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Cloning and evolutionary analysis of tobacco MAPK gene family

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Abstract

The mitogen-activated protein (MAP) kinase cascade is an important signaling module which is involved in biotic and abiotic stress responses as well as plant growth and development. In this study, we identified 17 tobacco MAPKs including 11 novel tobacco MAPK genes that have not been identified before. Comparative analysis with MAPK gene families from other plants, such as Athaliana thaliana, rice and poplar, suggested that tobacco MAPKs (such as NtMPK1, NtMPK3 and NtMPK8) might play similar functions in response to abiotic and biotic stresses. QRT-PCR analysis revealed that a total of 14 NtMPKs were regulated by SA and/or MeJA, suggesting their potential roles involved in plant defense response. In addition, 6 NtMPKs were induced by drought treatment, implying their roles in response to drought stress. Our results indicated that most of tobacco MAPK might be involved in plant defense response, which provides the basis for further analysis on physiological functions of tobacco MAPKs.

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Acknowledgments

This work was supported by grants from the National Basic Research Program of China (No. 2012CB114600), Fundamental Research Funds for the Central Universities (No. CDJZR10290003), the National Natural Science Foundation (No. 30901054, 31001034 and 31000563) and Key Project for National Tobacco Government (No. 110200902037).

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Authors and Affiliations

  1. The Institute of Agricultural and Life Science, Chongqing University, Chongqing, 400030, China

    Xingtan Zhang & Yafei Yan

  2. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China

    Tingcai Cheng, Genhong Wang & Qingyou Xia

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  1. Xingtan Zhang
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  2. Tingcai Cheng
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  3. Genhong Wang
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  5. Qingyou Xia
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Correspondence to Qingyou Xia.

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Supplementary Fig. 1

Full-length protein sequence alignment of tobacco MAPKs. The CDdomains were marked by a red box (GIF 97 kb)

Supplementary Fig. 2

Expression of PR1a and ODC under treatments of SA and MeJA (JPG 31 kb)

Supplementary Table 1

The primer sequences used in RT-PCR for cloning of 11 novol NtMPKs. (DOC 24 kb)

Supplementary Table 2

The primer sequences used in qRT-PCR for detection of NtMPK expression. (DOC 37 kb)

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Zhang, X., Cheng, T., Wang, G. et al. Cloning and evolutionary analysis of tobacco MAPK gene family. Mol Biol Rep 40, 1407–1415 (2013). https://doi.org/10.1007/s11033-012-2184-9

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  • DOI: https://doi.org/10.1007/s11033-012-2184-9

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