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Molecular cloning and expression analysis of a heat shock protein (Hsp90) gene from black tiger shrimp (Penaeus monodon)

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Abstract

The techniques of homology cloning and anchored PCR were used to clone the Hsp90 gene from black tiger shrimp. The full length cDNA of black tiger shrimp Hsp90 (btsHsp90) contained a 5′ untranslated region (UTR) of 72 bp, an ORF (open reading frame) of 2160 bp encoding a polypeptide of 720 amino acids with an estimated molecular mass of 83-kDa and a 3′ UTR of 288 bp. The sequence of the coding region showed 90 and 84% homology with that of the Chiromantes haematocheir and Homo sapiens, respectively. Conserved signature sequences of Hsp90 gene family were found in the btsHsp90 deduced amino acid sequence. The temporal expressions of Hsp90 gene were constitutively in the black tiger shrimp tissues including liver, ovary, muscle, brain stomach, and heart, and their levels were markedly enhanced after 30-min heat treatment at 37°C. In ovarian maturation stages, the expression of btsHsp90 was strongest in the second stage, weaker in the fourth and first stage.

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Acknowledgments

This study was supported by National nature foundation of China (No. 30571447), National “863” Project of China (No. 2003AA603120 ) and Agriculture Department Project of China(06-05-01B).

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Authors and Affiliations

  1. Biotechnology and Aquiculture Laboratory, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingangxi Road, Guangzhou, 510300, P.R. China

    Shigui Jiang, Lihua Qiu, Falin Zhou, Jianhua Huang, Yihui Guo & Keng Yang

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  1. Shigui Jiang
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  2. Lihua Qiu
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Correspondence to Shigui Jiang.

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Jiang, S., Qiu, L., Zhou, F. et al. Molecular cloning and expression analysis of a heat shock protein (Hsp90) gene from black tiger shrimp (Penaeus monodon). Mol Biol Rep 36, 127–134 (2009). https://doi.org/10.1007/s11033-007-9160-9

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  • DOI: https://doi.org/10.1007/s11033-007-9160-9

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