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Al toxicity leads to enhanced cell division and changed photosynthesis in Oryza rufipogon L.

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Abstract

Oryza rufipogon L. (O. rufipogon) or a common wild rice, showed considerable aluminum (Al) tolerance. In this study, we examined the physiologic and genetic response of wild rice short term and long term to Al toxicity, respectively. In the short term study, morin staining, DAPI staining and aniline blue staining were used to detect Al distribution, cell division and callose production in the roots of O. rufipogon. The results indicated cell division could be enhanced by Al within low concentration range. In the long term study, we chose Oryza sativa L (O. sativa) (the close sib of O. rufipogon) as a reference. It showed that O. rufipogon grew better than O. sativa when treated with Al of 1.4 mmol/l concentration and also experienced a short period of root growth stimulation. This study gave some basic data to explain the mechanisms Oryza rufipogon L. developed to deal with Al and lay a good foundation to further study. SSH (suppression subtractive hybridization) proved that transcripts of the small subunit of Rubisco and a Photosystem I P700 apoprotein were enhanced under long term Al treatment in wild rice. Further investigation via the assessment of the content of chlorophyll a, b indicated that the content of chlorophyll a, b in the leaves of O. rufipogon generally rose after Al treatment for 15 days. This indicated that intake of Al can affect photosynthesis of plant.

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Abbreviations

RRE:

Relative root elongation

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Acknowledgments

This study was supported by the National High Technology Research and Development Program of China (2008AA10Z116), the Ministry of Agriculture of China (2008ZX08009-001) and the Youth Science and Technology Phosphor Foundation of Shanghai (08QH14003), and partly by the Science and Technology Foundation of Shanghai (083391910400).

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Authors and Affiliations

  1. Institute of Genetics, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, 200433, China

    Yingping Cao, Yingying Han, Jinlei Shi, YaoFeng Wang, Wei Wang & Feng Ming

  2. Institute of Plant Biology, School of Life Science, Fudan University, Shanghai, 200433, People’s Republic of China

    Yingping Cao, Yuxia Lou, Yingying Han, Jinlei Shi, YaoFeng Wang, Wei Wang & Feng Ming

  3. College of Life and Environment Science, Shanghai Normal University, Shanghai, 200234, People’s Republic of China

    Yuxia Lou

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  1. Yingping Cao
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  2. Yuxia Lou
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  3. Yingying Han
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  4. Jinlei Shi
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  5. YaoFeng Wang
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  6. Wei Wang
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  7. Feng Ming
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Correspondence to Feng Ming.

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Yingping Cao and Yuxia Lou contributed equally to this work.

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Cao, Y., Lou, Y., Han, Y. et al. Al toxicity leads to enhanced cell division and changed photosynthesis in Oryza rufipogon L.. Mol Biol Rep 38, 4839–4846 (2011). https://doi.org/10.1007/s11033-010-0618-9

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  • DOI: https://doi.org/10.1007/s11033-010-0618-9

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