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Comparative analysis of human reproductive proteomes identifies candidate proteins of sperm maturation

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Abstract

Male reproductive proteomes provide basis for studying gene products and its involvement or regulation in sperm physiology. Here, a comparative study between these proteomes was performed to find potential proteins and functions associated with human sperm maturation. Seven reproductive proteomes associated with human sperm physiology were integrated. Gene ontology analysis were performed using DAVID and Panther tools to determine enriched functions. Total of 270 proteins overlapped between epididymal, prostatic milieu and sperm proteome were thought to be candidate proteins involved in sperm maturation, and they showed enriched functions of proteasomal protein catabolic process and protein folding. 34 epididymal milieu proteins and 274 prostatic milieu proteins were contributed to the composition of seminal fluids proteome. Literatures have confirmed the involvements in sperm maturation of many of these proteins The spatial expressions of 24 epididymal milieu proteins involved in chaperone and antioxidant activity were authenticated by real-time RT-PCR. These proteins may serve as candidate molecules for future studies of sperm maturation and male infertility.

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References

  1. Gatti JL, Castella S, Dacheux F et al (2004) Post-testicular sperm environment and fertility. Anim Reprod Sci 82–83:321–339

    Article  PubMed  Google Scholar 

  2. Cooper TG (2007) Sperm maturation in the epididymis: a new look at an old problem. Asian J Androl 9:533–539

    Article  PubMed  CAS  Google Scholar 

  3. Marengo SR (2008) Maturing the sperm: unique mechanisms for modifying integral proteins in the sperm plasma membrane. Anim Reprod Sci 105:52–63

    Article  PubMed  CAS  Google Scholar 

  4. Cornwall GA (2009) New insights into epididymal biology and function. Hum Reprod Update 15:213–227

    Article  PubMed  CAS  Google Scholar 

  5. Turner TT (2008) De Graaf’s thread: the human epididymis. J Androl 29:237–250

    Article  PubMed  Google Scholar 

  6. Li J, Liu F, Liu X, et al. (2011) Mapping of the human testicular proteome and its relationship with that of the epididymis and spermatozoa. Mol Cell Proteomics 10: M110.004630

  7. Li J, Liu F, Wang H et al (2010) Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins. Mol Cell Proteomics 9:2517–2528

    Article  PubMed  CAS  Google Scholar 

  8. Duncan MW, Thompson HS (2007) Proteomics of semen and its constituents. Proteomics Clin Appl 1:861–875

    Article  PubMed  CAS  Google Scholar 

  9. Gonzales GF (2001) Function of seminal vesicles and their role on male fertility. Asian J Androl 3:251–258

    PubMed  CAS  Google Scholar 

  10. Drake RR, Elschenbroich S, Lopez-Perez O et al (2010) In-depth proteomic analyses of direct expressed prostatic secretions. J Proteome Res 9:2109–2116

    Article  PubMed  CAS  Google Scholar 

  11. Thimon V, Frenette G, Saez F et al (2008) Protein composition of human epididymosomes collected during surgical vasectomy reversal: a proteomic and genomic approach. Hum Reprod 23:1698–1707

    Article  PubMed  CAS  Google Scholar 

  12. Utleg AG, Yi EC, Xie T et al (2003) Proteomic analysis of human prostasomes. Prostate 56:150–161

    Article  PubMed  CAS  Google Scholar 

  13. Pilch B, Mann M (2006) Large-scale and high-confidence proteomic analysis of human seminal plasma. Genome Biol 7:R4

    Article  Google Scholar 

  14. Saez F, Frenette G, Sullivan R (2003) Epididymosomes and prostasomes: their roles in posttesticular maturation of the sperm cells. J Androl 24:149–154

    PubMed  Google Scholar 

  15. Sullivan R, Frenette G, Girouard J (2007) Epididymosomes are involved in the acquisition of new sperm proteins during epididymal transit. Asian J Androl 9:483–491

    Article  PubMed  CAS  Google Scholar 

  16. Dacheux JL, Castella S, Gatti JL et al (2005) Epididymal cell secretory activities and the role of proteins in boar sperm maturation. Theriogenology 63:319–341

    Article  PubMed  CAS  Google Scholar 

  17. Baker MA (2011) The ‘omics revolution and our understanding of sperm cell biology. Asian J Androl 13:6–10

    Article  PubMed  CAS  Google Scholar 

  18. Baker MA, Reeves G, Hetherington L et al (2007) Identification of gene products present in Triton X-100 soluble and insoluble fractions of human spermatozoa lysates using LC–MS/MS analysis. Proteomics Clin Appl 1:524–532

    Article  PubMed  CAS  Google Scholar 

  19. Brewis IA, Gadella BM (2010) Sperm surface proteomics: from protein lists to biological function. Mol Hum Reprod 16:68–79

    Article  PubMed  CAS  Google Scholar 

  20. Aitken RJ, Baker MA (2008) The role of proteomics in understanding sperm cell biology. Int J Androl 31:295–302

    Article  PubMed  CAS  Google Scholar 

  21. Aitken RJ, Nixon B, Lin M et al (2007) Proteomic changes in mammalian spermatozoa during epididymal maturation. Aian J Androl 9:554–556

    Article  CAS  Google Scholar 

  22. Ren D (2011) Calcium signaling in sperm: help from prostasomes. Sci Signal 4: p e27

  23. Nöthling JO, Gerber D, Colenbrander B et al (2007) The effect of homologous prostatic fluid on motility and morphology of dog epididymal spermatozoa extended and frozen in Biladyl with Equex STM paste or Andromed. Theriogenology 67:264–275

    Article  PubMed  Google Scholar 

  24. Aumüller G, Riva A (1992) Morphology and functions of the human seminal vesicle. Andrologia 24:183–196

    Article  PubMed  Google Scholar 

  25. Edwards DR, Handsley MM, Pennington CJ (2008) The ADAM metalloproteinases. Mol Aspects Med 29:258–289

    Article  PubMed  CAS  Google Scholar 

  26. Mandal A, Bhattacharyya AK (1990) Biochemical composition of washed human seminal coagulum in comparison to sperm-free semen from the same donors. J Reprod Fertil 88:113–118

    Article  PubMed  CAS  Google Scholar 

  27. Baker MA, Aitken RJ (2009) Proteomic insights into spermatozoa: critiques, comments and concerns. Expert Rev Proteomics 6:691–705

    Article  PubMed  CAS  Google Scholar 

  28. Dun MD, Aitken RJ, Nixon B (2012) The role of molecular chaperones in spermatogenesis and the post-testicular maturation of mammalian spermatozoa. Hum Reprod Update 18:420–435

    Article  PubMed  Google Scholar 

  29. Drevet JR (2006) The antioxidant glutathione peroxidase family and spermatozoa: a complex story. Mol Cell Endocrinol 250:70–79

    Article  PubMed  CAS  Google Scholar 

  30. Vernet P, Aitken RJ, Drevet JR (2004) Antioxidant strategies in the epididymis. Mol Cell Endocrinol 216:31–39

    Article  PubMed  CAS  Google Scholar 

  31. Sutovsky P (2011) Sperm proteasome and fertilization. Reproduction 142:1–14

    Article  PubMed  CAS  Google Scholar 

  32. Baska KM, Manandhar G, Feng D et al (2008) Mechanism of extracellular ubiquitination in the mammalian epididymis. J Cell Physiol 215:684–696

    Article  PubMed  CAS  Google Scholar 

  33. Cornwall GA, von Horsten HH, Swartz D et al (2007) Extracellular quality control in the epididymis. Asian J Androl 9:500–507

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Central Laboratory, Yu-Huang-Ding Hospital/Qingdao University, Yantai, 264000, Shandong, People’s Republic of China

    Liu Fu-Jun & Shen Xiao-Fang

  2. Reproduction Medical Center, Yu-Huang-Ding Hospital/Qingdao University, Yantai, 264000, Shandong, People’s Republic of China

    Shen Xiao-Fang

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  1. Liu Fu-Jun
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  2. Shen Xiao-Fang
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Correspondence to Shen Xiao-Fang.

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Fu-Jun, L., Xiao-Fang, S. Comparative analysis of human reproductive proteomes identifies candidate proteins of sperm maturation. Mol Biol Rep 39, 10257–10263 (2012). https://doi.org/10.1007/s11033-012-1902-7

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  • DOI: https://doi.org/10.1007/s11033-012-1902-7

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