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C/EBPα and C/EBPβ Regulate Haptoglobin Gene Expression during Rat Liver Development and the Acute-phase Response

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Abstract

The participation of C/EBPα and C/EBPβ in the transcriptional regulation of the haptoglobin (Hp) gene throughout liver development and the acute-phase (AP) response was examined. Western immunoblot analysis revealed that the relative concentrations of C/EBPα and C/EBPβ increased during differentiation in two nuclear protein fractions – the nuclear extract and nuclear matrix. The AP reaction was accompanied by a decrease of the relative concentration of C/EBPα and an increase of C/EBPβ during development in both protein fractions. Using Western analysis after DNA-affinity chromatography it was observed that a 45 kDa C/EBPα isoform displayed a binding affinity towards the Hp gene hormone responsive element (HRE) in both pre- and postnatal livers. In the course of the AP response DNA binding of the 45 kDa isoform was detected only in the adult, when its binding affinity decreased. The 35 kDa C/EBPβ isoform exhibited a binding affinity towards the Hp HRE after the second week from birth, whereas the AP response promoted an enhanced binding of 35 kDa isoform after the first postnatal week. These results indicate that Hp gene transcription is regulated by C/EBPα during normal liver development, whereas C/EBPβ is involved in the AP regulation during the later phase of differentiation and in the adult.

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Authors and Affiliations

  1. Department of Molecular Biology, Institute for Biological Research, Despot Stefan Blvd 142, Belgrade, 11060, Serbia and Montenegro

    Svetlana Dinić, Desanka Bogojević, Miodrag Petrović, Goran Poznanović, Svetlana Ivanovič-Matić & Mirjana Mihailović

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  1. Svetlana Dinić
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  2. Desanka Bogojević
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  3. Miodrag Petrović
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  4. Goran Poznanović
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  5. Svetlana Ivanovič-Matić
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  6. Mirjana Mihailović
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Correspondence to Mirjana Mihailović.

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Dinić, S., Bogojević, D., Petrović, M. et al. C/EBPα and C/EBPβ Regulate Haptoglobin Gene Expression during Rat Liver Development and the Acute-phase Response. Mol Biol Rep 32, 141–147 (2005). https://doi.org/10.1007/s11033-005-0750-0

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