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Polymorphisms of myostatin gene (MSTN) in four goat breeds and their effects on Boer goat growth performance

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Abstract

Polymorphisms of myostatin (MSTN) gene were investigated as a candidate marker for goat growth in 687 individuals by gene sequencing and polymerase chain reaction restriction fragment length polymorphism methods. Three potential genotypes (AA, AB and BB) of 5 bp indel (1,256 TTTTA/−) in 5′UTR were detected in four breeds. The polymorphism (CC, CD and DD) of substitution (1,388 T/A) in exon 1 was only segregating in Boer. Genotype AB resulted in significant increases in body weights at birth (BW0), 90 (BW90) and 300 (BW300) days of age, and birth body length compared to BB (P < 0.05). Whilst genotype CD contributed to heavier BW0 and BW90, and larger birth body height (BH) compared to CC (P < 0.05). Individuals either with AB or CD genotype also had greater values in other body sizes, although no significant differences appeared (P > 0.05). When in combination, the combined genotype ABCD displayed positive impacts on better growth traits in BW0, BW90, BW300, BH and BCG (P < 0.05). These results indicate that these markers in myostatin (MSTN) are associated with Boer growth and may be useful for marker assisted selection.

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Acknowledgments

The authors wish to thank all the staff in Hubei Boer Goat Breeding Station and Shiyan Municipal Bureau of Animal Husbandry for their good work in the data collection. We also appreciate the valuable suggestion on this article given by Dr. Graham Plastow in University of Alberta. This work was financially supported by grants from the both National Natural Science Foundation of China (31001007) and Government Financial Support Project of China (2010BAD05B01).

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Authors and Affiliations

  1. China Education Ministry’s Key Laboratory in Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Chunyan Zhang, Yun Liu, Xiang Li & Liguo Yang

  2. Boer Goat Breeding Station, Yidu, 443300, China

    Deqing Xu

  3. Shiyan Municipal Bureau of Animal Husbandry, Shiyan, 442000, China

    Qunying Wen

  4. Department of Animal Physiology, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Wenmin Zhang

  5. Department of Agricultural Food and Nutrition Science, University of Alberta, Edmonton, T6G 2C8, Canada

    Chunyan Zhang

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  1. Chunyan Zhang
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  2. Yun Liu
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  3. Deqing Xu
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  4. Qunying Wen
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  5. Xiang Li
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  6. Wenmin Zhang
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  7. Liguo Yang
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Correspondence to Liguo Yang.

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Yun Liu--co-first author.

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Zhang, C., Liu, Y., Xu, D. et al. Polymorphisms of myostatin gene (MSTN) in four goat breeds and their effects on Boer goat growth performance. Mol Biol Rep 39, 3081–3087 (2012). https://doi.org/10.1007/s11033-011-1071-0

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  • DOI: https://doi.org/10.1007/s11033-011-1071-0

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