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Polymorphism in the exon 4 of β-lactoglobulin variant B precursor gene and its association with milk traits and protein structure in Chinese Holstein

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Abstract

β-lactoglobulin (β-LG) is the major whey protein in the milk. In order to investigate the polymorphism of β-LG variant B precursor (β-LG B*: GenBank accession no. DQ489319) gene and its effects on the milk traits, the single-strand conformation polymorphism method (PCR-SSCP) were adopted to analyze polymorphism between 5229th and 5476th bp in the β-LG B* gene in Chinese Holstein. Four genotypes were found (AA, AB, AC and ABC) and 3 single nucleotide polymorphisms (SNPs) were detected (g.5239C>A, g.5240A>C, g.5305C>T and mix type g.5305C/T) in the exon 4 of β-LG B* gene. It was also found that the protein contents of AB, AC and ABC dairy cows were higher than AA (P < 0.05), and AC cows were the highest among them. Three SNPs (g.5239C>A, g.5240A>C and g.5305C>T) might affect the milk trait and all of them were high polymorphism (0.5 < PIC < 1.0). In further researches, the three SNPs also caused amino acid change (Asp>Glu, Thr>Pro and Ala>Val) respectively, and the spatial secondary and tertiary structure forecasting result also showed that single amino acid change influence protein spatial structure change in Chinese Holstein. Taken together, it is suggested that these SNPs change β-LG B* gene structure and expression. The polymorphism possibly holds the secret of milk protein and fat contents in the milk of Chinese Holstein.

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References

  1. Ganai NA, Bovenhuis H, van Arendonk JA, Visker MH (2009) Novel polymorphisms in the bovine beta-lactoglobulin gene and their effects on beta-lactoglobulin protein concentration in milk. Anim Genet 40:127–133

    Article  PubMed  CAS  Google Scholar 

  2. Rusu D, Drouin R, Pouliot Y, Gauthier S, Poubelle PE (2009) A bovine whey protein extract can enhance innate immunity by priming normal human blood neutrophils. J Nutr 139(2):386–393

    PubMed  CAS  Google Scholar 

  3. Braunschweig MH (2007) Short communication: duplication in the 5′-flanking region of the [beta]-lactoglobulin gene is linked to the BLG A allele. J Dairy Sci 90(12):5780–5783

    Article  PubMed  CAS  Google Scholar 

  4. Paterson GR, Otter DE, Hill JP (1995) Application of capillary electrophoresis in the identification of phenotypes containing the [beta]-lactoglobulin C variant. J Dairy Sci 78:2637–2644

    Article  PubMed  CAS  Google Scholar 

  5. Tsiaras AM, Bargouli GG, Banos G, Boscos CM (2005) Effect of Kappa-casein and Beta-lactoglobulin loci on milk production traits and reproductive performance of Holstein cows. J Dairy Sci 88:327–334

    Article  PubMed  CAS  Google Scholar 

  6. Ramos AM, Matos CAP, Russo-Almeida PA, Bettencourt CMV, Matos J et al (2009) Candidate genes for milk production traits in Portuguese dairy sheep. Small Rumin Res 82:117–121

    Article  Google Scholar 

  7. Auldist MJ, Thomson NA, Mackle TR, Hill JP, Prosser CG (2000) Effects of pasture allowance on the yield and composition of milk from cows of different [beta]-lactoglobulin phenotypes. J Dairy Sci 83:2069–2074

    Article  PubMed  CAS  Google Scholar 

  8. Ron M, Yoffe O, Ezra E, Medrano JF, Weller JI (1994) Determination of effects of milk protein genotype on production traits of Israeli Holsteins. J Dairy Sci 77(4):1106–1113

    Article  PubMed  CAS  Google Scholar 

  9. Kumar A, Rout PK, Roy R (2006) Polymorphism of beta-lactoglobulin gene in Indian goats and its effect on milk yield. J Appl Genet 47:49–53

    Article  PubMed  Google Scholar 

  10. Ng-Kwai-Hang KF, Hayes JF, Moxley JE, Monardes HG (1987) Variation in milk protein concentrations associated with genetic polymorphism and environmental factors. J Dairy Sci 70:563–570

    Article  PubMed  CAS  Google Scholar 

  11. Rachagani S, Gupta ID, Gupta N, Gupta SC (2006) Genotyping of beta-lactoglobulin gene by PCR-RFLP in Sahiwal and Tharparkar cattle breeds. BMC Genet 7:31

    Article  PubMed  Google Scholar 

  12. Braunschweig MH, Leeb T (2006) Aberrant low expression level of bovine [beta]-lactoglobulin is associated with a c to a transversion in the blg promoter region. J Dairy Sci 89:4414–4419

    Article  PubMed  CAS  Google Scholar 

  13. Kelley LA, Sternberg MJE (2009) Protein structure prediction on the Web: a case study using the Phyre server. Nat Protoc 4:363–371

    Article  PubMed  CAS  Google Scholar 

  14. Arnold K, Bordoli L, Kopp J, Schwede T (2006) The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22:195–201

    Article  PubMed  CAS  Google Scholar 

  15. Benkert P, Schwede T, Tosatto SC (2009) QMEANclust: estimation of protein model quality by # combining a composite scoring function with structural density information. BMC Struct Biol 20:9–35

    Google Scholar 

  16. Melo F, Feytmans E (1998) Assessing protein structures with a non-local atomic interaction energy. J Mol Biol 277:1141–1152

    Article  PubMed  CAS  Google Scholar 

  17. Benkert P, Silvio CE, Tosatto, Schomburg D (2008) QMEAN: a comprehensive scoring function for model quality assessment. Proteins Struct Funct Bioinform 71:261–277

    Article  CAS  Google Scholar 

  18. Hooft RWW, Vriend G, Sander C, Abola EE (1996) Errors in protein structures. Nature 381:272–273

    Article  PubMed  CAS  Google Scholar 

  19. Hutchinson EG, Thornton JM (1996) PROMOTIF—a program to identify structural motifs in proteins. Protein Sci 5:212–220

    Article  PubMed  CAS  Google Scholar 

  20. Laskowski RA, MacArthur MW, Moss D, Thornton JM (1993) PROCHECK: a program to check the stereochemical quality of protein structures. J Appl Cryst 26:283–291

    Article  CAS  Google Scholar 

  21. O’Halloran F, Berry DP, Bahar B, Howard DJ, Sweeney T et al (2010) Polymorphisms in the bovine lactoferrin promoter are associated with reproductive performance and somatic cell count. J Dairy Sci 93:1253–1259

    Article  PubMed  Google Scholar 

  22. Huang J, Wang H, Wang C, Li J, Li Q et al (2010) Single nucleotide polymorphisms, haplotypes and combined genotypes of lactoferrin gene and their associations with mastitis in Chinese Holstein cattle. Mol Biol Rep 37:477–483

    Article  PubMed  CAS  Google Scholar 

  23. Corral JM, Padilla JA, Izquierdo M (2010) Associations between milk protein genetic polymorphisms and milk production traits in Merino sheep breed. Livest Sci 129:73–79

    Article  Google Scholar 

  24. Arora R, Bhatia S, Mishra BP, Sharma R, Pandey AK et al (2010) Genetic polymorphism of the beta-lactoglobulin gene in native sheep from India. Biochem Genet 48:304–311

    Article  PubMed  CAS  Google Scholar 

  25. Wang HM, Kong ZX, Wang CF, Huang JM, Li QL et al (2009) Genetic polymorphism in 5′-flanking region of the lactoferrin gene and its associations with mastitis in Chinese Holstein cows. Yi Chuan 31(4):393–399

    Article  PubMed  CAS  Google Scholar 

  26. Cho Y, Batt CA, Sawyed L (1994) Probing the retinol-binding sitoef bovine β-lactoglobuln. J Biol Chem 269(15):11102–11107

    PubMed  CAS  Google Scholar 

  27. Li L, Xu W, Li N (2009) DNA methylation changes in cell line from beta-lactoglobulin gene targeted fetus. Anim Reprod Sci 112:402–408

    Article  PubMed  CAS  Google Scholar 

  28. Dario C, Carnicella D, Dario M, Bufano G (2008) Genetic polymorphism of β-lactoglobulin gene and effect on milk composition in Leccese sheep. Small Rumin Res 74:270–273

    Article  Google Scholar 

  29. Celik S (2003) [beta]-Lactoglobulin genetic variants in brown Swiss breed and its association with compositional properties and rennet clotting time of milk. Int Dairy J 13:727–731

    Article  CAS  Google Scholar 

  30. Van Eenennaam A, Medrano JF (1991) Milk protein polymorphisms in California dairy cattle. J Dairy Sci 74:1730–1742

    Article  Google Scholar 

  31. Ng-Kwai-Hang KF, Monardes HG, Hayes JF (1990) Association between genetic polymorphism of milk proteins and production traits during three lactations. J Dairy Sci 73:3414–3420

    Article  CAS  Google Scholar 

  32. Zhang F, Khajavi M, Connolly AM, Towne CF, Batish SD et al (2009) The DNA replication FoSTeS/MMBIR mechanism can generate genomic, genic and exonic complex rearrangements in humans. Nat Genet 41:849–853

    Article  PubMed  CAS  Google Scholar 

  33. Durbin RM (2010) A map of human genome variation from population-scale sequencing. Nature 467:1061–1073

    Article  CAS  Google Scholar 

  34. Papiz MZ, Sawyer L, Eliopoulos EE, North ACT, Findlay JBC et al (1986) The structure of beta-lactoglobulin and its similarity to plasma retinol-binding protein. Nature 324:383–385

    Google Scholar 

  35. Sawyer L, Papiz MZ, North ACT, Eliopoulos E (1985) Structure and function of bovine beta-lactoglobulin. Biochem Soc Trans 13:265–266

    Google Scholar 

  36. Adams JJ, Anderson BF, Norris GE, Creamer LK, Jameson GB (2006) Structure of bovine [beta]-lactoglobulin (variant A) at very low ionic strength. J Struct Biol 154:246–254

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by “the Eleventh Five-Year Plan” Science and Technology Research Program of China (2006BAD04A01 & 2006BAD04A12) and Jiangsu province (BE2008306-2), and Anhui Natural Science and Technology Program of Anhui province (10020303043 369 & KJ2009A039).

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Authors and Affiliations

  1. College of Life Sciences, Anhui Normal University, Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Wuhu, 241000, Anhui, China

    Fan Yang, Huiling Liu & Yafei Cai

  2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Fan Yang, Lian Li & Genlin Wang

Authors
  1. Fan Yang
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  2. Lian Li
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  3. Huiling Liu
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  4. Yafei Cai
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  5. Genlin Wang
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Corresponding authors

Correspondence to Yafei Cai or Genlin Wang.

Additional information

L. Li and H. Liu equally contributed to the study, and should be considered as first authors.

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Yang, F., Li, L., Liu, H. et al. Polymorphism in the exon 4 of β-lactoglobulin variant B precursor gene and its association with milk traits and protein structure in Chinese Holstein. Mol Biol Rep 39, 3957–3964 (2012). https://doi.org/10.1007/s11033-011-1175-6

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  • DOI: https://doi.org/10.1007/s11033-011-1175-6

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