Skip to main content
SpringerLink
Log in

Antioxidant action of Andrographis paniculata on lymphoma

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Regulation of the balance between production of reactive oxygen species (ROS) by cellular processes and its removal by antioxidant defense system maintains normal physiological processes. Any condition leading to increased ROS results in oxidative stress which has been related with a number of diseases including cancer. Improvement in antioxidant defense system is required to overcome the damaging effects of oxidative stress. Therefore in the present study, effect of the aqueous extract of a medicinal plant Andrographis paniculata (AP) on antioxidant defense system in liver is investigated in lymphoma bearing AKR mice. Estimating catalase, superoxide dismutase and glutathione S transferase monitored the antioxidant action. Oral administration of the aqueous extract of A. paniculata in different doses causes a significant elevation of catalase, superoxide dismutase and glutathione S transferase activities. It reveals the antioxidant action of the aqueous extract of AP, which may play a role in the anticarcinogenic activity by reducing the oxidative stress. LDH activity is known to increase in various cancers due to hypoxic condition. Lactate dehydrogenase is used as tumor marker. We find a significant decrease in LDH activity on treatment with AP, which indicates a decrease in carcinogenic activity. A comparison with Doxorubicin (DOX), an anticancerous drug, indicates that the aqueous extract of AP is more effective than DOX with respect to its effect on catalase, superoxide dismutase, glutathione S transferase as well as on lactate dehydrogenase activities in liver of lymphoma bearing mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Kotamraju S, Konorev EA, Joseph J, Kalyaraman B (2000) Doxorubicin induced apoptosis in endothelial cells and cardiomyoytes is ameliorated by nitron spin traps and ebselen. J Bio Chem 275:33585–33592

    Article  CAS  Google Scholar 

  2. Dahanukar SA, Kulkarani RA, Rege NN (2000) Pharmacology of medicinal plants and natural products. Indian J Pharmacol 32:S81–S118

    CAS  Google Scholar 

  3. Choudhary BR, Haque SJ, Poddar MK (1987) In vivo and in vitro effects of kalmegh (Andographis paniculata) extract and andrographolide on hepatic microsomal drug metabolizing enzymes. Planta Med 53:135–140

    Article  Google Scholar 

  4. Shukla B, Visen PKS, Patnaik GK, Dhawan BM (1992) Choleretic effect of andrographolide in rats and guinea pigs. Planta Med 58:146–149

    Article  PubMed  CAS  Google Scholar 

  5. Compos R, Garrido A, Guerra R, Valenzuella A (1987) Silybin dihemisuccinate protects against glutathione depletion and lipid peroxidation induced by acetaminophen on rat liver. Planta Med 53:135–140

    Article  Google Scholar 

  6. Chander R, Kapoor HK, Dhawan BN (1992) Effect of picroliv on glutathione metabolism in liver and brain of masstomys natalensis infected with Plasmodium berghei. Indian J Exp Biol 30:711–714

    PubMed  CAS  Google Scholar 

  7. Trivedi NP, Rawal UM (1998) Effect of aqueous extract of Andrograhis paniculata on liver tumor. Indian J Pharmacol 30:318–322

    Google Scholar 

  8. Pathak C, Vinayak M (2005) Modulation of Lactate dehydrogenase isozymes by modified base queuine. Mol Biol Rep 32:191–196

    Article  PubMed  CAS  Google Scholar 

  9. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  10. Woodbury W, Spencer AK, Stahman MA (1971) An improved procedure using ferricyanide for detecting catalase isozymes. Anal Biochem 44:301–305

    Article  PubMed  CAS  Google Scholar 

  11. Aebi H (1974) Catalase. In: Bergmeyer HU (ed) Method in enzymology, vol 2. Academic press, New York, pp 673–678

    Google Scholar 

  12. Winterbourn CC, Hawkins RE, Brian M, Carrell RW (1975) The estimation of red cell superoxide dismutase activity. J Lab Clin Med 85:337–341

    PubMed  CAS  Google Scholar 

  13. Beauchamp C, Fridovich I (1971) Superoxide dismutase: an improved assays and an assay applicable to acrylamide gel. Anal Biochem 44:276–287

    Article  PubMed  CAS  Google Scholar 

  14. Habig WH, Michael PJ, Jakoby WB (1974) Glutathione S transferase: the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139

    PubMed  CAS  Google Scholar 

  15. Hurd RE, Freeman DM (1989) Metabolic specific proton magnetic resonance imaging. PNAS 86:4402–4406

    Article  PubMed  CAS  Google Scholar 

  16. Schupp DG, Merkle H, Ellerman JM, Ke Y, Garwood M (1993) Localized detection of glioma glycolysis using edited 1H MRS. Magn Reson Med 30:18–27

    Article  PubMed  CAS  Google Scholar 

  17. He Q, Shungu DC, van Zijl PC, Bhujwalla ZM, Glickson JD (1995) Single scan in vivo lactate editing with complete lipid and water suppression by selective multiple-quantum-coherence transfer (Sel-MQC) with application to tumors. J Magn Reson B 106:203–211

    Article  PubMed  CAS  Google Scholar 

  18. Brizel DM, Schoeder T, Scher RL, Walenta S, Clough RW, Dewhirst MW, Mueller-Klieser W (2001) Elevated tumor lactate concentrations predict for an increased risk of metastasis in head and neck cancer. Int J Radiat Oncol Biol Phys 51:349–353

    PubMed  CAS  Google Scholar 

  19. Li SS, Fitch WM, Pan YC, Shaerif FS (1983) Evolutionary relationships of vertebrate lactate dehydrogenase isozymes A4 (muscle), B4 (heart) and C4 (testis). J Biol Chem 258:7029–7032

    PubMed  CAS  Google Scholar 

  20. Bachur NRS, Gordon SL, Gee MV (1977) Anthracycline antibiotic augmentation of microsomal electron transport and free radical formation. Mol Pharmacol 13:901–910

    PubMed  CAS  Google Scholar 

  21. Koul IB, Kapil A (1994) Effect of diterpenes from Andrographis paniculata on antioxidant defense system and lipid peroxidation. Indian J Pharmacol 26:296–300

    CAS  Google Scholar 

  22. Childs AC, Phaneuf SL, Dirks AJ, Philips T, Leeuwenburgh C (2002) Doxorubicin treatment in vivo causes cytochrome c release and cardiomyoyte apoptosis, as well as increased mitochondrial efficiency, superoxide dismutase activity, and Bcl-2: Bax ratio. Cancer Res 62:4592–4598

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

Nibha Verma thanks Indian Council of Medical Research, New Delhi, India for providing senior research fellowship.

Author information

Authors and Affiliations

  1. Biochemistry & Molecular Biology Lab, Department of Zoology, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India

    Nibha Verma & Manjula Vinayak

Authors
  1. Nibha Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manjula Vinayak
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Manjula Vinayak.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Verma, N., Vinayak, M. Antioxidant action of Andrographis paniculata on lymphoma. Mol Biol Rep 35, 535–540 (2008). https://doi.org/10.1007/s11033-007-9119-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-007-9119-x

Keywords

Search

Navigation