Abstract
To distinguish the cytoplasm of Danio rerio from that of Gobiocypris rarus, we cloned G. rarus COXI and constructed cytoplasmic molecular markers at the high identity domains of COXI by mutated primer PCR (MP-PCR for short). Then Sybr Green I was used to detect the single amplicon. As a result, we succeeded in getting the cytoplasmic molecular markers, G.M COXI and Z.M COXI, by MP-PCR strategy. They were used to detect the sperm-derived mtDNA in the sexual hybrid embryos (D. rerio ♀ × G. rarus ♂) before the sphere stage. In the present study, all results demonstrate that MP-PCR approach and Sybr Green I detection are feasible to construct the molecular markers to identify genes that shared high identity.
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Acknowledgements
This work was supported by the State Key Fundamental Research of China (Grant Nos. 2004CB117402 and 2004CB117406) and the National Natural Science Foundation of China (Grant No. 90208024). In addition, the authors are grateful to Ms. Ming Li for supplying experimental materials.
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Pei, D.S., Sun, Y.H. & Zhu, Z.Y. Construction of cytoplasmic molecular markers distinguishing Danio rerio from Gobiocypris rarus at high identity domains based on MP-PCR strategy and Sybr Green I detection. Mol Biol Rep 35, 45–50 (2008). https://doi.org/10.1007/s11033-006-9050-6
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DOI: https://doi.org/10.1007/s11033-006-9050-6