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Gene expression profiling of Sinapis alba leaves under drought stress and rewatering growth conditions with Illumina deep sequencing

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Abstract

Sinapis alba has many desirable agronomic traits including tolerance to drought. In this investigation, we performed the genome-wide transcriptional profiling of S. alba leaves under drought stress and rewatering growth conditions in an attempt to identify candidate genes involved in drought tolerance, using the Illumina deep sequencing technology. The comparative analysis revealed numerous changes in gene expression level attributable to the drought stress, which resulted in the down-regulation of 309 genes and the up-regulation of 248 genes. Gene ontology analysis revealed that the differentially expressed genes were mainly involved in cell division and catalytic and metabolic processes. Our results provide useful information for further analyses of the drought stress tolerance in Sinapis, and will facilitate molecular breeding for Brassica crop plants.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (30671312), the Natural Science Foundation of Hubei Province (2008CDA083 and 2009CDB191), the Natural Science Foundation of Henan Province (114100510013), the Chenguang Program of Wuhan City (201050231022), the International Science and Technology Cooperation Item (S2012GR0080), and the Science and Technical Innovation Project of Hubei Province.

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Authors and Affiliations

  1. Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Oil Crop Biology and Genetic Breeding of the Ministry of Agriculture, Wuhan, 430062, China

    Cai-Hua Dong, Xiao-Hong Yan, Shun-Mou Huang, Li-Jun Wang, Rui-Xing Guo, Guang-Yuan Lu, Xue-Kun Zhang, Xiao-Ping Fang & Wen-Hui Wei

  2. College of Food Science and Technology, Agricultural University of Hebei, Baoding, 071001, China

    Chen Li

  3. Department of Bioengineering, Zhengzhou University, Zhengzhou, 450001, China

    Jin-Yong Huang

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  1. Cai-Hua Dong
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Correspondence to Xue-Kun Zhang, Xiao-Ping Fang or Wen-Hui Wei.

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Cai-Hua Dong, Chen Li and Xiao-Hong Yan contributed equally to this work.

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Dong, CH., Li, C., Yan, XH. et al. Gene expression profiling of Sinapis alba leaves under drought stress and rewatering growth conditions with Illumina deep sequencing. Mol Biol Rep 39, 5851–5857 (2012). https://doi.org/10.1007/s11033-011-1395-9

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  • DOI: https://doi.org/10.1007/s11033-011-1395-9

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