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Gene expression profiles in the PC-3 human prostate cancer cells induced by NKX3.1

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Abstract

NKX3.1, a prostate-specific gene, plays an important role in prostate development and carcinogenesis. However, its precise function has not been established. In present study, we transfected the NKX3.1 eukaryotic expression plasmid (pcDNA3.1-NKX3.1) into human prostate cancer cells PC-3, which lack of NKX3.1 expression, and established stable transfectants. Then, we investigated the influence of NKX3.1 on the cell growth, cell migration and colony formation efficiency. The results showed that restoration of NKX3.1 expression inhibited proliferation and invasion activities of PC-3 cells. Further, a cDNA microarray containing 22,000 human genes was used to identify the gene expression differences. The results showed that there were 1,953 genes showing more than a two-fold difference in expression. Subsequent ontological analysis revealed that a large proportion of the classified genes were related to cell growth, cell signal and cell invasion. Finally, the expression of Caspase-3, Bcl-2, P27, Cdk6 and AMACR, randomly selected genes from microarray data, was validated by RT-PCR and western blot. Collectively, our results first analyzed the gene expression profile in PC-3 cells induced by NKX3.1 and indicated that NKX3.1 might exert its function by regulating the expression of relative genes.

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Acknowledgments

This study was supported by the Natural Science Foundation of Shandong Province (No.Y2007C96 and No.Y2005C03) and the National Natural Science Foundation of China (No. 30870732 and No. 30670581)

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Authors and Affiliations

  1. Institution of Biochemistry and Molecular Biology, Medical School of Shandong University, 44 Wenhua West Road, 250012, Jinan, China

    Pengju Zhang, Wenwen Liu, Ju Zhang, Hengyun Guan, Weiwen Chen, Xing Cui & Anli Jiang

  2. Shandong Provincial Hospital, 250021, Jinan, China

    Wenwen Liu

  3. PET-CT Center, Shandong Provincial Hospital, 250021, Jinan, China

    Qingwei Liu

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  1. Pengju Zhang
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  2. Wenwen Liu
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  3. Ju Zhang
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  4. Hengyun Guan
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  7. Qingwei Liu
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  8. Anli Jiang
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Correspondence to Qingwei Liu or Anli Jiang.

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Zhang, P., Liu, W., Zhang, J. et al. Gene expression profiles in the PC-3 human prostate cancer cells induced by NKX3.1. Mol Biol Rep 37, 1505–1512 (2010). https://doi.org/10.1007/s11033-009-9549-8

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  • DOI: https://doi.org/10.1007/s11033-009-9549-8

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