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Complete mitochondrial DNA sequences of the Nile tilapia (Oreochromis niloticus) and Blue tilapia (Oreochromis aureus): genome characterization and phylogeny applications

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Abstract

Cichlid fishes have played an important role in evolutionary biology and aquaculture industry. Nile tilapia (Oreochromis niloticus), blue tilapia (Oreochromis aureus) and Mozambique tilapia (Oreochromis mossambicus), the useful models in studying evolutionary biology within Cichlid fishes, are also mainly cultured species in aquaculture with great economic importance. In this paper, the complete nucleotide sequence of the mitochondrial genome for O. niloticus and O. aureus were determined and phylogenetic analyses from mitochondrial protein-coding genes were conducted to explore their phylogenetic relationship within Cichlids. The mitogenome is 16,625 bp for O. niloticus and 16,628 bp for O. aureus, containing the same gene order and an identical number of genes or regions with the other Cichlid fishes, including 13 protein-coding genes, two rRNA genes, 22 tRNA genes and one putative control region. Phylogenetic analyses using three different computational algorithms (maximum parsimony, maximum likelihood and Bayesian method) show O. niloticus and O. mossambicus are closely related, and O. aureus has remotely phylogenetic relationship from above two fishes.

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Acknowledgments

The authors are grateful to Jun Wang, Lu Zhou for their help with experiments assistance. This work was supported by the Tilapia Industry Special Program (hyhyzx07-044-01) and Tilapia Industry Technology System in China (mycytx-48) as well as the “Sustaining Ethical Aquaculture Trade” project funded by EU 7th Framework Program(Grant agreement no.: 222889).

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Authors and Affiliations

  1. Key Laboratory of Aquatic Genetic Resources and Utilization, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China

    Anyuan He, Liping Liu, Sifa Li & Chenghui Wang

  2. Guanxi Fisheries Institute, Nanling, 530021, Guanxi, China

    Yongju Luo

  3. Freshwater Fisheries Research Center, Chinese Academy of Fisheries Science, Wuxi, 214081, Jiangsu, China

    Hong Yang

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  1. Anyuan He
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  2. Yongju Luo
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  3. Hong Yang
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  4. Liping Liu
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  5. Sifa Li
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  6. Chenghui Wang
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Correspondence to Chenghui Wang.

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He, A., Luo, Y., Yang, H. et al. Complete mitochondrial DNA sequences of the Nile tilapia (Oreochromis niloticus) and Blue tilapia (Oreochromis aureus): genome characterization and phylogeny applications. Mol Biol Rep 38, 2015–2021 (2011). https://doi.org/10.1007/s11033-010-0324-7

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  • DOI: https://doi.org/10.1007/s11033-010-0324-7

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