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Co-expression of Oct-4 and Nestin in human breast cancers

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Abstract

The aim is to investigate the clinical implications of the Oct-4 and Nestin protein in human breast cancers. A total of 346 cases including 26 fresh and 320 paraffin-embedded tumor tissues were selected for characterizing the frequency of CD44+CD24− tumor cells by flow cytometry and the differential expression of the stem cell-related genes between CD44+CD24− and non-CD44+CD24− tumor cells was analyzed by PCR Array and immunofluorescence. In comparison with the non-CD44+CD24− tumor cells, the CD44+CD24−, particularly for those with high percentage of Oct-4+ and Nestin+, tumor cells had higher tumorigenicity by forming mammospheres in vitro. More importantly, 42 (13.125%) out of 320 tumor tissues were positive for Oct-4 and Nestin staining. Universal analysis and multivariate analysis revealed that the expression of Oct-4 and Nestin was associated significantly with younger age, pathogenic degrees, lymph node metastasis and triple-negative breast cancer independently (P < 0.05) as well as shorter survival (P = 0.001). Oct-4 and Nestin were important regulators of the development of breast cancer, and Oct-4 and Nestin may be used as predictors for the prognosis of breast cancers.

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Acknowledgment

This study was supported by the grant from the China National Natural Science Foundation (No. 81102029 and 81172047).

Conflict of interests

The authors stated no conflict of interest.

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Authors and Affiliations

  1. Department of Breast Surgery, General Surgery, The First Hospital of China Medical University, Liaoning Province, Shenyang, 110001, China

    Caigang Liu, Yanjun Zhang, Yunfei Wu, Ping Lu & Feng Jin

  2. Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, 110001, China

    Xuezhao Cao

  3. Department of Breast Surgery, Tumor Hospital of Liaoning Province, Shenyang, 110000, China

    Hong Xu & Ruishan Zhang

Authors
  1. Caigang Liu
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  2. Xuezhao Cao
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  3. Yanjun Zhang
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  4. Hong Xu
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  5. Ruishan Zhang
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  6. Yunfei Wu
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  7. Ping Lu
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  8. Feng Jin
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Corresponding author

Correspondence to Feng Jin.

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Caigang Liu and Xuezhao Cao were co-first authors.

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Liu, C., Cao, X., Zhang, Y. et al. Co-expression of Oct-4 and Nestin in human breast cancers. Mol Biol Rep 39, 5875–5881 (2012). https://doi.org/10.1007/s11033-011-1398-6

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  • DOI: https://doi.org/10.1007/s11033-011-1398-6

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