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Identification and Characterization of RHEBL1, a Novel Member of Ras Family, which Activates Transcriptional Activities of NF-Kappa B

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Abstract

The Ras family of small GTPases regulates a wide variety of cellular functions that include cell growth, differentiation, and transformation. In this study, we identified and characterized a novel member of Ras family named RHEBL1, belonging to the Rheb branch of small GTPase proteins. The cDNA sequence contains an open reading frame of 551 bp, encoding a putative protein of 183 amino acid residues. The expression pattern of RHEBL1 showed that it was ubiquitously expressed in 17 tissues. RHEBL1 gene encodes a 20.69 kDa protein, localized in cytoplasm when overexpressed in COS7 cells. Reporter gene assays showed that overexpression of RHEBL1 in HEK 293T cells strongly activated the transcriptional activities of NF-kappa B, while the mutant (D60K) only weakly activates NF-kappa B-mediated transcription. Our findings suggest that RHEBL1 is a positive regulator of NF-kappa B-mediated gene transcription.

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Abbreviations

GAP:

GTPase-activating proteins

mTOR:

mammalian target of rapamycin

RHEB :

Ras Homologue Enrich in Brain

RHEBL1 :

Ras Homologue Enrich in Brain Like 1

TSC:

tuberous sclerosis complex

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai, 200433, P.R. China

    Jian Yuan, Yuxi Shan, Xinya Chen, Wenwen Tang, Kuntian Luo, Jun Ni, Bo Wan & Long Yu

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  1. Jian Yuan
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  2. Yuxi Shan
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  3. Xinya Chen
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  4. Wenwen Tang
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  6. Jun Ni
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  8. Long Yu
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Correspondence to Long Yu.

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These authors contributed equally to the work.

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Yuan, J., Shan, Y., Chen, X. et al. Identification and Characterization of RHEBL1, a Novel Member of Ras Family, which Activates Transcriptional Activities of NF-Kappa B. Mol Biol Rep 32, 205–214 (2005). https://doi.org/10.1007/s11033-005-0984-x

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