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Gene expression profiles of adipose tissue of high-fat diet-induced obese rats by cDNA microarrays

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Abstract

To better understand the molecular basis of dietary obesity, we examined adipose tissue genes differentially expressed in a well-characterized rat model of high-fat diet (HFD)-induced obesity using cDNA microarrays. Male Sprague–Dawley rats were fed either the HFD or the normal diet. Seven weeks later, the weights of obese models (362.92 ± 39.65 g) were significantly higher than those of normal control rats (315.22 ± 42.30 g, P < 0.01) and the wet weights of adipose tissue of rats fed with HFD (9.29 ± 5.14 g) were significantly higher than those of normal control rats (4.09 ± 2.69 g, P < 0.01), which confirmed the successful preparation of obese models. cDNA microarrays containing 9 216 genes/Ests were used to investigate gene expression of adipose tissue. Autoradiographic analysis showed that 532, 154, and 22 genes were differently expressed over 2-, 3-, and 5-fold, respectively. The analysis of gene expression profiles indicated that 276 genes were up-regulated and 432 genes were down-regulated in response to HFD-induced obesity. Different clusters of genes associated with lipid metabolism, extracellular matrix, signal transduction, cytoskeleton, cell apoptosis, etc., such as VLCS-H2, DGAT, ACADVL, PHYH, SCD, ACACA, ACS, MMP-2, MMP-15, CD38, CAMK2D, CACNA1F, CAPZA2, TMOD3, ARPC2, KNS2, TPM1, MAPK8, GADD45B, DAXX, TOK-1, PRKACA, STAT6, were concerned.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No 30973231, 30772364), the Natural Science Foundation of Jiangsu Province, China (No BK2007230), and the Foundation of Ministry of Education, China (No 20070312001).

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Authors and Affiliations

  1. Department of Newborn Infants, Nanjing Children’s Hospital of Nanjing Medical University, 210008, Nanjing, China

    Jie Qiu, Rui Cheng & Xiao-yu Zhou

  2. Department of Pediatrics, Nanjing Maternity and Child Health Hospital of Nanjing Medical University, 210004, Nanjing, China

    Jin-gai Zhu, Chun Zhu, Da-ni Qin, Chun-zhao Kou & Xi-rong Guo

  3. Institute of Pediatrics of Nanjing Medical University, 210029, Nanjing, China

    Jin-gai Zhu, Chun Zhu, Da-ni Qin, Chun-zhao Kou & Xi-rong Guo

Authors
  1. Jie Qiu
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  2. Rui Cheng
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  3. Xiao-yu Zhou
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  4. Jin-gai Zhu
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  5. Chun Zhu
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  6. Da-ni Qin
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  7. Chun-zhao Kou
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  8. Xi-rong Guo
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Correspondence to Xi-rong Guo.

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Jie Qiu and Rui Cheng contributed equally to this work.

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Qiu, J., Cheng, R., Zhou, Xy. et al. Gene expression profiles of adipose tissue of high-fat diet-induced obese rats by cDNA microarrays. Mol Biol Rep 37, 3691–3695 (2010). https://doi.org/10.1007/s11033-010-0021-6

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  • DOI: https://doi.org/10.1007/s11033-010-0021-6

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