Skip to main content
SpringerLink
Log in

Genetic variations in chemoattractant receptor expressed on Th2 cells (CRTH2) is associated with asthma susceptibility in Chinese children

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

We investigated the allele and genotype frequencies of two common CRTH2 single nucleotide polymorphisms (SNPs) [G1544C and A1651G (rs 545659)] in the 3′-untranslated region and the relationship between these SNPs and serum IL-13 levels in Chinese children patients with asthma. For G1544C and A1651G SNPs, there were significant differences in allele and genotype frequencies between asthma patients and controls. Haplotype analysis yielded additional evidence of linkage disequilibrium for the 1544G–1651G haplotype (P < 0.01). Moreover, serum IL-13 levels were significantly different among genotypes in G1544C, A1651G SNPs. These results suggest that SNPs of G1544C and A1651G might be act as susceptibility genetic factors of asthma.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CRTH2:

Chemoattractant receptor expressed on Th2 cells

SNPs:

Single nucleotide polymorphisms

PBMCs:

Peripheral blood mononuclear cells

PDG2:

Prostaglandin D2

References

  1. Scirica CV, Celedón JC (2007) Genetics of asthma: potential implications for reducing asthma disparities. Chest 132(5 Suppl):770S–781S. doi:10.1378/chest.07-1905

    Article  PubMed  CAS  Google Scholar 

  2. Blumenthal MN, Ober C, Beaty TH, Bleecker ER et al (2004) Genome scan for loci linked to mite sensitivity: the Collaborative Study on the Genetics of Asthma (CSGA). Genes Immun 5(3):226–231. doi:10.1038/sj.gene.6364063

    Article  PubMed  CAS  Google Scholar 

  3. Liang KY, Hsu FC, Beaty TH (2003) Multipoint linkage disequilibrium mapping for complex diseases. Genet Epidemiol 25(4):285–292. doi:10.1002/gepi.10271

    Article  PubMed  Google Scholar 

  4. Huang SK, Mathias RA, Ehrlich E et al (2003) Evidence for asthma susceptibility genes on chromosome 11 in an African-American population. Hum Genet 113(1):71–75

    PubMed  CAS  Google Scholar 

  5. Mathias RA, Gao P, Goldstein JL et al (2006) A graphical assessment of p-values from sliding window haplotype tests of association to identify asthma susceptibility loci on chromosome 11q. BMC Genet 7:38–48. doi:10.1186/1471-2156-7-38

    Article  PubMed  Google Scholar 

  6. Ulven T, Kostenis E (2006) Targeting the prostaglandin D2 receptors DP and CRTH2 for treatment of inflammation. Curr Top Med Chem 6(13):1427–1444

    PubMed  CAS  Google Scholar 

  7. Ly TW, Bacon KB (2005) Small-molecule CRTH2 antagonists for the treatment of allergic inflammation: an overview. Expert Opin Investig Drugs 14(7):769–773. doi:10.1517/13543784.14.7.769

    Article  PubMed  CAS  Google Scholar 

  8. Huang JL, Gao PS, Mathias RA et al (2004) Sequence variants of the gene encoding chemoattractant receptor expressed on Th2 cells (CRTH2) are associated with asthma and differentially influence mRNA stability. Hum Mol Genet 13(21):2691–2697. doi:10.1093/hmg/ddh279

    Article  PubMed  CAS  Google Scholar 

  9. Maeda Y, Hizawa N, Takahashi D et al (2007) Genetic impact of functional single nucleotide polymorphisms in the 3′-UTR region of the chemoattractant receptor expressed on Th2 cells (CRTH2) gene on asthma and atopy in a Japanese population. Int Arch Allergy Immunol 142(1):51–58. doi:10.1159/000095998

    Article  PubMed  CAS  Google Scholar 

  10. Huang JL (2005) Asthma severity and genetics in Taiwan. J Microbiol Immunol Infect 38(3):158–163

    PubMed  CAS  Google Scholar 

  11. Wills-Karp M, Chiaramonte M (2003) Interleukin-13 in asthma. Curr Opin Pulm Med 9:21–27. doi:10.1097/00063198-200301000-00004

    Article  PubMed  CAS  Google Scholar 

  12. Zhu Z, Homer RJ, Wang Z et al (1999) Pulmonary expression of interleukin-13 causes inflammation, mucus hypersecretion, subepithelial fibrosis, physiologic abnormalities, and eotaxin production. J Clin Invest 103(6):779–788. doi:10.1172/JCI5909

    Article  PubMed  CAS  Google Scholar 

  13. Joshi BH, Hogaboam C, Dover P et al (2006) Role of interleukin-13 in cancer, pulmonary fibrosis, and other T(H)2-type diseases. Vitam Horm 74:479–504. doi:10.1016/S0083-6729(06)74019-5

    Article  PubMed  CAS  Google Scholar 

  14. Izuhara K, Arima K, Kanaji S et al (2006) IL-13: a promising therapeutic target for bronchial asthma. Curr Med Chem 13(19):2291–2298. doi:10.2174/092986706777935140

    Article  PubMed  CAS  Google Scholar 

  15. Wills-Karp M (2004) Interleukin-13 in asthma pathogenesis. Immunol Rev 202:175–190. doi:10.1111/j.0105-2896.2004.00215.x

    Article  PubMed  CAS  Google Scholar 

  16. O’Byrne PM, Inman MD, Adelroth E (2004) Reassessing the Th2 cytokine basis of asthma. Trends Pharmacol Sci 25(5):244–248. doi:10.1016/j.tips.2004.03.008

    Article  PubMed  Google Scholar 

  17. Corry DB, Kheradmand F (2002) Biology and therapeutic potential of the interleukin-4/interleukin-13 signaling pathway in asthma. Am J Respir Med 1(3):185–193

    PubMed  CAS  Google Scholar 

  18. Chiba T, Kanda A, Ueki S et al (2007) Possible novel receptor for PGD2 on human bronchial epithelial cells. Int Arch Allergy Immunol 143(Suppl 1):23–27. doi:10.1159/000101400

    Article  PubMed  CAS  Google Scholar 

  19. Mathiesen JM, Ulven T, Martini L et al (2005) Identification of indole derivatives exclusively interfering with a G protein-independent signaling pathway of the prostaglandin D2 receptor CRTH2. Mol Pharmacol 68(2):393–402

    PubMed  CAS  Google Scholar 

  20. Hirai H, Tanaka K, Yoshie O et al (2001) Prostaglandin D2 selectively induces chemotaxis in T helper type 2 cells, eosinophils, and basophils via seven-transmembrane receptor CRTH2. J Exp Med 193(2):255–261. doi:10.1084/jem.193.2.255

    Article  PubMed  CAS  Google Scholar 

  21. Spik I, Brénuchon C, Angéli V et al (2005) Activation of the prostaglandin D2 receptor DP2/CRTH2 increases allergic inflammation in mouse. J Immunol 174(6):3703–3708

    PubMed  CAS  Google Scholar 

  22. Nath P, Yee Leung S, Williams AS et al (2007) Complete inhibition of allergic airway inflammation and remodelling in quadruple IL-4/5/9/13−/− mice. Clin Exp Allergy 37(10):1427–1435

    PubMed  CAS  Google Scholar 

  23. Ly NP, Li Y, Sredl DL et al (2005) Elevated allergen-induced IL-13 secretion predicts IgE elevation in children ages 2–5 years. J Clin Immunol 25(4):314–320. doi:10.1007/s10875-005-4699-5

    Article  PubMed  CAS  Google Scholar 

  24. Hoshino A, Tsuji T, Matsuzaki J et al (2004) STAT6-mediated signaling in Th2-dependent allergic asthma: critical role for the development of eosinophilia, airway hyper-responsiveness and mucus hypersecretion, distinct from its role in Th2 differentiation. Int Immunol 16(10):1497–1505. doi:10.1093/intimm/dxh151

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Clinical Laboratory, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China

    Jinhui Wang

  2. Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150001, China

    Yongchen Xu, Hongyan Liang & Xiaofeng Jiang

  3. Department of Blood Transfusion, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China

    Hong Zhao

  4. Department of Oncology, The Third Affiliated Hospital of Harbin Medical University, Harbin, 150080, China

    Hong Sui

Authors
  1. Jinhui Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yongchen Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hong Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hong Sui
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hongyan Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiaofeng Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiaofeng Jiang.

Additional information

Jinhui Wang and Yongchen Xu should be regarded as joint First Authors.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, J., Xu, Y., Zhao, H. et al. Genetic variations in chemoattractant receptor expressed on Th2 cells (CRTH2) is associated with asthma susceptibility in Chinese children. Mol Biol Rep 36, 1549–1553 (2009). https://doi.org/10.1007/s11033-008-9349-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-008-9349-6

Keywords

Search

Navigation