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Development of single-locus DNA microsatellite markers using 5′anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia

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Abstract

Horseshoe crabs are said to be declining worldwide. However, there is still no published report on the status of horseshoe crabs in Malaysia. Thus, we report here eight informative microsatellite markers that were developed using the 5′-anchored ISSR-PCR enrichment procedure to diagnose the population genetic structure of the mangrove horseshoe crab, Carcinoscorpius rotundicauda from Peninsular Malaysia. This set of markers was tested on 127 samples and showed polymorphism in this species. Hence they should be useful in future essential population genetic studies of these living fossils in the Southeast Asian region.

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Acknowledgment

This work was supported by grant No. 05/01/07/0220RU (91220) from Universiti Putra Malaysia.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    A. B. Adibah, L. Pui Ling & S. G. Tan

  2. Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Q. Z. Faridah

  3. Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    A. Christianus

Authors
  1. A. B. Adibah
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  2. L. Pui Ling
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  3. S. G. Tan
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  4. Q. Z. Faridah
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  5. A. Christianus
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Corresponding author

Correspondence to S. G. Tan.

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Adibah, A.B., Ling, L.P., Tan, S.G. et al. Development of single-locus DNA microsatellite markers using 5′anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia. Mol Biol Rep 39, 3815–3820 (2012). https://doi.org/10.1007/s11033-011-1159-6

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  • DOI: https://doi.org/10.1007/s11033-011-1159-6

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