Skip to main content

Advertisement

SpringerLink
Log in

Isolation and expression analysis of a novel major latex-like protein (MLP151) gene from Panax ginseng

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

This is the first reports on isolation and expression analysis of a major latex-like protein (MLP151) gene in Panax ginseng C.A. Meyer. A full-length cDNA of MLP151 was 850 bp and contained a 456 bp open reading frame encoding a polypeptide of 151 amino acids. A theoretical pI value of MLP151 was 4.86 and calculated molecular weight was about 16.87 kDa. The MLP homolog proteins are found in various plants and the neighbor-joining analysis revealed that MLP151 has the closest distance with Sn-1 (bell pepper, MLP homolog gene). We analyzed the expression of MLP151 in different levels in various organs of ginseng and plantlet. In the result, the gene was low expressed in plantlet. We treated the ginseng plantlets with nine kinds of different stresses and analyzed the expression profile of MLP151. Transcript levels were significantly induced by stress treatment of light and mannitol, whereas transcript levels were drastically decreased in dark, H2O2, salicylic acid and wounding samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

MLP:

Major latex protein

ORF:

Open reading frame

RT-PCR:

Reverse transcriptase polymerase chain reaction

References

  1. Nessler CL, Allen RD, Galewsky S (1985) Identification and characterization of latex-specific proteins in opium poppy. Plant Physiol 79:499–504. doi:10.1104/pp.79.2.499

    Article  CAS  PubMed  Google Scholar 

  2. Nessler CL, Burnett RJ (1992) Organization of the major latex protein gene family in opium poppy. Plant Mol Biol 20:749–752. doi:10.1007/BF00046460

    Article  CAS  PubMed  Google Scholar 

  3. Nessler CL, Vonder Haar RA (1990) Cloning and expression analysis of DNA sequences for the major latex protein of opium poppy. Planta 180:487–491. doi:10.1007/BF02411445

    Article  CAS  Google Scholar 

  4. Ruperti B, Bonghi C, Ziliotto F, Pagni S, Rasori A, Varotto S, Tonutti P, Giovannoni JJ, Ramina A (2002) Characterization of a major latex protein (MLP) gene down-regulated by ethylene during peach fruitlet abscission. Plant Sci 163:265–272. doi:10.1016/S0168-9452(02)00094-8

    Article  CAS  Google Scholar 

  5. Stromvik MV, Sundararaman VP, Vodkin LO (1999) A novel promoter from soybean that is active in a complex developmental pattern with and without its proximal 650 base pairs. Plant Mol Biol 41:217–231. doi:10.1023/A:1006312228617

    Article  CAS  PubMed  Google Scholar 

  6. Nam YW, Tichit L, Leperlier M, Cuerq B, Marty I, Lelievre JM (1999) Isolation and characterization of mRNAs differentially expressed during ripening of wild strawberry (Fragaria vesca L.) fruits. Plant Mol Biol 39:629–636. doi:10.1023/A:1006179928312

    Article  CAS  PubMed  Google Scholar 

  7. Aggelis A, John I, Karvouni Z, Grierson D (1997) Characterization of two cDNA clones for mRNAs expressed during ripening of melon (Cucumis melo L.) fruits. Plant Mol Biol 33:313–322. doi:10.1023/A:1005701730598

    Article  CAS  PubMed  Google Scholar 

  8. Pozueta-Romero J, Klein M, Houlne G, Schantz ML, Meyer B, Schantz R (1995) Characterization of a family of genes encoding a fruit-specific wound-stimulated protein of bell pepper (Capsicum annuum): identification of a new family of transposable elements. Plant Mol Biol 28:1011–1025. doi:10.1007/BF00032663

    Article  CAS  PubMed  Google Scholar 

  9. Nessler CL (1994) Sequence analysis of two new members of the major latex protein gene family supports the triploid-hybrid origin of the opium poppy. Gene 139:207–209. doi:10.1016/0378-1119(94)90756-0

    Article  CAS  PubMed  Google Scholar 

  10. Nessler CL, Kurz WG, Pelcher LE (1990) Isolation and analysis of the major latex protein genes of opium poppy. Plant Mol Biol 15:951–953. doi:10.1007/BF00039436

    Article  CAS  PubMed  Google Scholar 

  11. Meeks-Wagner DR, Dennis ES, Van KTT, Peacock WJ (1989) Tobacco genes expressed during in vitro floral initiation and their expression during normal plant development. Plant Cell 1:25–35

    Article  CAS  PubMed  Google Scholar 

  12. Neale AD, Wahleithner JA, Lund M, Bonnett HT, Kelly A, Meeks-Wagner DR, Peacock WJ, Dennis ES (1990) Chitinase, beta-1, 3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation. Plant Cell 2:673–684

    Article  CAS  PubMed  Google Scholar 

  13. Osmark P, Boyle B, Brisson N (1998) Sequential and structural homology between intracellular pathogenesis-related proteins and a group of latex proteins. Plant Mol Biol 38:1243–1246. doi:10.1023/A:1006060224012

    Article  CAS  PubMed  Google Scholar 

  14. Bufe A, Spangfort MD, Kahlert H, Schlaak M, Becker WM (1996) The major birch pollen allergen, Bet v 1, shows ribonuclease activity. Planta 199:413–415. doi:10.1007/BF00195733

    Article  CAS  PubMed  Google Scholar 

  15. Flores T, Alape-Giron A, Flores-Diaz M, Flores HE (2002) Ocatin. A novel tuber storage protein from the andean tuber crop oca with antibacterial and antifungal activities. Plant Physiol 128:1291–1302. doi:10.1104/pp.010541

    Article  CAS  PubMed  Google Scholar 

  16. Moiseyev GP, Fedoreyeva LI, Zhuravlev YN, Yasnetskaya E, Jekel PA, Beintema JJ (1997) Primary structures of two ribonucleases from ginseng calluses. New members of the PR-10 family of intracellular pathogenesis-related plant proteins. FEBS Lett 407:207–210. doi:10.1016/S0014-5793(97)00337-2

    Article  CAS  PubMed  Google Scholar 

  17. Ernst E (2005) The efficacy of herbal medicine—an overview. Fundam Clin Pharmacol 19:405–409. doi:10.1111/j.1472-8206.2005.00335.x

    Article  CAS  PubMed  Google Scholar 

  18. Nam MH, Heo EJ, Kim JY, Kim SI, Kwon KH, Seo JB, Kwon O, Yoo JS, Park YM (2003) Proteome analysis of the responses of Panax ginseng C. A. Meyer leaves to high light: use of electrospray ionization quadrupole-time of flight mass spectrometry and expressed sequence tag data. Proteomics 3:2351–2367. doi:10.1002/pmic.200300509

    Article  CAS  PubMed  Google Scholar 

  19. Herrera-Rodriguez MB, Perez-Vicente R, Maldonado JM (2007) Expression of asparagine synthetase genes in sunflower (Helianthus annuus) under various environmental stresses. Plant Physiol Biochem 45:33–38. doi:10.1016/j.plaphy.2006.12.002

    Article  CAS  PubMed  Google Scholar 

  20. Nagashima A, Hanaoka M, Shikanai T, Fujiwara M, Kanamaru K, Takahashi H, Tanaka K (2004) The multiple-stress responsive plastid sigma factor, SIG5, directs activation of the psbD blue light-responsive promoter (BLRP) in Arabidopsis thaliana. Plant Cell Physiol 45:357–368. doi:10.1093/pcp/pch050

    Article  CAS  PubMed  Google Scholar 

  21. Malamy J, Carr JP, Klessig DF, Raskin I (1990) Salicylic acid: a likely endogenous signal in the resistance response of tobacco to viral infection. Science 250:1002–1004. doi:10.1126/science.250.4983.1002

    Article  CAS  PubMed  Google Scholar 

  22. Yalpani N, Leon J, Lawton MA, Raskin I (1993) Pathway of salicylic acid biosynthesis in healthy and virus-inoculated tobacco. Plant Physiol 103:315–321

    CAS  PubMed  Google Scholar 

  23. Radauer C, Breiteneder H (2007) Evolutionary biology of plant food allergens. J Allergy Clin Immunol 120:518–525. doi:10.1016/j.jaci.2007.07.024

    Article  CAS  PubMed  Google Scholar 

  24. Metraux JP, Signer H, Ryals J, Ward E, Wyss-Benz M, Gaudin J, Raschdorf K, Schmid E, Blum W, Inverardi B (1990) Increase in salicylic acid at the onset of systemic acquired resistance in cucumber. Science 250:1004–1006. doi:10.1126/science.250.4983.1004

    Article  CAS  PubMed  Google Scholar 

  25. Chen Z, Silva H, Klessig DF (1993) Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. Science 262:1883–1886. doi:10.1126/science.8266079

    Article  CAS  PubMed  Google Scholar 

  26. Prasad TK, Anderson MD, Martin BA, Stewart CR (1994) Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. Plant Cell 6:65–74

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by GRCMVP for Technology Development Program of Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.

Author information

Authors and Affiliations

  1. Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Kiheung-gu Yongin, Kyunggi-do, 446-701, South Korea

    Hua Sun, Myung-Kyum Kim, Rama Krishna Pulla, Yu-Jin Kim & Deok-Chun Yang

Authors
  1. Hua Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Myung-Kyum Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rama Krishna Pulla
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yu-Jin Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Deok-Chun Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Deok-Chun Yang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sun, H., Kim, MK., Pulla, R.K. et al. Isolation and expression analysis of a novel major latex-like protein (MLP151) gene from Panax ginseng . Mol Biol Rep 37, 2215–2222 (2010). https://doi.org/10.1007/s11033-009-9707-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-009-9707-z

Keywords

Search

Navigation