian blue (D). (Magnification = ×20). (JPEG 161 kb)

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Nadri, S., Kazemi, B., Eeslaminejad, M.B. et al. High yield of cells committed to the photoreceptor-like cells from conjunctiva mesenchymal stem cells on nanofibrous scaffolds. Mol Biol Rep 40, 3883–3890 (2013). https://doi.org/10.1007/s11033-012-2360-y

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  • DOI: https://doi.org/10.1007/s11033-012-2360-y

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