Skip to main content
SpringerLink
Log in

Regulation of Osteoblast Gene Expression and Phenotype by Polylactide-fatty Acid Surfaces

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Cell function is influenced by surface structure and molecules. Molecules that enhance cellular differentiation can be applied to tissue scaffold surfaces to stimulate endogenous tissue regeneration. The application of this approach to bone implants yields surfaces coated with factors (proteins, peptides, etc...) that promote the differentiation of osteoblasts, the cells that make bone. Increased bone formation leads to increased healing and union of the implant with endogenous bone. To obtain better control over surface coating we developed PLLA copolymers with allyl (PLLA-co-DAG) and 3-hydroxypropyl (PLLA-co-HP) side chains to which we can attach functional groups. Given the potential of fatty acids being able to incorporate into lipid bilayers and/or influence gene expression, we grafted different fatty acid side chains to PLLA-co-HP by esterifying the corresponding fatty acids with the PLLA-co-HP 3-hydroxypropyl side chains. The effects of the polymer modifications on osteoblasts were then evaluated. While cellular morphology differed between surface coatings, they did not reflect changes in cellular phenotype. Changes in gene expression were most evident with arachidonate and 3-hydroxypropyl side-chains which exhibited osteoblast differentiating capabilities. Linoleate, myristate, oleate, and stearate ester side-chains did not have a significant influence on osteoblast phenotype. Growth characteristics of osteoblasts did not differ between the fatty acid copolymer films, although cells grown on PLLA-co-HP exhibited a trend toward increased growth. Taken together our findings demonstrate that surface fatty acid composition can impact osteoblast phenotype.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

TGF-beta:

transforming growth factor-beta

BMPs:

bone morphogenic proteins

IGF-1:

insulin like growth factor-1

HGF:

hepatocyte growth factor

BrdU:

bromodeoxyuridine

PUFA:

polyunsaturated fatty acids

DAG:

(3,5-diallyl)glycolide

PLA:

poly-lactic acid

PLLA:

poly-l-lactic acid

PLLA-co-DAG:

poly[l-lactide-co-(3,5-diallyl)glycolide]

PLLA-co-HP:

poly[l-lactide-co-(3,5-(3-hydroxypropyl)glycolide]

PLLA-g-D4Ach:

poly[l-lactide-g-arachidonate]

PLLA-g-Ste:

poly[l-lactide-g-stearate]

PLLA-g-Myr:

poly[l-lactide-g-myristate]

PLLA-g-Ole:

poly [l-lactide-g-oleate]

PLLA-g-Lin:

poly[l-lactide-g-linoleate]

M w :

weight-average molecular weight

M n :

number-average molecular weight

PDI:

polydispersity indices

BBA:

4-tert-butylbenzylalcohol

9-BBN:

9-borabicyclo[3.3.1]nonane

DMAP:

4-dimethylamino(pyridine)

DCC:

Dicyclohexylcarbodiimide

THF:

tetrahydrofuran

References

  1. K Kieswetter Z Schwartz TW Hummert DL Cochran J Simpson DD Dean BD Boyan (1996) J. Biomed. Mater. Res. 32 55–63 Occurrence Handle10.1002/(SICI)1097-4636(199609)32:1<55::AID-JBM7>3.0.CO;2-O Occurrence Handle8864873 Occurrence Handle1:CAS:528:DyaK28XltFGksbY%3D

    Article  PubMed  CAS  Google Scholar 

  2. K Kieswetter Z Schwartz DD Dean BD Boyan (1996) Crit. Rev. Oral Biol. Med. 7 329–345 Occurrence Handle8986395 Occurrence Handle1:STN:280:ByiC3sbnvFM%3D

    PubMed  CAS  Google Scholar 

  3. JY Martin Z Schwartz TW Hummert DM Schraub J Simpson J Lankford SuffixJr. DD Dean DL Cochran BD Boyan (1995) J. Biomed. Mater. Res. 29 389–401 Occurrence Handle7542245 Occurrence Handle1:CAS:528:DyaK2MXktFWmu7Y%3D

    PubMed  CAS  Google Scholar 

  4. J Lincks BD Boyan CR Blanchard CH Lohmann Y Liu DL Cochran DD Dean Z Schwartz (1998) Biomaterials 19 2219–2232 Occurrence Handle10.1016/S0142-9612(98)00144-6 Occurrence Handle9884063 Occurrence Handle1:CAS:528:DyaK1MXkt1Gn

    Article  PubMed  CAS  Google Scholar 

  5. DM Brunette (1988) Int. J. Oral Maxillofac. Implants 3 231–246 Occurrence Handle3075965 Occurrence Handle1:STN:280:BiaA38nlsVE%3D

    PubMed  CAS  Google Scholar 

  6. DW Murray T Rae N Rushton (1989) J. Bone Joint Surg. Br. 71 632–637 Occurrence Handle2670951 Occurrence Handle1:STN:280:BiaA2Mbks1U%3D

    PubMed  CAS  Google Scholar 

  7. BD Boyan TW Hummert DD Dean Z Schwartz (1996) Biomaterials 17 137–146 Occurrence Handle10.1016/0142-9612(96)85758-9 Occurrence Handle8624390 Occurrence Handle1:CAS:528:DyaK28Xnslersg%3D%3D

    Article  PubMed  CAS  Google Scholar 

  8. Z Schwartz BD Boyan (1994) J. Cell. Biochem. 56 340–347 Occurrence Handle10.1002/jcb.240560310 Occurrence Handle7876327 Occurrence Handle1:CAS:528:DyaK2MXitFygsr4%3D

    Article  PubMed  CAS  Google Scholar 

  9. YY Hu C Zhang R Lu JQ Xu D Li (2003) Chin. J. Traumatol. 6 67–74 Occurrence Handle12659700 Occurrence Handle1:CAS:528:DC%2BD3sXltlGktLc%3D

    PubMed  CAS  Google Scholar 

  10. G Herr CH Hartwig C Boll W Kusswetter (1996) Acta Orthop. Scand. 67 606–610 Occurrence Handle9065076 Occurrence Handle1:STN:280:ByiC1cfmsFY%3D

    PubMed  CAS  Google Scholar 

  11. P Laffargue P Fialdes P Frayssinet M Rtaimate HF Hildebrand X Marchandise (2000) J. Biomed. Mater. Res. 49 415–421 Occurrence Handle10.1002/(SICI)1097-4636(20000305)49:3<415::AID-JBM15>3.0.CO;2-Z Occurrence Handle10602075 Occurrence Handle1:CAS:528:DC%2BD3cXltlGk

    Article  PubMed  CAS  Google Scholar 

  12. G Zambonin M Grano G Greco RO Oreffo JT Triffit (1999) Acta Orthop. Scand. 70 217–220 Occurrence Handle10366928 Occurrence Handle1:STN:280:DyaK1M3psFSjtg%3D%3D

    PubMed  CAS  Google Scholar 

  13. M Hossain R Irwin MJ Baumann LR McCabe (2005) Biomaterials 26 2595–2602 Occurrence Handle10.1016/j.biomaterials.2004.07.051 Occurrence Handle15585262 Occurrence Handle1:CAS:528:DC%2BD2cXhtVChsrzL

    Article  PubMed  CAS  Google Scholar 

  14. G Zambonin C Camerino G Greco V Patella B Moretti M Grano (2000) J. Bone Joint Surg. Br. 82 457–460 Occurrence Handle10.1302/0301-620X.82B3.9310 Occurrence Handle10813189 Occurrence Handle1:STN:280:DC%2BD3c3ntFaqtQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  15. MD Pierschbacher E Ruoslahti (1984) Nature 309 30–33 Occurrence Handle10.1038/309030a0 Occurrence Handle6325925 Occurrence Handle1:CAS:528:DyaL2cXksFSgsrY%3D

    Article  PubMed  CAS  Google Scholar 

  16. KC Dee TT Andersen R Bizios (1998) J. Biomed. Mater. Res. 40 371–377 Occurrence Handle10.1002/(SICI)1097-4636(19980605)40:3<371::AID-JBM5>3.0.CO;2-C Occurrence Handle9570067 Occurrence Handle1:CAS:528:DyaK1cXisVeqs70%3D

    Article  PubMed  CAS  Google Scholar 

  17. M Gilbert CM Giachelli PS Stayton (2003) J. Biomed. Mater. Res. 67A 69–77 Occurrence Handle10.1002/jbm.a.10053 Occurrence Handle1:CAS:528:DC%2BD3sXotF2msbo%3D

    Article  CAS  Google Scholar 

  18. M Gilbert WJ Shaw JR Long K Nelson GP Drobny CM Giachelli PS Stayton (2000) J. Biol. Chem. 275 16213–16218 Occurrence Handle10748043 Occurrence Handle1:CAS:528:DC%2BD3cXjvFaitLo%3D

    PubMed  CAS  Google Scholar 

  19. A Rezania CH Thomas AB Branger CM Waters KE Healy (1997) J. Biomed. Mater. Res. 37 9–19 Occurrence Handle10.1002/(SICI)1097-4636(199710)37:1<9::AID-JBM2>3.0.CO;2-W Occurrence Handle9335344 Occurrence Handle1:CAS:528:DyaK2sXmsFWgsb4%3D

    Article  PubMed  CAS  Google Scholar 

  20. DA Puleo A Nanci (1999) Biomaterials 20 2311–2321 Occurrence Handle10.1016/S0142-9612(99)00160-X Occurrence Handle10614937 Occurrence Handle1:CAS:528:DC%2BD3cXlsVWm

    Article  PubMed  CAS  Google Scholar 

  21. JP Gorski (1992) Calcif. Tissue Int. 50 391–396 Occurrence Handle10.1007/BF00296767 Occurrence Handle1596774 Occurrence Handle1:CAS:528:DyaK38XkvVOhtbw%3D

    Article  PubMed  CAS  Google Scholar 

  22. M Johnsson MJ Levine GH Nancollas (1993) Crit. Rev. Oral Biol. Med. 4 371–378 Occurrence Handle8396998 Occurrence Handle1:STN:280:ByyA1crjvFA%3D

    PubMed  CAS  Google Scholar 

  23. AL Boskey (1995) Ann. NY Acad. Sci. 760 249–256 Occurrence Handle7785899 Occurrence Handle1:CAS:528:DyaK2MXovVSitrY%3D

    PubMed  CAS  Google Scholar 

  24. A Nanci JD Wuest L Peru P Brunet V Sharma S Zalzal MD McKee (1998) J. Biomed. Mater. Res. 40 324–335 Occurrence Handle10.1002/(SICI)1097-4636(199805)40:2<324::AID-JBM18>3.0.CO;2-L Occurrence Handle9549628 Occurrence Handle1:CAS:528:DyaK1cXhvVersrg%3D

    Article  PubMed  CAS  Google Scholar 

  25. DA Puleo (1995) J. Biomed. Mater. Res. 29 951–957 Occurrence Handle10.1002/jbm.820290806 Occurrence Handle7593038 Occurrence Handle1:CAS:528:DyaK2MXnt1CrsLo%3D

    Article  PubMed  CAS  Google Scholar 

  26. DA Puleo (1997) J. Biomed. Mater. Res. 37 222–228 Occurrence Handle10.1002/(SICI)1097-4636(199711)37:2<222::AID-JBM11>3.0.CO;2-G Occurrence Handle9358315 Occurrence Handle1:CAS:528:DyaK2sXntValsLg%3D

    Article  PubMed  CAS  Google Scholar 

  27. M Mrksich CS Chen Y Xia LE Dike DE Ingber GM Whitesides (1996) Proc. Natl. Acad. Sci. USA 93 10775–10778 Occurrence Handle10.1073/pnas.93.20.10775 Occurrence Handle8855256 Occurrence Handle1:CAS:528:DyaK28XmtVOltb8%3D

    Article  PubMed  CAS  Google Scholar 

  28. M Tanahashi T Matsuda (1997) J. Biomed. Mater. Res. 34 305–315 Occurrence Handle10.1002/(SICI)1097-4636(19970305)34:3<305::AID-JBM5>3.0.CO;2-O Occurrence Handle9086400 Occurrence Handle1:CAS:528:DyaK2sXhsFGksLg%3D

    Article  PubMed  CAS  Google Scholar 

  29. Y Nakanishi G Chen H Komuro T Ushida S Kaneko T Tateishi M Kaneko (2003) J. Pediatr. Surg. 38 1781–1784 Occurrence Handle10.1016/j.jpedsurg.2003.08.034 Occurrence Handle14666467

    Article  PubMed  Google Scholar 

  30. SH Choi TG Park (2002) J. Biomater. Sci. Polym. Ed. 13 1163–1173 Occurrence Handle12484491 Occurrence Handle1:CAS:528:DC%2BD38XpsFCktrY%3D

    PubMed  CAS  Google Scholar 

  31. J Mayer E Karamuk T Akaike E Wintermantel (2000) J. Control Release 64 81–90 Occurrence Handle10.1016/S0168-3659(99)00136-4 Occurrence Handle10640647 Occurrence Handle1:CAS:528:DC%2BD3cXks1Gltg%3D%3D

    Article  PubMed  CAS  Google Scholar 

  32. F Kandziora H Bail G Schmidmaier G Schollmeier M Scholz C Knispel T Hiller R Pflugmacher T Mittlmeier M Raschke NP Haas (2002) J. Neurosurg. 97 40–48 Occurrence Handle12120650 Occurrence Handle1:CAS:528:DC%2BD38XlvVKgu7o%3D

    PubMed  CAS  Google Scholar 

  33. B Wildemann F Kandziora G Krummrey N Palasdies NP Haas M Raschke G Schmidmaier (2004) J. Control Release 95 249–256 Occurrence Handle10.1016/j.jconrel.2003.11.014 Occurrence Handle14980773 Occurrence Handle1:CAS:528:DC%2BD2cXhsVCmsrs%3D

    Article  PubMed  CAS  Google Scholar 

  34. MA Aronow LC Gerstenfeld TA Owen MS Tassinari GS Stein JB Lian (1990) J. Cell. Physiol. 143 213–221 Occurrence Handle10.1002/jcp.1041430203 Occurrence Handle2332447 Occurrence Handle1:CAS:528:DyaK3cXktVeltbY%3D

    Article  PubMed  CAS  Google Scholar 

  35. D Wang K Christensen K Chawla G Xiao PH Krebsbach RT Franceschi (1999) J. Bone Miner. Res. 14 893–903 Occurrence Handle10352097 Occurrence Handle1:CAS:528:DyaK1MXjvFKqtb0%3D

    PubMed  CAS  Google Scholar 

  36. LD Quarles DA Yohay LW Lever R Caton RJ Wenstrup (1992) J. Bone Miner. Res. 7 683–692 Occurrence Handle1414487 Occurrence Handle1:STN:280:ByyD3svktlU%3D

    PubMed  CAS  Google Scholar 

  37. C Ontiveros LR McCabe (2003) J. Cell. Biochem. 88 427–437 Occurrence Handle10.1002/jcb.10410 Occurrence Handle12532319 Occurrence Handle1:CAS:528:DC%2BD3sXhtFKjsbY%3D

    Article  PubMed  CAS  Google Scholar 

  38. T Komori H Yagi S Nomura A Yamaguchi K Sasaki K Deguchi Y Shimizu RT Bronson YH Gao M Inada M Sato R Okamoto Y Kitamura S Yoshiki T Kishimoto (1997) Cell 89 755–764 Occurrence Handle10.1016/S0092-8674(00)80258-5 Occurrence Handle9182763 Occurrence Handle1:CAS:528:DyaK2sXjs1ejtr4%3D

    Article  PubMed  CAS  Google Scholar 

  39. P Ducy R Zhang V Geoffroy AL Ridall G Karsenty (1997) Cell 89 747–754 Occurrence Handle10.1016/S0092-8674(00)80257-3 Occurrence Handle9182762 Occurrence Handle1:CAS:528:DyaK2sXjs1ejsbg%3D

    Article  PubMed  CAS  Google Scholar 

  40. JB Lian GS Stein JL Stein AJ Wijnen Particlevan (1998) J. Cell. Biochem. Suppl 30–31 62–72

    Google Scholar 

  41. S Mundlos F Otto C Mundlos JB Mulliken AS Aylsworth S Albright D Lindhout WG Cole W Henn JH Knoll MJ Owen R Mertelsmann BU Zabel BR Olsen (1997) Cell 89 773–779 Occurrence Handle10.1016/S0092-8674(00)80260-3 Occurrence Handle9182765 Occurrence Handle1:CAS:528:DyaK2sXjs1ejt74%3D

    Article  PubMed  CAS  Google Scholar 

  42. C Eriksson H Nygren K Ohlson (2004) Biomaterials 25 4759–4766 Occurrence Handle10.1016/j.biomaterials.2003.12.006 Occurrence Handle15120522 Occurrence Handle1:CAS:528:DC%2BD2cXjsFOnsLg%3D

    Article  PubMed  CAS  Google Scholar 

  43. EJ Chang HH Kim JE Huh IA Kim J Seung Ko CP Chung HM Kim (2005) Exp. Cell. Res. 303 197–206 Occurrence Handle15572039 Occurrence Handle1:CAS:528:DC%2BD2cXhtVaqt77K

    PubMed  CAS  Google Scholar 

  44. EJ Jansen RE Sladek H Bahar A Yaffe MJ Gijbels R Kuijer SK Bulstra NA Guldemond I Binderman LH Koole (2005) Biomaterials 26 4423–4431 Occurrence Handle10.1016/j.biomaterials.2004.11.011 Occurrence Handle15701371 Occurrence Handle1:CAS:528:DC%2BD2MXhtVejsrc%3D

    Article  PubMed  CAS  Google Scholar 

  45. K Geckeler R Wacker F Martini A Hack W Aicher (2003) Cell. Physiol. Biochem. 13 155–164 Occurrence Handle10.1159/000071866 Occurrence Handle12876386 Occurrence Handle1:CAS:528:DC%2BD3sXlvVajs7w%3D

    Article  PubMed  CAS  Google Scholar 

  46. BA Watkins Y Li HE Lippman S Feng (2003) Prostag. Leukot. Essent. Fatty Acids 68 387–398 Occurrence Handle1:CAS:528:DC%2BD3sXksVGksbs%3D

    CAS  Google Scholar 

  47. BA Watkins Y Li MF Seifert (2001) J. Am. Coll. Nutr. 20 410S–416S Occurrence Handle11603651 Occurrence Handle1:CAS:528:DC%2BD3MXnvVCju7c%3D

    PubMed  CAS  Google Scholar 

  48. G Priante L Bordin E Musacchio G Clari B Baggio (2002) Clin. Sci. (Lond) 102 403–409 Occurrence Handle1:CAS:528:DC%2BD38XjsFahsrY%3D

    CAS  Google Scholar 

  49. BA Watkins MF Seifert (2000) J. Am. Coll. Nutr. 19 478S–486S Occurrence Handle10963468 Occurrence Handle1:CAS:528:DC%2BD3cXmsFOht7c%3D

    PubMed  CAS  Google Scholar 

  50. DD Diascro SuffixJr. RL Vogel TE Johnson KM Witherup SM Pitzenberger SJ Rutledge DJ Prescott GA Rodan A Schmidt (1998) J. Bone Miner. Res. 13 96–106 Occurrence Handle9443795 Occurrence Handle1:CAS:528:DyaK1cXltVyqtQ%3D%3D

    PubMed  CAS  Google Scholar 

  51. Radino CP, Wang M, Baker G & Smith M (Manuscript in preparation)

  52. H Sudo HA Kodama Y Amagai S Yamamoto S Kasai (1983) J. Cell Biol. 96 191–198 Occurrence Handle10.1083/jcb.96.1.191 Occurrence Handle6826647 Occurrence Handle1:CAS:528:DyaL3sXmvFKmtA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  53. J Xie MJ Baumann LR McCabe (2004) J. Biomed. Mater. Res. 71A 108–117 Occurrence Handle10.1002/jbm.a.30140 Occurrence Handle1:CAS:528:DC%2BD2cXnvVWltbY%3D

    Article  CAS  Google Scholar 

  54. R Shu R McMullen MJ Baumann LR McCabe (2003) J. Biomed. Mater. Res. 67A 1196–1204 Occurrence Handle10.1002/jbm.a.20021 Occurrence Handle1:CAS:528:DC%2BD3sXpslGls7c%3D

    Article  CAS  Google Scholar 

  55. C Ontiveros R Irwin R Wiseman L McCabe (2004) J. Cell. Physiol. 200 169–174 Occurrence Handle10.1002/jcp.20054 Occurrence Handle15174088 Occurrence Handle1:CAS:528:DC%2BD2cXlslaqtbc%3D

    Article  PubMed  CAS  Google Scholar 

  56. S Botolin MC Faugere H Malluche M Orth R Meyer LR McCabe (2005) Endocrinology 146 3622–3631 Occurrence Handle10.1210/en.2004-1677 Occurrence Handle15905321 Occurrence Handle1:CAS:528:DC%2BD2MXntVahsr8%3D

    Article  PubMed  CAS  Google Scholar 

  57. ML Lewis M Hughes-Fulford (2000) J. Cell. Biochem. 77 127–134 Occurrence Handle10.1002/(SICI)1097-4644(20000401)77:1<127::AID-JCB13>3.0.CO;2-0 Occurrence Handle10679823 Occurrence Handle1:CAS:528:DC%2BD3cXhslygtbo%3D

    Article  PubMed  CAS  Google Scholar 

  58. M Yin GL Baker (1999) Macromolecules 32 7711–7718 Occurrence Handle10.1021/ma9907183 Occurrence Handle1:CAS:528:DyaK1MXms1ekurg%3D

    Article  CAS  Google Scholar 

  59. G Karsenty (2001) Endocrinology 142 2731–2733 Occurrence Handle10.1210/en.142.7.2731 Occurrence Handle11415989 Occurrence Handle1:CAS:528:DC%2BD3MXkslelsrk%3D

    Article  PubMed  CAS  Google Scholar 

  60. P Ducy (2000) Dev. Dyn. 219 461–471 Occurrence Handle10.1002/1097-0177(2000)9999:9999<::AID-DVDY1074>3.0.CO;2-C Occurrence Handle11084646 Occurrence Handle1:CAS:528:DC%2BD3cXovVSgsbY%3D

    Article  PubMed  CAS  Google Scholar 

  61. F Otto AP Thornell T Crompton A Denzel KC Gilmour IR Rosewell GW Stamp RS Beddington S Mundlos BR Olsen PB Selby MJ Owen (1997) Cell 89 765–771 Occurrence Handle10.1016/S0092-8674(00)80259-7 Occurrence Handle9182764 Occurrence Handle1:CAS:528:DyaK2sXjs1ejtrk%3D

    Article  PubMed  CAS  Google Scholar 

  62. B Lee K Thirunavukkarasu L Zhou L Pastore A Baldini J Hecht V Geoffroy P Ducy G Karsenty (1997) Nat. Genet. 16 307–310 Occurrence Handle10.1038/ng0797-307 Occurrence Handle9207800 Occurrence Handle1:CAS:528:DyaK2sXktFKjsL0%3D

    Article  PubMed  CAS  Google Scholar 

  63. AC Maurin PM Chavassieux E Vericel PJ Meunier (2002) Bone 31 260–266 Occurrence Handle10.1016/S8756-3282(02)00805-0 Occurrence Handle12110443 Occurrence Handle1:CAS:528:DC%2BD38XltFWgs7Y%3D

    Article  PubMed  CAS  Google Scholar 

  64. MT Conconi M Tommasini S Baiguera P Coppi ParticleDe PP Parnigotto GG Nussdorfer (2002) Int. J. Mol. Med. 10 451–456 Occurrence Handle12239592 Occurrence Handle1:CAS:528:DC%2BD38XotVCqsL4%3D

    PubMed  CAS  Google Scholar 

  65. HY Yin CM Havrilla JD Morrow NA Porter (2002) J. Am. Chem. Soc. 124 7745–7754 Occurrence Handle12083928 Occurrence Handle1:CAS:528:DC%2BD38XktlCgs7c%3D

    PubMed  CAS  Google Scholar 

  66. VA Roginskii IV Utkin (1991) Kinet. Catal. 32 732–737

    Google Scholar 

  67. Y Wan J Yang J Bei S Wang (2003) Biomaterials 24 3757–3764 Occurrence Handle12818548 Occurrence Handle1:CAS:528:DC%2BD3sXksFKks7g%3D

    PubMed  CAS  Google Scholar 

  68. G Khang J Choee J Rhee H Lee (2002) J. Appl. Polym. Sci. 85 1253–1262 Occurrence Handle10.1002/app.10680 Occurrence Handle1:CAS:528:DC%2BD38Xks12guro%3D

    Article  CAS  Google Scholar 

  69. Y Ikarashi T Tsuchiya M Kaniwa A Nakamura (2000) Biol. Pharm. Bull. 23 1470–1476 Occurrence Handle11145180 Occurrence Handle1:CAS:528:DC%2BD3cXos1ehsro%3D

    PubMed  CAS  Google Scholar 

  70. EJ Helmreich (2003) Biophys. Chem. 100 519–534 Occurrence Handle10.1016/S0301-4622(02)00303-4 Occurrence Handle12646388 Occurrence Handle1:CAS:528:DC%2BD3sXitFamu7c%3D

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Physiology and Radiology, Molecular Imaging Research Center, Michigan State University, 2201 Biomedical Physical Science Building, East Lansing, MI, 48824

    K Porter, M Hossain & L R McCabe

  2. Department of Chemistry, Michigan State University, 320 Chemistry Building, East Lansing, MI, 48824

    M Wang, C P Radano, G L Baker & M R Smith III

Authors
  1. K Porter
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M Hossain
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C P Radano
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G L Baker
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M R Smith III
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. L R McCabe
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L R McCabe.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Porter, K., Hossain, M., Wang, M. et al. Regulation of Osteoblast Gene Expression and Phenotype by Polylactide-fatty Acid Surfaces. Mol Biol Rep 33, 1–12 (2006). https://doi.org/10.1007/s11033-005-4535-2

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-005-4535-2

Keywords

Search

Navigation