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Thymidylate synthase and methionine synthase polymorphisms are not associated with susceptibility to childhood acute lymphoblastic leukemia in Kurdish population from Western Iran

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Abstract

In order to determine the influence of polymorphism in thymidylate synthase (TS 28-bp repeat) and methionine synthase (MS A2756G) genes on the susceptibility to acute lymphoblastic leukemia (ALL), 73 children with ALL and 128 age and sex matched unrelated healthy individuals from the Kermanshah Province of Iran were screened. The genotyping of TS 28-bp repeat and MS A2756G polymorphisms were performed by polymerase chain reaction (PCR) and PCR–RFLP, respectively. The frequency of TS 2R allele in patients and controls were 41.5 and 38%, respectively (Odds ratios (OR) = 1.13, 95%CI 0.73–1.74, P = 0.56). The allelic frequency of G allele of MS was higher (25%) in patients compared with healthy subjects (23%) (OR = 1.09, 95%CI 0.67–1.75, P = 0.71). Considering MS AA and TS 3R3R genotypes as reference indicated that individuals with MS GG + TS 2R2R genotypes have 1.3-fold increase in the risk of ALL (OR = 1.3, 95%CI 0.6–2.7, P = 0.5). Our results showed that neither TS 28-bp repeat nor MS A2756G polymorphisms are risk factors for susceptibility to ALL in Western Iran.

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Acknowledgments

This work was financially supported by a grant from Vice Chancellor for Research of Kermanshah University of Medical Sciences, Kermanshah, Iran.

Conflict of interest

The authors report no conflict of interest.

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Authors and Affiliations

  1. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Zohreh Rahimi, Zainab Ahmadian & Ziba Rahimi

  2. Department of Biochemistry, Medical Biology Research Center, Medical School, Daneshgah Avenue, 67148-69914, Kermanshah, Iran

    Zohreh Rahimi & Asad Vaisi-Raygani

  3. Department of Pharmacology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Zainab Ahmadian

  4. Department of Pediatrics Hematology Oncology, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Reza Akramipour

  5. Division of Neuroscience & Behavior, NIAAA, National Institutes of Health, Rockville, MD, USA

    Abbas Parsian

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  1. Zohreh Rahimi
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  2. Zainab Ahmadian
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  3. Reza Akramipour
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  4. Asad Vaisi-Raygani
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  5. Ziba Rahimi
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Correspondence to Zohreh Rahimi.

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Rahimi, Z., Ahmadian, Z., Akramipour, R. et al. Thymidylate synthase and methionine synthase polymorphisms are not associated with susceptibility to childhood acute lymphoblastic leukemia in Kurdish population from Western Iran. Mol Biol Rep 39, 2195–2200 (2012). https://doi.org/10.1007/s11033-011-0968-y

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  • DOI: https://doi.org/10.1007/s11033-011-0968-y

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