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Molecular cloning and characterisation of prophenoloxidase (ProPO) cDNA from Fenneropenaeus chinensis and its transcription injected by Vibrio anguillarum

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Abstract

The prophenoloxidase(ProPO) gene was cloned from haemocytes of Chinese shrimp Fenneropenaeus chinensis by Rapid Amplification Complementary DNA Ends (RACE) method. The full-length cDNA of prophenoloxidase gene consists of 3040 bp with a 2061 bp Open Reading Frame (ORF), encoding 686 amino acids. Phylogenetic analysis revealed that it belongs to insect-type invertebrate prophenoloxidase gene family. To understand ProPO reaction for pathogeny’s challenge in shrimp, the expressions of ProPO in different tissues were studied by real-time PCR after challenged by Vibrio anguillarum. The results showed that the expression level of ProPO gene in haemocytes was highest among three studied tissues including haemocytes, lymphoid organ and hepatopancreas. The time-course change of ProPO mRNA levels in challenge experiment showed that ProPO mRNA transcripts had the biggest change extent in lymphoid organ.

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Acknowledgements

This work was supported by grants from Major State Basic Research Development Program of China (973 program): 2006CB101804 and National High-Tech Research and Development Program of China (863 program) 2006AA09Z424.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China

    Hongwei Gao, Fuhua Li, Bo Dong, Qingli Zhang & Jianhai Xiang

  2. Shandong Entry-Exit Inspection and Quarantine Bureau of People’s Republic of China, Qingdao, China

    Hongwei Gao

  3. Graduate School of Chinese Academy of Sciences, Beijing, China

    Hongwei Gao

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  1. Hongwei Gao
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  2. Fuhua Li
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  3. Bo Dong
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  4. Qingli Zhang
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  5. Jianhai Xiang
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Correspondence to Jianhai Xiang.

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Gao, H., Li, F., Dong, B. et al. Molecular cloning and characterisation of prophenoloxidase (ProPO) cDNA from Fenneropenaeus chinensis and its transcription injected by Vibrio anguillarum . Mol Biol Rep 36, 1159–1166 (2009). https://doi.org/10.1007/s11033-008-9292-6

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  • DOI: https://doi.org/10.1007/s11033-008-9292-6

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