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Another formula for calculating the gene change rate in real-time RT-PCR

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Abstract

Relative quantification real-time PCR has become a routine method in molecular biology research to study the small amount gene expression. There are a few mathematical models for relative quantification data analysis in real-time PCR, and the formula 2−∆∆CT is one of the most frequently used method. In this paper, we are to present another equation which directly calculates the change rate (R c) of gene expression, and give a example to compare the application of this model with 2−∆∆CT.

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Acknowledgments

The authors would like to thank Dongming Zhou M.D. from University of Pennsylvania (USA) for critically reading the manuscript.

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  1. Shujian Chang

    Present address: Department of Oncology, The Fourth Affiliated Hospital, Soochow University, 214062, Wuxi, China

Authors and Affiliations

  1. Department of Gastroenterology, The First Affiliated Hospital, Soochow University, 215006, Suzhou, China

    Shujian Chang & Weichang Chen

  2. Cell and Molecular Biology Institute, College of Medicine, Soochow University, 215132, Suzhou, China

    Jicheng Yang

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  1. Shujian Chang
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  2. Weichang Chen
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Correspondence to Weichang Chen.

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Chang, S., Chen, W. & Yang, J. Another formula for calculating the gene change rate in real-time RT-PCR. Mol Biol Rep 36, 2165–2168 (2009). https://doi.org/10.1007/s11033-008-9430-1

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  • DOI: https://doi.org/10.1007/s11033-008-9430-1

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