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Hybrid lethality caused by two complementary dominant genes in cabbage (Brassica oleracea L.)

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Abstract

Hybrid lethality, a type of postzygotic reproductive barrier, is important for species. Discovering novel hybrid lethality cases and analyzing corresponding causal genes may provide new insights into the establishment and maintenance of reproductive isolation. In this study, we observed the hybrid lethality phenomena in a cross between two cabbage inbred lines, 09-211 and 09-222. Genetic analysis revealed that the hybrid lethality was controlled by two complementary dominant genes, BolC.HL1.a and BolC.HL2.a, from 09-211 and 09-222, respectively. Further analysis indicated that the two genes conform to the Bateson-Dobzhansky-Muller model. Fine mapping of hybrid lethal genes revealed that BolC.HL1.a was located on the C01 chromosome by Indels HL132 and HL134, with a genetic distance of 0.2 and 0.1 cM, respectively. The interval distance between the two markers was 101 kb. BolC.HL2.a was fine-mapped on the C04 chromosome by HL235 and HL234 at a distance of 0.3 and 0.3 cM, respectively. The physical distance was 70 kb. These findings lay the foundation for cloning the hybrid lethality genes in the future and contribute to our understanding of the molecular and evolutionary mechanisms of hybrid lethality in Brassica oleracea.

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Acknowledgments

We thank Dr. Xueyuan Cao at St. Jude Children’s Research Hospital, USA, for his comments and suggestions on writing the manuscript. This work was financially supported by grants from the National Natural Science Foundation of China (31372071, 31572139), the earmarked fund for the Modern Agro-Industry Technology Research System, China (nycytx-35-gw01). This work was performed in the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Beijing 100081, People’s Republic of China.

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  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 12# ZhongGuanCun Nandajie, Beijing, 100081, People’s Republic of China

    Yang Hu, Yu-qian Xue, Ji-sheng Liu, Zhi-yuan Fang, Li-mei Yang, Yang-yong Zhang, Hong-hao Lv, Yu-mei Liu, Zhan-sheng Li & Mu Zhuang

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  1. Yang Hu
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Correspondence to Mu Zhuang.

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Hu, Y., Xue, Yq., Liu, Js. et al. Hybrid lethality caused by two complementary dominant genes in cabbage (Brassica oleracea L.). Mol Breeding 36, 73 (2016). https://doi.org/10.1007/s11032-016-0498-3

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