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Identification of genes involved in stem rust resistance from wheat mutant D51 with the cDNA-AFLP technique

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Abstract

Wheat (Triticum aestivum L.) stem rust caused by Puccinia graminis f. sp. tritici is one of the main diseases of wheat worldwide. Wheat mutant line D51, which was derived from the highly susceptible cultivar L6239, shows resistance to the prevailing races 21C3CPH, 21C3CKH, and 21C3CTR of P. graminis f. sp. tritici in China. In this study, we used the cDNA-AFLP technology to identify the genes that are likely involved in the stem rust resistance. EcoRI/MseI selective primers were used to generate approximately 1920 DNA fragments. Seventy five differentially transcribed fragments (3.91%) were identified by comparing the samples of 21C3CPH infected D51 with infected L6239 or uninfected D51. Eleven amplified cDNA fragments were sequenced. Eight showed significant similarity to known genes, including TaLr1 (leaf rust resistance gene), wlm24 (wheat powdery mildew resistance gene), stress response genes and ESTs of environment stress of tall fescue. These identified genes are involved in plant defense response and stem rust resistance and need further research to determine their usefulness in breeding new resistance cultivars.

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Acknowledgements

We are grateful to Professor Chao Yuanyin, Yao Ping and Qiu Yongchun from Shenyang Agricultural University for their technical assistance in the inoculation and indentation of wheat stem rust. This research was financially supported by the Natural Science Foundation of Heilongjiang Province (2007–2009), China.

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Authors and Affiliations

  1. College of Life Science, Northeast Forestry University, 150040, Harbin, China

    Jing Yin

  2. Institute of Crop Breeding, Heilongjiang Academy of Agricultural Sciences, 150086, Harbin, China

    Jialei Xiao, Hongji Zhang, Yan Sun, Yanling Diao, Jinghua Huang, Qiang Guo & Dongjun Liu

  3. Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences, 150086, Harbin, China

    Guangjin Wang

  4. Northeast Agricultural University, 150030, Harbin, Heilongjiang, China

    Fengming Ma

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  1. Jing Yin
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  2. Guangjin Wang
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  10. Dongjun Liu
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Correspondence to Jing Yin or Guangjin Wang.

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Yin, J., Wang, G., Xiao, J. et al. Identification of genes involved in stem rust resistance from wheat mutant D51 with the cDNA-AFLP technique. Mol Biol Rep 37, 1111–1117 (2010). https://doi.org/10.1007/s11033-009-9870-2

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  • DOI: https://doi.org/10.1007/s11033-009-9870-2

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