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DHA regulates lipogenesis and lipolysis genes in mice adipose and liver

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Abstract

Docosahexaenoic acid (DHA) is one kind of ω-3 polyunsaturated fatty acids (PUFAs) and plays an important role in lipid metabolism. In this research, mice were daily intragastric administrated with DHA for 3 weeks. Subcutaneous adipose tissue and liver were separated every week, RNA was extracted. Peroxisome proliferator-activated receptor (PPARγ), Sterol regulatory element binding protein-1c (SREBP-1c), Fatty acid synthetase (FAS), Hormone sensitive lipase (HSL) and triglyceride hydrolase TGH genes expression were detected by quantitative PCR. Data showed that, DHA up-regulated PPARγ, HSL and TGH in adipose tissue, but it had no effect on SREBP-1c and FAS expression. However, in liver there were some differences in regulating these genes. PPARγ, SREBP-1c and FAS were down-regulated, HSL was up-regulated and TGH had no change. These results indicated that DHA played different regulating roles in lipid metabolism in different tissues. In adipose tissue, DHA increased the expression of lipogenesis and lipolysis genes. In liver lipogenesis genes were decreased, but lipolysis genes were increased by DHA. In conclusion, DHA could reduce body fat mass through regulating lipogenesis and lipolysis genes.

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Acknowledgments

This work was supported by a grant from The National Nature Science Foundation of China (30871785), Program for New Century Excellent Talents in University of the Chinese Ministry of Education (NCET-06-0865) and the key Project of Chinese Ministry of Education (105167).

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  1. College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, China

    Chao Sun, Zhou-wen Wei & Yan Li

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  1. Chao Sun
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Correspondence to Chao Sun.

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Sun, C., Wei, Zw. & Li, Y. DHA regulates lipogenesis and lipolysis genes in mice adipose and liver. Mol Biol Rep 38, 731–737 (2011). https://doi.org/10.1007/s11033-010-0160-9

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