Abstract
Our study used MHC class IIb gene exon3 complete sequence as markers to investigate genetic variability, selection and population differentiation in Chinese alligator. In this study, 282 bp MHC IIb exon3 complete sequence was got, none of the sequences contained insertions/deletions or stop codons, suggesting that all sequences might come from functional molecules in the genome. The neighbor-joining (NJ) tree revealed that Xuangzhou and Changxing populations were genetically close related, while Wild population showed the most diverse from the other. Gene flow (Nm) was very higher than one, suggesting that inter-group gene flow may have occurred. Furthermore, the results showed that MHC IIb gene might be a good molecular marker, we think that this technology could be used for Chinese alligator breeding and releasing in future.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (NSFC, No. 30770312), the Excellent Creative Research Team of Animal Biology in Anhui Normal University, the Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province and Zoology Practical Skills Training Center in Fuyang Teachers College. We are grateful to the assistance of Anhui Research Center for Chinese Alligator Reproduction in this study.
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Nie, C., Zhao, J., Li, Y. et al. Diversity and selection of MHC class IIb gene exon3 in Chinese alligator. Mol Biol Rep 40, 295–301 (2013). https://doi.org/10.1007/s11033-012-2061-6
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DOI: https://doi.org/10.1007/s11033-012-2061-6