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Cloning and characterization of the human gene DERP6, which activates transcriptional activities of p53

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Abstract

We report here the cloning and characterization of the human gene DERP6, isolated from human testis cDNA library, and mapped to 17p13.1 by searching the UCSC genomic database. The DERP6 cDNA consists of 1486 nucleotides and has a 316-amino acids open reading frame. The Northern hybridization analysis showed that DERP6 was ubiquitously expressed in all the 16 adult tissues, especially highly expressed in heart, brain, liver, skeletal muscle and testis. DERP6 protein was located in cytoplasm when overexpressed in cultured cells. Reporter gene assays showed that overexpression of DERP6 in cells activated the transcriptional activities of p53. These results indicate that the human gene DERP6 may be involved in p53-mediated gene transcription.

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Abbreviations

BLAT:

BLAST-like Alignment Tool

DAPI:

4,6-diamidino-2-phenylindole

DERP6:

dermal papilla derived protein 6

Mdm2:

mouse double minute 2

MTN:

multiple tissue Northern blots

References

  1. AJ Levine (1997) Cell 88 323–331 Occurrence Handle9039259 Occurrence Handle1:CAS:528:DyaK2sXhtFahtLg%3D Occurrence Handle10.1016/S0092-8674(00)81871-1

    Article  PubMed  CAS  Google Scholar 

  2. S Bates KH Vousden (1999) Cell. Mol. Life. Sci. 55 28–37 Occurrence Handle10065149 Occurrence Handle1:CAS:528:DyaK1MXhtV2lsLw%3D Occurrence Handle10.1007/s000180050267

    Article  PubMed  CAS  Google Scholar 

  3. E Appella CW Anderson (2001) Eur. J. Biochem. 268 2764–2772 Occurrence Handle11358490 Occurrence Handle1:CAS:528:DC%2BD3MXktVSktr8%3D Occurrence Handle10.1046/j.1432-1327.2001.02225.x

    Article  PubMed  CAS  Google Scholar 

  4. M Ashcroft Y Taya KH Vousden (2000) Mol. Cell. Biol. 20 3224–3233 Occurrence Handle10757806 Occurrence Handle1:CAS:528:DC%2BD3cXivFSrs78%3D Occurrence Handle10.1128/MCB.20.9.3224-3233.2000

    Article  PubMed  CAS  Google Scholar 

  5. C Chao S Saito J Kang CW Anderson E Appella Y Xu (2000) EMBO J. 19 4967–4975 Occurrence Handle10990460 Occurrence Handle1:CAS:528:DC%2BD3cXntVartLw%3D Occurrence Handle10.1093/emboj/19.18.4967

    Article  PubMed  CAS  Google Scholar 

  6. KM Ryan AC Phillips KH Vousden (2001) Curr. Opin. Cell. Biol. 13 332–337 Occurrence Handle11343904 Occurrence Handle1:CAS:528:DC%2BD3MXktVaju7g%3D Occurrence Handle10.1016/S0955-0674(00)00216-7

    Article  PubMed  CAS  Google Scholar 

  7. J Momand HH Wu G Dasgupta (2000) Gene. 242 15–29 Occurrence Handle10721693 Occurrence Handle1:CAS:528:DC%2BD3cXhsFCjtrs%3D Occurrence Handle10.1016/S0378-1119(99)00487-4

    Article  PubMed  CAS  Google Scholar 

  8. MS Rodriguez JM Desterro S Lain DP Lane RT Hay (2000) Mol. Cell. Biol. 20 8458–8467 Occurrence Handle11046142 Occurrence Handle1:CAS:528:DC%2BD3cXotFWrtLw%3D Occurrence Handle10.1128/MCB.20.22.8458-8467.2000

    Article  PubMed  CAS  Google Scholar 

  9. C Blattner T Hay DW Meek DP Lane (2002) Mol. Cell. Biol. 22 6170–6182 Occurrence Handle12167711 Occurrence Handle1:CAS:528:DC%2BD38Xmt1ent7s%3D Occurrence Handle10.1128/MCB.22.17.6170-6182.2002

    Article  PubMed  CAS  Google Scholar 

  10. H Zheng H You XZ Zhou SA Murray T Uchida G Wulf L Gu X Tang KP Lu ZX Xiao (2002) Nature 419 849–853 Occurrence Handle12397361 Occurrence Handle1:CAS:528:DC%2BD38XovF2hs7s%3D Occurrence Handle10.1038/nature01116

    Article  PubMed  CAS  Google Scholar 

  11. P Zacchi M Gostissa T Uchida C Salvagno F Avolio S Volinia Z Ronai G Blandino C Schneider G Del Sal (2002) Nature 419 853–857 Occurrence Handle12397362 Occurrence Handle1:CAS:528:DC%2BD38XovF2hsL4%3D Occurrence Handle10.1038/nature01120

    Article  PubMed  CAS  Google Scholar 

  12. GM Wulf YC Liou A Ryo SW Lee KP Lu (2002) J. Biol. Chem. 277 47976–47979 Occurrence Handle12388558 Occurrence Handle1:CAS:528:DC%2BD38Xptlehur0%3D Occurrence Handle10.1074/jbc.C200538200

    Article  PubMed  CAS  Google Scholar 

  13. Y Wu L Yu G Bi K Luo G Zhou S Zhao (2003) Gene 310 193–201 Occurrence Handle12801647 Occurrence Handle1:CAS:528:DC%2BD3sXktlOlt7k%3D Occurrence Handle10.1016/S0378-1119(03)00551-1

    Article  PubMed  CAS  Google Scholar 

  14. J Sambrook EF Fritch T Maniatis (1989) Molecular Cloning: A Laboratory Manual EditionNumber2 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY

    Google Scholar 

  15. GS Jimenez SH Khan JM Stommel GM Wahl (1999) Oncogene 18 7656–7665 Occurrence Handle10618705 Occurrence Handle1:CAS:528:DC%2BD3cXmt1SrsA%3D%3D Occurrence Handle10.1038/sj.onc.1203013

    Article  PubMed  CAS  Google Scholar 

  16. NH Chehab A Malikzay M Appel TD Halazonetis (2000) Genes. Dev. 14 278–288 Occurrence Handle10673500 Occurrence Handle1:CAS:528:DC%2BD3cXhsVKhsr0%3D

    PubMed  CAS  Google Scholar 

  17. SY Shieh J Ahn K Tamai Y Taya C Prives (2000) Genes. Dev. 14 289–300 Occurrence Handle10673501 Occurrence Handle1:CAS:528:DC%2BD3cXhsVKhsro%3D

    PubMed  CAS  Google Scholar 

  18. A Hirao YY Kong S Matsuoka A Wakeham J Ruland H Yoshida D Liu SJ Elledge TW Mak (2000) Science 287 1824–1827 Occurrence Handle10710310 Occurrence Handle1:CAS:528:DC%2BD3cXhvVWktrg%3D Occurrence Handle10.1126/science.287.5459.1824

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai, 200433, P.R. China

    Jian Yuan, Wenwen Tang, Kuntian Luo, Xinya Chen, Xiuting Gu, Bo Wan & Long Yu

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  1. Jian Yuan
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  2. Wenwen Tang
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  3. Kuntian Luo
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  4. Xinya Chen
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  5. Xiuting Gu
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  6. Bo Wan
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  7. Long Yu
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Correspondence to Long Yu.

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Yuan, J., Tang, W., Luo, K. et al. Cloning and characterization of the human gene DERP6, which activates transcriptional activities of p53. Mol Biol Rep 33, 151–158 (2006). https://doi.org/10.1007/s11033-006-6273-5

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  • DOI: https://doi.org/10.1007/s11033-006-6273-5

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