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Differential expression of ubiquitin-conjugating enzyme E2r in the developing ovary and testis of penaeid shrimp Marsupenaeus japonicus

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Abstract

In order to identify genes involved in oogenesis and spermatogenesis in penaeid shrimp Marsupenaeus japonicus, a modified annealing control primer (ACP) system was adapted to identify genes differentially expressed in ovary and testis at different developmental stages. By using 20 pairs of ACP primers, 8 differentially expressed genes were obtained. One of these genes is ubiquitin-conjugating enzyme E2r (UBE2r). Bioinformatics analyses show that this gene encodes a protein of 241 amino acids with a predicted molecular mass of 27.4 kDa. Real time PCR analyses demonstrated that the expression level changed significantly in the developing testis and ovary. In the stage 2 of testis, it reached its highest expression level, the lowest expression level present in the stage 1 of ovary. The significantly different expression levels in developing testis and ovary suggest that UBE2r has an important role in oogenesis and spermatogenesis. This article is the first report of UBE2r in crustaceans and also is the first report showing that UBE2r is differentially expressed at different stages of the developing ovary and testis in an animal.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 30070597, 30571430). We thank Mr. Scot Libants (Department of Fisheries & Wildlife, Michigan State University, East Lansing, Michigan, USA) and Mr. Ion Beldorth (Department of Chemistry & Biochemistry, Texas State University, San Marcos, Texas, USA) for critical reading of the manuscript.

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Authors and Affiliations

  1. The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China

    Bingling Shen, Yilei Wang, Guodong Wang, Yun Chen, Peng Lin, Shuhong Wang & Zhihua Zou

  2. Molecular Biosciences Research Group, Department of Chemistry & Biochemistry, Texas State University, San Marcos, TX, 78666, USA

    Ziping Zhang

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  1. Bingling Shen
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Correspondence to Ziping Zhang or Yilei Wang.

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Shen, B., Zhang, Z., Wang, Y. et al. Differential expression of ubiquitin-conjugating enzyme E2r in the developing ovary and testis of penaeid shrimp Marsupenaeus japonicus . Mol Biol Rep 36, 1149–1157 (2009). https://doi.org/10.1007/s11033-008-9291-7

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