Abstract
Lung cancer is one of the leading causes of death from cancer. Both immune cells and tumor cells play a key role in lung cancer immunity by secretion of cytokines and developing type-2 cell-mediated immune response. IL-13 is an immunoregulatory cytokine affecting tumor immunosurveillance by deviation of immune response from Th1 to Th2. In the present study we sought to determine the association of single nucleotide polymorphisms (SNPs) of IL-13 gene at positions +2044 (G/A) and −1055 (C/T) and lung cancer. One hundred forty one patients and 113 controls were recruited; control group was subdivided into smoker and nonsmoker individuals for serum detection. Genotyping was carried out by PCR-RFLP assay and IL-13 detection by ELISA method. No statistically significant difference was found in the frequency of genotypes, alleles, and haplotypes at positions +2044 (G/A) and −1055 (C/T) of IL-13 gene between lung cancer patients and controls. Serum level of IL-13 was not detectable in both groups. The results of this study reveal that although +2044 (G/A) and −1055 (C/T) SNPs in IL-13 are implicated in some pulmonary processes, they do not confer susceptibility to lung cancer in Iranian population.
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Acknowledgements
The authors are grateful to both patients and control subjects and also wish to acknowledge Dr. Nasrollah Erfani for his kind help in haplotyping the data. This work was financially supported by Shiraz Institute for Cancer Research (Grant No. ICR82-95) and by Shiraz University of Medical Sciences, Shiraz, Iran (Grant No. 83-2373).
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Sameni, S., Ghayumi, M.A., Mortazavi, G. et al. Lack of association between interleukin-13 gene polymorphisms (−1055 C/T and +2044 G/A) in Iranian patients with lung cancer. Mol Biol Rep 36, 1001–1005 (2009). https://doi.org/10.1007/s11033-008-9273-9
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DOI: https://doi.org/10.1007/s11033-008-9273-9