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Plasma homocysteine and DNA damage profiles in normal and obese subjects in the Pakistani population

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Abstract

Dependence of plasma total homocysteine (tHcy) and DNA damage profiles on melanodialdehyde (MDA), oxidative stress, liver function tests (LFT), and lipids was studied in non-obese and obese subjects in the Pakistani population. Development of obesity is influenced by both genetic, biochemical and environmental factors. Plasma homocysteine (Hcy) and DNA damage profiles play a pivotal role in its progression. We studied 160 obesity patients and 160 lean subjects. Leukocytes were evaluated for DNA damage by comet assay and blood plasma for biochemical properties using commercial kits. Plasma Hcy level and DNA damage were strongly correlated with triglycerides (P < 0.000), LDL-cholesterol (P < 0.001), systolic blood pressure (P < 0.001), cholesterol (P < 0.004), MDA (P < 0.004) and total oxygen stress (P < 0.004) in obese individuals. Both Hyc and DNA damage were negatively associated with total anti-oxidant response and globulin. Both Hcy profile and DNA damage may have a role in the endothelium damage even in the normal range and are related to triglycerides, ALT, MDA, TOS, HDL- and LDL-cholesterol in the Pakistani population.

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Acknowledgments

These studies were supported by Higher Education Commission, Punjab Government and University of Agriculture, Faisalabad. Ethical Committee is thanked for permission to carry out this work. Lab staff and Departmental statistician are thanked for their assistance.

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Authors and Affiliations

  1. Center for Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, 38040, Pakistan

    Shazia A. Bukhari

  2. National Institute for Biotechnology & Genetic Engineering, P.O. Box 577, Faisalabad, 38000, Pakistan

    Muhammad I. Rajoka

  3. Institute of Chemistry, University of the Punjab, Lahore, Pakistan

    Saeed A. Nagra

  4. Department of Physiology and Pharmacology, University of Agriculture, Faisalabad, 38040, Pakistan

    Zia Ur Rehman

Authors
  1. Shazia A. Bukhari
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  2. Muhammad I. Rajoka
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  3. Saeed A. Nagra
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  4. Zia Ur Rehman
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Correspondence to Muhammad I. Rajoka.

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Bukhari, S.A., Rajoka, M.I., Nagra, S.A. et al. Plasma homocysteine and DNA damage profiles in normal and obese subjects in the Pakistani population. Mol Biol Rep 37, 289–295 (2010). https://doi.org/10.1007/s11033-009-9686-0

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  • DOI: https://doi.org/10.1007/s11033-009-9686-0

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