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A single nucleotide polymorphism in the porcine cathepsin K (CTSK) gene is associated with back fat thickness and production traits in Italian Duroc pigs

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Abstract

Cathepsin K (CTSK) was selected as a candidate gene for fat deposition in pigs because recently, in human and mouse, it was shown that this lysosomal proteinase is an obesity marker. A single nucleotide polymorphism (SNP) was identified in intron 4 of the porcine CTSK gene (g.15G>A; FM209043). Allele frequencies of this polymorphism were analysed in seven pig breeds. Radiation hybrid mapping confirmed the localization of CTSK to porcine chromosome 4, close to the FAT1 QTL region. Three populations of pigs (one Italian Large White and two Italian Duroc groups of pigs) were selected for association analysis. In the Italian Large White breed the g.15G>A SNP was not informative. Association analysis including all Italian Duroc pigs showed that the CTSK marker was associated with back fat thickness and lean cuts (P < 0.01), and average daily gain and feed:gain ratio (P < 0.05) estimated breeding values.

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Acknowledgements

We thank Dr. M. Yerle (INRA) for providing the INRA-Minnesota 7000 rads RH panel and ANAS for providing samples and data. This work was supported by University of Bologna (FAGenomicH project) and MiPAAF (Selmol project) funds.

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Authors and Affiliations

  1. DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Via F.lli Rosselli 107, 42100, Reggio Emilia, Italy

    Luca Fontanesi, Emilio Scotti, Stefania Dall’Olio, Roberta Davoli & Vincenzo Russo

  2. Associazione Nazionale Allevatori Suini (ANAS), Via Lazzaro Spallanzani 4/6, 00161, Rome, Italy

    Luca Buttazzoni

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  1. Luca Fontanesi
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  2. Emilio Scotti
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  3. Luca Buttazzoni
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  4. Stefania Dall’Olio
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  5. Roberta Davoli
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  6. Vincenzo Russo
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Correspondence to Luca Fontanesi.

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Fontanesi, L., Scotti, E., Buttazzoni, L. et al. A single nucleotide polymorphism in the porcine cathepsin K (CTSK) gene is associated with back fat thickness and production traits in Italian Duroc pigs. Mol Biol Rep 37, 491–495 (2010). https://doi.org/10.1007/s11033-009-9678-0

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