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NF-κB controls Il2 and Csf2 expression during T cell development and activation process

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Abstract

Aging and dysregulation of immune responds are closely associated through a complicated but unclear mechanism. Although many theories have been proposed as overall dysregulation involved in aging, mechanisms such as efficiency of DNA repairing, over-expression of transcription factors (such as NF-κB family), and shift of cell types, are among many factors that contribute to and affect aging process. It is of great interests to understand the possible mechanism that is involved in aging immune system. Here, we report that the inducible genes Il2 and Csf2 are increased as T cells undergo activation and aging. Of particular note were the findings that the relative composition of the circulating CD4+ T cell population changes as animals mature with an increased percentage of the population being memory/effector type cells. In addition, mRNA levels of NF-κB family genes that are essential elements for cytokine activation in adult mice and activated T cells are significantly increased. We have demonstrated that the expression of inducible genes is accompanied by increased memory/effector type cells and by increased expression level of NF-κB family genes during cell activation and development.

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Abbreviations

PMA/I:

Phorbol 12-myristate 13-acetate and calcium ionophore

FACS:

Fluorescent-activated cell sorting

Il2 :

Interleukin-2

Csf2 :

Granulocyte-macrophage colony-stimulating factor

NF-κB:

Nuclear factor kappa-light-chain-enhancer of activated B cells

Sirt:

Sirtuin

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Acknowledgments

This work was funded by an Epigenomics Capacity Development Grant from Bioplatforms Australia (http://www.bioplatforms.com.au/). Project supported by the National Science Foundation of China (Grant No. 31172185) for Chao Sun. A scholarship from the Chinese Scholarship Council was awarded to YL. The authors declare that they have no competing financial interests. This funding does not alter our adherence to all the MBR policies on sharing data and materials.

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Authors and Affiliations

  1. College of Animal Science & Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Yan Li & Chao Sun

  2. Laboratory of Immunology and Inflammation, Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, People’s Republic of China

    Yan Li

  3. Department of Genome Biology, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, 2601, Australia

    Yan Li & Junyao Fan

  4. ACRF Biomolecular Resource Facility, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, 2601, Australia

    Stephen J. Ohms

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  1. Yan Li
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Correspondence to Chao Sun or Junyao Fan.

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Li, Y., Ohms, S.J., Sun, C. et al. NF-κB controls Il2 and Csf2 expression during T cell development and activation process. Mol Biol Rep 40, 1685–1692 (2013). https://doi.org/10.1007/s11033-012-2219-2

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  • DOI: https://doi.org/10.1007/s11033-012-2219-2

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