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Evaluation of synthetic hexaploid wheats (derivative of durum wheats and Aegilops tauschii accessions) for studying genetic diversity using randomly amplified polymorphic DNA (RAPD) markers

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Abstract

Synthetic hexaploid (SH) wheat derived from crossing tetraploid durum wheat and diploid Aegilops tauschii provide germplasm for wheat improvement as the conventional wheat varieties possess very low genetic diversity. This study aims to identify diverse SH lines which can be used in breeding programs for transferring the desired traits into bread wheat. The study was conducted on 24 SH lines using 10 pairs of randomly amplified polymorphic DNA (RAPD) markers. Application of RAPDs showed the presence of some diagnostic bands in SH wheats that were absent in durum parents suggest that these bands are donated by D genome of the wild relative Ae. tauschii.

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Authors and Affiliations

  1. Department of Biotechnology, Abdul Wali Khan University Palosa Campus, Charsadda, Pakistan

    Muhammad Shakeel & Muhammad Ilyas

  2. National Agriculture Research Centre (NARC), Islamabad, Pakistan

    Mujeeb Kazi

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  1. Muhammad Shakeel
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  2. Muhammad Ilyas
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  3. Mujeeb Kazi
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Correspondence to Muhammad Ilyas.

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Shakeel, M., Ilyas, M. & Kazi, M. Evaluation of synthetic hexaploid wheats (derivative of durum wheats and Aegilops tauschii accessions) for studying genetic diversity using randomly amplified polymorphic DNA (RAPD) markers. Mol Biol Rep 40, 21–26 (2013). https://doi.org/10.1007/s11033-012-1943-y

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  • DOI: https://doi.org/10.1007/s11033-012-1943-y

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