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MicroRNA-17 post-transcriptionally regulates polycystic kidney disease-2 gene and promotes cell proliferation

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Abstract

To identify the possible microRNAs (miRNAs) which target the polycystic kidney disease-2 gene (PKD2), and clarify effects of the miRNAs on PKD2. We preliminarily used bioinformatics to analyze 3′UTR (3′untranslated regions) of PKD1 and PKD2 in order to predict the potential microRNAs targeted on them. Subsequently, the stable cell lines with overexpression of microRNA-17 (miR-17) were screened, and luciferase assay combined with the mutation 3′UTR of PKD2 were performed to verify PKD2 is the target of miR-17. Moreover, RT-PCR and Western Blotting were used to determine the post-transcriptionally regulation of PKD2 by miR-17. Finally, MTT cell assays allied with PKD2 rescued strategy were employed to evaluate cell proliferation effects. Our study firstly found that the 3′UTR of PKD2 was more conservation than that of PKD1, and microRNA-17 directly targets the 3′UTR of PKD2 and post-transcriptionally repress the expression of PKD2. Moreover, our findings also demonstrated that overexpression of miR-17 may promote cell proliferation via post-transcriptionally repression of PKD2 in HEK 293T. This suggested that microRNA might be a novel mechanism for cystogenesis as well as a potential therapeutic target for the cell proliferation of autosomal dominant polycystic kidney disease (ADPKD).

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Acknowledgements

This work was supported by a grant of the National Key Basic Research Program of China to Qin Zhou (2005CB522506) and a grant of the National Key Basic Research Program of China to academician Yuquan Wei (2004CB518800). Dr. Qin Zhou was a recipient of the Initial Foundation of M.O.E. for Returned Overseas Students (20071108-18-18) and a scholarship of the Creative Foundation of Sichuan University. The work was also supported by a grant of the S&T Bureau of Sichuan Province to Qin Zhou.

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Authors and Affiliations

  1. Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, 1# Keyuan the Fourth Road, The District of Hi&Tech, Chengdu, Sichuan, 610041, People’s Republic of China

    Huan Sun, Qing-Wei Li, Jian-Zhong Ai, Qiu-Tan Yang, Jing-Jing Duan, Guo-Hui Bian, Yan Xiao, Yi-Dong Wang, Zheng Zhang, Yu-Hang Liu, Rui-Zhi Tan, Yang Yang, Yu-Quan Wei & Qin Zhou

  2. Department of Dermatology, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, People’s Republic of China

    Xiao-Yan Lv

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  1. Huan Sun
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Correspondence to Qin Zhou.

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H. Sun and Q.-W. Li contributed equally to this work.

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Sun, H., Li, QW., Lv, XY. et al. MicroRNA-17 post-transcriptionally regulates polycystic kidney disease-2 gene and promotes cell proliferation. Mol Biol Rep 37, 2951–2958 (2010). https://doi.org/10.1007/s11033-009-9861-3

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  • DOI: https://doi.org/10.1007/s11033-009-9861-3

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