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−1131T>C and SW19 polymorphisms in APOA5 gene and lipid levels in type 2 diabetic patients

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Abstract

Type 2 diabetes mellitus is a metabolic, vascular, and neuropathic disease with a high risk of atherosclerotic events due to dyslipidemic states. Polymorphisms in Apolipoprotein A5 gene (APOA5) have been associated with increased triglyceride levels in many different populations. This study aimed to identify the frequencies of the APOA5 −1131T>C and SW19 polymorphisms and evaluate their effects on lipid levels in patients with type 2 diabetes. Genotyping of APOA5 −1131T>C and SW19 polymorphisms was performed by PCR–RFLP in 146 diabetic patients and in controls (n = 173), from 30 to 80 years of age. Diabetic patients were divided into two groups: patients not treated with lipid lowering drugs (group G1; n = 62) and those treated with lipid lowering drugs (group G2, n = 84). Lipids and lipoproteins were determined enzymatically. Among participants not treated with lipid-lowering drugs (diabetics G1 and controls; n = 235), the −1131C was associated with lower LDLc levels (p = 0.015). In the diabetic patients, the 19W allele was associated with higher triglyceride levels (p = 0.004). In G1 diabetic patients, the combined analysis of APOA5 −1131T>C and SW19 polymorphisms showed that [TC or CC] + SS carriers presented lower total cholesterol levels than did other genotype combinations (p = 0.049). It could therefore be concluded that APOA5 −1131T>C and SW19 polymorphisms influence lipid levels in type 2 diabetic patients.

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Acknowledgments

We acknowledge the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado Minas Gerais (FAPEMIG) and Pró-Reitoria de Pesquisa da Universidade Federal de Minas Gerais (UFMG) for financial support. We thank Fátima, Márcio and Eunice (Department of Clinical and Toxicological Analysis of the Faculty of Pharmacy of UFMG). Most of all, thanks to all patients and healthy volunteers for their selfless donation of samples used in this study.

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Authors and Affiliations

  1. Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Antônio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais, 31270-901, Brazil

    Mirelle O. Sóter, Karina B. Gomes, Ana P. Fernandes, Maria das G. Carvalho, Poliana S. Pinheiro & Marinez O. Sousa

  2. Post-Graduation and Research Unit, Santa Casa Hospital, Belo Horizonte, Brazil

    Adriana A. Bosco & Daniel D. R. Silva

Authors
  1. Mirelle O. Sóter
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  2. Karina B. Gomes
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  3. Ana P. Fernandes
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  4. Maria das G. Carvalho
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  5. Poliana S. Pinheiro
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  6. Adriana A. Bosco
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  7. Daniel D. R. Silva
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  8. Marinez O. Sousa
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Correspondence to Karina B. Gomes.

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Sóter, M.O., Gomes, K.B., Fernandes, A.P. et al. −1131T>C and SW19 polymorphisms in APOA5 gene and lipid levels in type 2 diabetic patients. Mol Biol Rep 39, 7541–7548 (2012). https://doi.org/10.1007/s11033-012-1588-x

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  • DOI: https://doi.org/10.1007/s11033-012-1588-x

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