Skip to main content
SpringerLink
Log in

An easy method to discover cell membrane antigen with atomic force microscopy

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Atomic force microscopy (AFM) obtains a high resolution at nanometer. In the current study, we found that, before and after adding CD34, CD44 or CD29 antibodies, the AFM images of mouse adipose tissue derived mesenchymal stem cells (ADMSCs) had significant changes in both cell morphous (shape) and the average roughness of cell membrane. However, there was no significant difference in the cell shape and the average roughness of cell membrane, after adding CD45 or CD144 antibodies. Therefore, we could use the AFM to scan cell shape or to calculate the average roughness changes of cell membrane to analyze the existence of cell membrane antigen qualitatively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Reference

  1. Chen Y, Cai JY (2002) Diseased red blood cells studied by atomic force microscopy [J]. Int J Nanosc 1(5–6):683–688

    Article  CAS  Google Scholar 

  2. Grandbois M, Dettmarkn W, Benoit M et al (2000) Affinity imaging of red blood cells using an atomic force microscope [J]. J Histochem Cytochem 48(5):719–724

    PubMed  CAS  Google Scholar 

  3. Walch M, Ziegler U, Groscurth P (2000) Effect of streptolysin O on the microelasticity of human platelets analyzed by atomic force microscopy [J]. Ultramicroscopy 82:259–276

    Article  PubMed  CAS  Google Scholar 

  4. Müller DJ, Schaber FA, Büldt G (1995b) Imaging purple membrane in aqueous solution at sub-nanometer resolution by atomic force microscopy [J]. Biophys J 68:1681–1686

    Article  PubMed  Google Scholar 

  5. Yu YG, Xu RX, Jiang XD et al (2005) The study of 3-dimensional structures of IgG with atomic force microscopy. Chin J Traumatol 8(5):277–282

    PubMed  CAS  Google Scholar 

  6. Parbin BC et al (1998) Probing recognition process between an antibody and an antigen using atomic force microscopy. Physicochem Eng Asp 143:53–57

    Article  Google Scholar 

  7. Hinterdorfer P, Baumgartner W, Gruber HJ et al (1996) Detection and localization of individual antibody–antigen recognition events by atomic force microscopy [J]. Proc Natl Acad Sci USA 93:3477–3481

    Article  PubMed  CAS  Google Scholar 

  8. Ros R, Schwesinger F, Anselmetti D et al (1998). Antigen binding forces of individually addressed single-chain Fv antibody molecules [J]. Proc Natl Acad Sci USA 95:7402–7405

    Article  PubMed  CAS  Google Scholar 

  9. Willemsen OH, Snel MME, van der Werf KO et al (1998) Simultaneous height and adhesion imaging of antibody–antigen interactions by atomic force microscopy [J]. Biophys J 75:2220–2228

    PubMed  CAS  Google Scholar 

  10. Zheng B, Cao B, Li G (2006) Mouse adipose-derived stem cells undergo multilineage differentiation in vitro but primarily osteogenic and chondrogenic differentiation in vivo. Tissue Eng 12(7):1891–1901

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

First I want to show my appreciation to my advisor Professor Yang Ting Shu. He gave some necessary revises of the language of my article. Second I want to thank the coauthors of the article, because they help me to collect materials and organize the article.

Author information

Authors and Affiliations

  1. Laboratory of Cardiovascular Medical Department, The General Hospital of Chinese People of Liberation Army, Fu Xin Road 28, Beijing, 100853, China

    Wang Shu, Yang Ting Shu, Han Bao Shi, Liu Xiao Fei, Wang Lei & Qi Guan ming

Authors
  1. Wang Shu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yang Ting Shu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Han Bao Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Liu Xiao Fei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wang Lei
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qi Guan ming
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yang Ting Shu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shu, W., Shu, Y.T., Shi, H.B. et al. An easy method to discover cell membrane antigen with atomic force microscopy. Mol Biol Rep 35, 557–561 (2008). https://doi.org/10.1007/s11033-007-9122-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-007-9122-2

Keywords

Search

Navigation