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Nucleotide sequence analysis of S-locus genes to unify S haplotype nomenclature in radish

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Abstract

Radish, belonging to the family Brassicaceae, has a self-incompatibility which is controlled by multiple alleles on the S locus. To employ the self-incompatibility in an F1 breeding system, identification of S haplotypes is necessary. Since collection of S haplotypes and determination of nucleotide sequences of SLG, SRK, and SCR alleles in cultivated radish have been conducted by different groups independently, the same or similar sequences with different S haplotype names and different sequences with the same S haplotype names have been registered in public databases, resulting in confusion of S haplotype names for researchers and breeders. In the present study, we developed S homozygous lines from radish F1 hybrid cultivars in Japan and determined the nucleotide sequences of SCR, the S domain and the kinase domain of SRK, and the SLG of a large number of S haplotypes. Comparing these sequences with our previously published sequences, the haplotypes were ordered into 23 different S haplotypes. The sequences of the 23 S haplotypes were compared with S haplotype sequences registered by different groups, and we suggested a unification of these S haplotypes. Furthermore, dot-blot hybridization using SRK allele-specific probes was examined for developing a standard method for S haplotype identification.

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Funding

This work was supported in part by JSPS KAKENHI Grant Number JP17K07599.

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Authors and Affiliations

  1. Graduate School of Agricultural Science, Tohoku University, 468-1, Aza-Aoba, Aramaki, Aoba-ku, Sendai, 980-0845, Japan

    Yurie Haseyama, Hiroyasu Kitashiba, Emiko Tonouchi & Takeshi Nishio

  2. Takii Plant Breeding & Experiment Station, Konan, Shiga, 520-3231, Japan

    Shunsuke Okamoto & Koji Sakamoto

Authors
  1. Yurie Haseyama
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  2. Hiroyasu Kitashiba
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  3. Shunsuke Okamoto
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  4. Emiko Tonouchi
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  5. Koji Sakamoto
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  6. Takeshi Nishio
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Contributions

YH, SO, and ET contributed to the implementation of the amplifications by PCR and determination of sequences. KS produced S homozygote lines. HK and TN conceived and designed the experiments. HK and TN also wrote the manuscript.

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Correspondence to Hiroyasu Kitashiba.

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Haseyama, Y., Kitashiba, H., Okamoto, S. et al. Nucleotide sequence analysis of S-locus genes to unify S haplotype nomenclature in radish. Mol Breeding 38, 116 (2018). https://doi.org/10.1007/s11032-018-0874-2

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  • DOI: https://doi.org/10.1007/s11032-018-0874-2

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