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Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri

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Abstract

Peroxiredoxin V (PRX V) is known as an atypical 2-cysteine peroxiredoxin that protects the organisms against various oxidative stresses and functions in signal transduction. The cDNA of a PRX V gene (designated as CfPRX) was cloned from scallop Chlamys farreri. The full-length sequence of CfPRX cDNA was of 2,179 bp with a 564 bp open reading frame encoding a peptide of 187 amino acids. Sequence comparison showed that CfPRX shared higher identities with PRX Vs than that with other isoforms of PRX, indicating CfPRX was a member of the PRX V family. Fluorescent real-time quantitative PCR analysis revealed the presence of CfPRX transcripts in gill filaments, adductor muscle, heart, gonad, kidney and hemocytes, and the stimulation of Listonella anguillarum significantly (P < 0.01) enhanced the mRNA expression of CfPRX in hemocyte. These results indicated that CfPRX was a constitutive and inducible acute-phase protein which was involved in the immune resistance to L. anguillarum stimulation.

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Acknowledgements

The authors are grateful to Mr. Chaohua Dong, Yunchao Gai, Miss Huan Zhang and other laboratory members for technical advice and helpful discussions. This research was supported by 973 National Key Fundamental Research Program (No. 2006CB101806) and 863 High Technology Project (No. 2006AA10A402) from the Chinese Ministry of Science and Technology, and a grant (No.30730070) from NSFC to Prof. Linsheng Song.

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  1. Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd, Qingdao, 266071, Shandong, China

    Ming Cong, Duojiao Ni, Linsheng Song, Lingling Wang, Jianmin Zhao, Limei Qiu & Ling Li

  2. Graduate School, Chinese Academy of Sciences, Beijing, 100049, China

    Ming Cong, Duojiao Ni & Ling Li

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Correspondence to Linsheng Song.

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Cong, M., Ni, D., Song, L. et al. Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri . Mol Biol Rep 36, 1451–1459 (2009). https://doi.org/10.1007/s11033-008-9335-z

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