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A novel SHV-type β-lactamase variant (SHV-89) in clinical isolates in China

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Abstract

Two clinical strains of Klebsiella pneumoniae (K. pneumoniae) and one isolate of Escherichia coli (E. coli) were collected from two large general hospitals in China. Conjugation experiment, susceptibility testing, isoelectric focusing, PCR, and sequencing techniques as well as clone, expression, purification and kinetics were carried out to describe the characterization of the novel SHV-tpye enzyme. The analysis of plasmid profiling and pulsed-field gel electrophoresis of the novel enzyme were performed to investigate epidemiology. These isolates had CTX-M-14 and SHV-89 β-lactamases. SHV-89 β-lactamase of pI 7.6 is a novel variant with two substitutions compared with the sequence of SHV-1: Leu35Gln and Met129Val. Its gene also had two silent mutations at positions 369 and 774, respectively. The results of substrate profiles and MIC determinations showed the activity of the novel enzyme was insufficient for the enzyme to count as an extended-spectrum β-lactamase (ESBL). The substrates of the enzyme were also characterized. Furthermore, the three novel SHV enzyme-producing strains were epidemiologically unrelated. The emergence of a novel SHV-type β-lactamase is rarely described in other areas. This study illustrates the importance of molecular survelliance in tracking SHV-producing strains in large teaching hospitals and emphasizes the need for epidemiological monitoring.

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Acknowledgement

This study is supported by National Natural Science Foundation of China (No.30571654).

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Authors and Affiliations

  1. Department of Infectious Diseases, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, China

    Jia-Bin Li, Jun Cheng, Qian Wang, Yan Chen & Ying Ye

  2. Clinical Medicine Post-doctorate Position of Anhui Medical University, Hefei, 230032, China

    Jia-Bin Li & Xue-Jun Zhang

  3. Institute of Dermatology, Anhui Medical University, Hefei, 230032, China

    Xue-Jun Zhang

Authors
  1. Jia-Bin Li
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  2. Jun Cheng
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  3. Qian Wang
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  4. Yan Chen
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  5. Ying Ye
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  6. Xue-Jun Zhang
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Corresponding authors

Correspondence to Jia-Bin Li or Xue-Jun Zhang.

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J.-B. Li and J. Cheng contributed equally to this work.

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Li, JB., Cheng, J., Wang, Q. et al. A novel SHV-type β-lactamase variant (SHV-89) in clinical isolates in China. Mol Biol Rep 36, 1141–1148 (2009). https://doi.org/10.1007/s11033-008-9290-8

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  • DOI: https://doi.org/10.1007/s11033-008-9290-8

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