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DLC-1 as a modulator of proliferation, apoptosis and migration in Burkitt’s lymphoma cells

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Abstract

Deleted in liver cancer-1(DLC-1) gene expression is frequently down-regulated or deleted in many types of human cancer. To evaluate whether DLC-1 could be a therapeutic target for non-Hodgkin lymphoma (NHL), we examined the expressions of DLC-1 in Burkitt’s lymphoma (BL) cell lines and tested the effects of DLC-1 on cellular growth and migration in BL cells. DLC-1 expression was not detectable in two human BL cell lines, Raji and Daudi, by reverse transcription-PCR. The transfer of DLC-1 into Raji and Daudi cell lines caused a significant inhibition in cell proliferation. This inhibitory effect on cell proliferation in BL cell lines was accompanied by induction of apoptosis. Furthermore, restoration of DLC-1 expression in BL cells had a significant inhibitory effect on migration. Our findings suggest that DLC-1 may play an important role in lymphoma by acting as a bona fide new tumor suppressor gene.

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Acknowledgments

We thank Dr. Ronggui Hu and Dr. Zhaoyun Zhang for critical reading and editing of the manuscript. The study was supported by the Science Foundation of Education Bureau of Zhejiang Province (Grant No. Y200804608) and the grant of Shanghai Municipal Health Bureau (Grant No. 2010079).

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Authors and Affiliations

  1. Ningbo College of Health Science, Ningbo, 315000, China

    Minhua Feng

  2. Department of Hematology, Huashan Hospital, Fudan University, Shanghai, 200040, China

    Bo Huang, Xiaoping Xu & Zi Chen

  3. Department of Pathology, Huashan Hospital, Fudan University, Shanghai, 200040, China

    Zunguo Du

Authors
  1. Minhua Feng
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  2. Bo Huang
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  3. Zunguo Du
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  4. Xiaoping Xu
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  5. Zi Chen
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Corresponding author

Correspondence to Zi Chen.

Additional information

Minhua Feng and Bo Huang contributed equally to this study.

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Feng, M., Huang, B., Du, Z. et al. DLC-1 as a modulator of proliferation, apoptosis and migration in Burkitt’s lymphoma cells. Mol Biol Rep 38, 1915–1920 (2011). https://doi.org/10.1007/s11033-010-0311-z

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  • DOI: https://doi.org/10.1007/s11033-010-0311-z

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