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Molecular characterization, expression profile and association analysis with fat deposition traits of the porcine APOM gene

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Abstract

Apolipoprotein M (APOM), a novel apolipoprotein presented mostly in high-density lipoprotein (HDL) in plasma, is involved in lipid and lipoprotein metabolism. Through comparative mapping, we have mapped this gene to SSC7 p1.1 in which many QTLs affecting fat deposition traits have been reported. As a candidate gene for fat deposition traits, in this study, we obtained the 742-bp mRNA sequence of porcine APOM including the full coding region and encoding a protein of 188 amino acids. The sequence was deposited into the GenBank under the accession no. DQ329240. Semi-quantitative RT-PCR results showed that the porcine APOM gene is expressed predominantly in liver and kidney tissue. The genomic sequence of this gene which contains six exons and five introns, is 3,621 bp in length (DQ272488). Bioinformatic analysis of the 5′ regulatory region has revealed that classical TATA-box element and species conserved Hepatocyte nuclear factor-1a (HNF-1α) biding site were represented in this region. A G2289C single nucleotide polymorphism (SNP) in the intron 2 of porcine APOM gene detected as an Eco130I PCR–restriction fragment length polymorphism (PCR–RFLP) showed allele frequency differences among three purebreds. Association of the genotypes with fat deposition traits showed that different genotypes of porcine APOM gene were significantly associated with leaf fat weight (P < 0.05), backfat thickness at shoulder (P < 0.05), backfat thickness at thorax-waist (P < 0.05), backfat thickness at buttock (P < 0.01) and average backfat thickness over shoulder, thorax-waist and buttock (P < 0.01).

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References

  1. Xu N, Dahlback B (1999) A novel human apolipoprotein (apoM). J Biol Chem 274:31286–31290. doi:10.1074/jbc.274.44.31286

    Article  CAS  PubMed  Google Scholar 

  2. Luo G, Zhang X, Nilsson-Ehle P, Xu N (2004) Apolipoprotein M. Lipids Health Dis 3:21. doi:10.1186/1476-511X-3-21

    Article  PubMed  CAS  Google Scholar 

  3. Xu N, Ahren B, Nilsson-Ehle P (2004) Correlation of apolipoprotein M with leptin and cholesterol in normal and obese subjects. J Nutr Biochem 15:579–582. doi:10.1016/j.jnutbio.2004.03.001

    Article  CAS  PubMed  Google Scholar 

  4. Wolfrum C, Poy MN, Stoffel M (2005) Apolipoprotein M is required for prebeta-HDL formation and cholesterol efflux to HDL and protects against atherosclerosis. Nat Med 11:418–422. doi:10.1038/nm1211

    Article  CAS  PubMed  Google Scholar 

  5. Richter S, Shih DQ, Pearson ER, Wolfrum C, Fajans SS, Hattersley AT, Stoffel M (2003) Regulation of apolipoprotein M gene expression by MODY3 gene hepatocyte nuclear factor-1alpha: haploinsufficiency is associated with reduced serum apolipoprotein M levels. Diabetes 52:2989–2995. doi:10.2337/diabetes.52.12.2989

    Article  CAS  PubMed  Google Scholar 

  6. Xu N, Nilsson-Ehle P, Hurtig M, Ahren B (2004) Both leptin and leptin-receptor are essential for apolipoprotein M expression in vivo. Biochem Biophys Res Commun 321:916–921. doi:10.1016/j.bbrc.2004.06.180

    Article  CAS  PubMed  Google Scholar 

  7. Luo G, Hurtig M, Zhang X, Nilsson-Ehle P, Xu N (2005) Leptin inhibits apolipoprotein M transcription and secretion in human hepatoma cell line, HepG2 cells. Biochim Biophys Acta 1734:198–202

    CAS  PubMed  Google Scholar 

  8. Xu N, Zhang XY, Dong X, Ekstrom U, Ye Q, Nilsson-Ehle P (2002) Effects of platelet-activating factor, tumor necrosis factor, and interleukin-1alpha on the expression of apolipoprotein M in HepG2 cells. Biochem Biophys Res Commun 292:944–950. doi:10.1006/bbrc.2002.6755

    Article  CAS  PubMed  Google Scholar 

  9. Xu N, Hurtig M, Zhang XY, Ye Q, Nilsson-Ehle P (2004) Transforming growth factor-beta down-regulates apolipoprotein M in HepG2 cells. Biochim Biophys Acta 1683:33–37

    CAS  PubMed  Google Scholar 

  10. Xu N, Ahren B, Jiang J, Nilsson-Ehle P (2006) Down-regulation of apolipoprotein M expression is mediated by phosphatidylinositol 3-kinase in HepG2 cells. Biochim Biophys Acta 1761:256–260

    CAS  PubMed  Google Scholar 

  11. Zhang XY, Zhu ZJ, Luo GH, Zheng L, Nilsson-Ehle P, Xu N (2008) Liver X receptor agonist downregulates hepatic apoM expression in vivo and in vitro. Biochem Biophys Res Commun 371:114–117. doi:10.1016/j.bbrc.2008.04.017

    Article  CAS  PubMed  Google Scholar 

  12. Barbosa A, Demeure O, Urien C, Milan D, Chardon P, Renard C (2004) A physical map of large segments of pig chromosome 7q11–q14: comparative analysis with human chromosome 6p21. Mamm Genome 15:982–995. doi:10.1007/s00335-004-3008-6

    Article  CAS  PubMed  Google Scholar 

  13. Genêt C, Renard C, Cabau C, Rogel-Gaillard C, Gellin J, Milan D (2001) In the QTL region surrounding porcine MHC, gene order is conserved with human genome. Mamm Genome 12:246–249. doi:10.1007/s003350010261

    Article  PubMed  Google Scholar 

  14. Tanaka M, Matsumoto T, Yanai S et al (2003) Conservation of the syntenies between porcine chromosome 7 and human chromosomes 6, 14 and 15 demonstrated by radiation hybrid mapping and linkage analysis. Anim Genet 34:255–263. doi:10.1046/j.1365-2052.2003.00999.x

    Article  CAS  PubMed  Google Scholar 

  15. Rattink AP, de Koning DJ, Faivre M, Harlizius B, van Arendonk JA, Groenen MA (2000) Fine mapping and imprinting analysis for fatness trait QTLs in pigs. Mamm Genome 11:656–661. doi:10.1007/s003350010117

    Article  CAS  PubMed  Google Scholar 

  16. Rattink AP, De Koning DJ, Faivre M, Harlizius B, van Arendonk JA, Groenen MA (2000) Fine mapping and imprinting analysis for fatness trait QTLs in pigs. Mamm Genome 11(8):656–661. doi:10.1007/s003350010117

    Article  CAS  PubMed  Google Scholar 

  17. Sanchez MP, Riquet J, Iannuccelli N, Gogue J, Billon Y, Demeure O, Caritez JC, Burgaud G, Feve K, Bonnet M, Pery C (2006) Effects of quantitative trait loci on chromosomes 1, 2, 4, and 7 on growth carcass, and meat quality traits in backcross Meishan × Large White pigs. J Anim Sci 84:526–537

    CAS  PubMed  Google Scholar 

  18. Bidanel JP, Milan D, lannuccelli N, Amigues Y, Boscher MY (2001) Detection of quantitative trait loci for growth and fatness in pigs. Genet Sel Evol 33:289–309. doi:10.1051/gse:2001120

    Article  CAS  PubMed  Google Scholar 

  19. Dai LH, Xiong YZ, Deng CY, Jiang SW, Zuo B, Zheng R, Li FE, Lei MG (2006) Association of the A–G polymorphism in porcine adiponectin gene with fat deposition and carcass traits. Asian-australas J Anim Sci 19:779–783

    CAS  Google Scholar 

  20. Xiong YZ, Deng CY (1999) Principle and method of swine testing. Chinese Agriculture Press, Beijing

    Google Scholar 

  21. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  22. Liu BH (1998) Statistical genomics: linkage, mapping. and QTL Analysis. CRC Press, LLC, Boca Raton, pp 404–409

    Google Scholar 

  23. Zhang XY, Dong X, Zheng L, Luo GH, Liu YH, Ekstrom U, Nilsson-Ehle P, Ye Q, Xu N (2003) Specific tissue expression and cellular localization of human apolipoprotein M as determined by in situ hybridization. Acta Histochem 105:67–72. doi:10.1078/0065-1281-00687

    Article  CAS  PubMed  Google Scholar 

  24. Rabin M, Fries R, Singer D, Ruddle FH (1985) Assignment of the porcine major histocompatibility complex to chromosome 7 by in situ hybridisation. Cytogenet Cell Genet 39:206–209. doi:10.1159/000132136

    Article  CAS  PubMed  Google Scholar 

  25. Echard G, Yerle M, Gellin J, Dalens M, Gillois M (1986) Assignment of the major histocompatibility complex to the p14–q12 region of chromosome 7 in the pig by in situ hybridisation. Cytogenet Cell Genet 41:126–128. doi:10.1159/000132215

    Article  CAS  PubMed  Google Scholar 

  26. Sarmiento UM, Sarmiento JI, Lunney JK, Rishi S (1993) Mapping of the porcine SLA class I gene (PD1A) and the associated repetitive element (C11) by fluorescence in situ hybridisation. Mamm Genome 4:64–65. doi:10.1007/BF00364669

    Article  CAS  PubMed  Google Scholar 

  27. Smith TPL, Rohrer GA, Alexander LJ, Troyer DL, Kirby-Dobbels KR, Janzen MA, Cornwell DL, Louis C, Schook LB, Beattie CW (1995) Directed integration of the physical and genetic linkage maps of swine chromosome 7 reveals that SLA spans the centromere. Genome Res 5:259–271. doi:10.1101/gr.5.3.259

    Article  CAS  PubMed  Google Scholar 

  28. Davoli R, Fontanesi L, Zambonelli P, Bigi D, Gellin J, Yerle M, Milc J, Braglia S, Cenci V, Cagnazzo M, Russo V (2002) Isolation of porcine expressed sequence tags for the construction of a first genomic transcript map of the skeletal muscle in pig. Anim Genet 33:3–18. doi:10.1046/j.1365-2052.2002.00800.x

    Article  CAS  PubMed  Google Scholar 

  29. Chardon P, Nunes M, Dezeure F, Andres-Cara D, Vaiman M (1991) Mapping and genetic organisation of the TNF genes in the swine MHC. Immunogenetics 34:257–260. doi:10.1007/BF00215262

    Article  CAS  PubMed  Google Scholar 

  30. Solinas S, Pauli U, Kuhnert P, Peterhans E, Fries R (1992) Assignment of the porcine tumor necrosis factor alpha and beta genes to the chromosome region 7p11–q11 by in situ hybridisation. Anim Genet 23:267–271

    Article  CAS  PubMed  Google Scholar 

  31. Nunes M, Yerle M, Dezeure F, Gellin J, Chardon P, Vaiman M (1993) Isolation of four HSP70 genes in the pig and localization on chromosomes 7 and 14. Mamm Genome 4:247–251. doi:10.1007/BF00417430

    Article  CAS  PubMed  Google Scholar 

  32. Goureau A, Yerle M, Schmitz A, Riquet J, Milan D (1996) Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36:252–262. doi:10.1006/geno.1996.0460

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This study was funded by the National Natural Science Foundation of China (No. 30571331) and the National “973” Program of P. R. China (2006CB102102).

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Authors and Affiliations

  1. Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, 430070, Wuhan, China

    Gang Pan, Yayuan Fu, Bo Zuo, Zhuqing Ren, Dequan Xu, Minggang Lei, Rong Zheng & Yuan-Zhu Xiong

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  1. Gang Pan
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Correspondence to Yuan-Zhu Xiong.

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Pan, G., Fu, Y., Zuo, B. et al. Molecular characterization, expression profile and association analysis with fat deposition traits of the porcine APOM gene. Mol Biol Rep 37, 1363–1371 (2010). https://doi.org/10.1007/s11033-009-9518-2

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