Skip to main content

Advertisement

SpringerLink
Log in

Characterization of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Artemisia annua

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Allene oxide synthase (AOS) is the first committed step in the biosynthetic pathway of Jasmonate. In this study, a full-length cDNA of AOS gene (named as AaAOS) was cloned from Artemisia annua. The gene was 1891 bp in size containing an open reading frame (1581 bp) encoding 526 amino acids. Comparative and bioinformatic analysis revealed that the deduced protein of AaAOS was highly homologous to AOSs from other plant species. Phylogenetic analysis indicated that the protein of AaAOS belonged to the dicotyledonous group, which was consistent with the category of A. annua. Southern blot analysis revealed that it was a low-copy gene. Quantitative Real-time PCR (qRT-PCR) analysis showed that AaAOS mRNA accumulated most abundantly in leaves and flowers. The qRT-PCR analysis revealed that MeJA, ABA and ethylene treatments significantly enhanced AaAOS transcript expression.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

AOS:

Allene oxide synthase

JAs:

Jasmonates

RACE:

Rapid amplification of cDNA ends

qRT-PCR:

Quantitative Real-time PCR

References

  1. Haynes RK (2006) From artemisinin to new artemisinin antimalarials: biosynthesis, extraction old and new derivatives, stereochemistry and medicinal chemistry requirements. Curr Top Med Chem 6:509–537

    Article  PubMed  CAS  Google Scholar 

  2. Weathers PJ, Elkholy S, Wobbe KK (2006) Artemisinin: the biosynthetic pathway and its regulation in Artemisia annua, a terpenoid-rich species. In Vitro Cell Dev Biol Plant 42:309–317

    Article  CAS  Google Scholar 

  3. Efferth T (2007) Antiplasmodial and antitumor activity of artemisinin from bench to bedside. Planta Med 73:299–309

    Article  PubMed  CAS  Google Scholar 

  4. Efferth T, Romero MR, Wolf DG, Stamminger T, Marin JJ, Marschall M (2008) The antiviral activities of artemisinin and artesunate. Clin Infect Dis 47:804–811

    Article  PubMed  CAS  Google Scholar 

  5. Baldi A, Dixit VK (2008) Yield enhancement strategies for artemisinin production by suspension cultures of Artemisia annua. Bioresour Technol 99:4609–4614

    Article  PubMed  CAS  Google Scholar 

  6. Wang HH, Ma CF, Li ZQ, Ma LQ, Wang H, Ye HC, Xu GW, Liu BY (2010) Effects of exogenous methyl jasmonate on artemisinin biosynthesis and secondary metabolites in Artemisia annua L. Ind Crop Prod 31:214–218

    Article  Google Scholar 

  7. Guo XX, Yang XQ, Yang RY, Zeng QP (2010) Salicylic acid and methyl jasmonate but not Rose Bengal enhance artemisinin production through invoking burst of endogenous singlet oxygen. Plant Sci 178:390–397

    Article  CAS  Google Scholar 

  8. Maes L, Van Nieuwerburgh FC, Zhang Y, Reed DW, Pollier J, Vande Casteele SR, Inzé D, Covello PS, Deforce DL, Goossens A (2010) Dissection of the phytohormonal regulation of trichome formation and biosynthesis of the antimalarial compound artemisinin in Artemisia annua plants. New Phytol. doi: 10.1111/j.1469-8137.2010.03466.x

  9. Avanci NC, Luche DD, Goldman GH, Goldman MH (2010) Jasmonates are phytohormones with multiple functions, including plant defense and reproduction. Genet Mol Res 9:484–505

    Article  PubMed  CAS  Google Scholar 

  10. Jiang KJ, Pi Y, Hou R, Zeng HN, Huang ZS, Zhang Z, Sun XF, Tang KX (2009) Molecular cloning and expression profiling of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Lonicera japonica. Mol Biol Rep 36:487–493

    Article  PubMed  CAS  Google Scholar 

  11. Laudert D, Pfannschmidt U, Lottspeich F, Holländer-Czytko H, Weiler EW (1996) Cloning, molecular and functional characterization of Arabidopsis thaliana allene oxide synthase (CYP74), the first enzyme of the octadecanoid pathway to jasmonates. Plant Mol Biol 31:323–335

    Article  PubMed  CAS  Google Scholar 

  12. Song WC, Brash AR (1991) Purification of an allene oxide synthase and identification of the enzyme as a cytochrome P-450. Science 253:781–784

    Article  PubMed  CAS  Google Scholar 

  13. Howe GA, Lee GI, Itoh A, Li L, DeRocher AE (2000) Cytochrome P450-dependent metabolism of oxylipins in tomato. Cloning and expression of allene oxide synthase and fatty acid hydroperoxide lyase. Plant Physiol 123:711–724

    Article  PubMed  CAS  Google Scholar 

  14. Maucher H, Hause B, Feussner I, Ziegler J, Wasternack C (2000) Allene oxide synthases of barley (Hordeum vulgare cv. Salome): tissue specific regulation in seedling development. Plant J 21:199–213

    Article  PubMed  CAS  Google Scholar 

  15. Agrawal GK, Rakwal R, Jwa NS, Han KS, Agrawal VP (2002) Molecular cloning and mRNA expression analysis of the first rice jasmonate biosynthetic pathway gene allene oxide synthase. Plant Physiol Biochem 40:771–782

    Article  CAS  Google Scholar 

  16. Kongrit D, Jisaka M, Iwanaga C, Yokomichi H, Katsube T, Nishimura K, Nagaya T, Yokota K (2007) Molecular cloning and functional expression of soybean allene oxide synthases. Biosci Biotechnol Biochem 71:491–498

    Article  PubMed  CAS  Google Scholar 

  17. Ishiga Y, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y (2003) Expression of allene oxide synthase and allene oxide cyclase in the interactions between pea and fungal pathogens. J Gen Plant Pathol 69:351–357

    Article  CAS  Google Scholar 

  18. Turner JG, Ellis C, Devoto A (2002) The jasmonate signal pathway. Plant Cell 14(Suppl):153–164

    Google Scholar 

  19. Comber C, Blanchet C, Geourjon C, Deleage G (2000) NPS@: network protein sequence analysis. Trends Biochem Sci 25:147–150

    Article  Google Scholar 

  20. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  21. Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245

    Article  PubMed  CAS  Google Scholar 

  22. Vick BA, Zimmerman DC (1987) Pathways of fatty acid hydroperoxide metabolism in spinach leaf chloroplasts. Plant Physiol 85:1073–1078

    Article  PubMed  CAS  Google Scholar 

  23. Froehlich JE, Itoh A, Howe GA (2001) Tomato allene oxide synthase and fatty acid hydroperoxide lyase, two cytochrome P450s involved in oxylipin metabolism, are targeted to different membranes of chloroplast envelope. Plant Physiol 125:306–317

    Article  PubMed  CAS  Google Scholar 

  24. Song WC, Funk CD, Brash AR (1993) Molecular cloning of an allene oxide synthase: a cytochrome P450 specialized for the metabolism of fatty acid hydroperoxides. Proc Natl Acad Sci USA 90:8519–8523

    Article  PubMed  CAS  Google Scholar 

  25. Emanuelsson O, Nielsen H, Von Heijne G (1999) ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci 8:978–984

    Article  PubMed  CAS  Google Scholar 

  26. Lee DS, Nioche P, Hamberg M, Raman CS (2008) Structural insights into the evolutionary paths of oxylipin biosynthetic enzymes. Nature 455:363–368

    Article  PubMed  CAS  Google Scholar 

  27. Laudert D, Weiler EW (1998) Allene oxide synthase: a major control point in Arabidopsis thaliana octadecanoid signalling. Plant J 15:675–684

    Article  PubMed  CAS  Google Scholar 

  28. Browse J (2009) Jasmonate passes muster: a receptor and targets for the defense hormone. Annu Rev Plant Biol 60:183–205

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was funded by China “863” Program, China “973” Program, Ministry of Education, Shanghai Science and Technology Committee and Shanghai Leading Academic Discipline Project (B209).

Author information

Authors and Affiliations

  1. Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Xu Lu, Fangyuan Zhang, Weimin Jiang, Xiuyan Lin, Yunfei Chen, Qian Shen, Tao Wang, Shaoyan Wu, Xiaofen Sun & Kexuan Tang

Authors
  1. Xu Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fangyuan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Weimin Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiuyan Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yunfei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qian Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Tao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Shaoyan Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Xiaofen Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Kexuan Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kexuan Tang.

Additional information

The novel nucleotide sequence data published here have been deposited in the EMBL/DDBJ/GenBank databases under accession number HQ533173.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lu, X., Zhang, F., Jiang, W. et al. Characterization of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Artemisia annua . Mol Biol Rep 39, 2267–2274 (2012). https://doi.org/10.1007/s11033-011-0976-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-011-0976-y

Keywords

Search

Navigation