Skip to main content
SpringerLink
Log in

Inheritance and molecular mapping of a novel dominant genic male-sterile gene in Sesamum indicum L.

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Exploiting heterosis using pollen control systems such as genic male sterility (GMS) can significantly improve the seed yield of sesame. In this study, we developed a novel GMS line (W1098A) by backcrossing and sib-mating the wild accession Yezhi2 (Sesamum mulayanum Nair, 2n = 26) plants with the cultivar Ezhi1 (S. indicum L., 2n = 26). The sterility of this line is mainly controlled by one dominant GMS gene (Ms). Cytological investigation showed that pollen abortion in W1098A plants began in pollen mother cells, continued throughout pollen development, and peaked at the late microspore stage. The abortion process was closely associated with the abnormal behaviour of the tapetum. We employed bulked segregation analysis to identify SSR markers linked to the Ms gene in a segregating population derived from sib-mating between the near-isogenic lines W1098A and W1098B. Among 1,500 SSR primer pairs screened against the sterile and fertile bulks of DNA, 13 markers were found to be linked to the Ms locus. The closest markers on both sides of Ms were SBM298 and GB50, at a respective genetic distance of 0.15 and 0.70 cM. These markers will be valuable for marker-aided breeding of GMS hybrids and map-based cloning of Ms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Anitha VY, Ganesan J (2000) Development of male sterility system in sesame (Sesamum indicum L.). Sesame Safflower Newsl 15:20–25

  • Bhayan J, Sarma MK (2003) Identification of heterotic crosses involving cytoplasmic genic male sterile lines in sesame (Sesamum indicum L.). Sesame Safflower Newsl 18:7–11

    Google Scholar 

  • Biswas AK, Mitra AK (1999) Inter-specific hybridization in three species of sesamum. Indian J Genet Plant Breed 50:307–309

    Google Scholar 

  • Budowski P, Markley KS (1951) The chemical and physiological properties of sesame oil. Chem Rev 48:125–151

    Article  CAS  PubMed  Google Scholar 

  • Coulman KD, Liu Z, Hum WQ, Michaelides J, Thompson LU (2005) Whole sesame seed is as rich a source of mammalian lignan precursors as whole flaxseed. Nutr Cancer 52:156–165

    Article  CAS  PubMed  Google Scholar 

  • Dabral KC, Mandloi KC (1974) A peculiar sterility in sesame (Sesamum indicum L.). JNKVV Res J Jabalpur 8:54–56

    Google Scholar 

  • Dixit A, Jin MH, Chung JW, Yu JW, Chung HK, Yu JW, Chung HK, Ma KH, Park YJ, Cho EG (2005) Development of polymorphic microsatellite markers in sesame (Sesamum indicum L.). Mol Ecol Notes 5:736–738

    Article  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Erbas M, Sekerci H, Gul S, Furat S, Yol E, Uzon B (2009) Changes in total antioxidant capacity of sesame (Sesamum indicum L.) by variety. Asian J Chem 21:5549–5555

    CAS  Google Scholar 

  • Fu TD, Yang GS, Yang XN (1990) Studies on ‘three line’ Polima cytoplasmic male sterility developed in Brassica napus L. Plant Breed 104:115–120

    Article  Google Scholar 

  • Gao HS, Liu JR, Tu LC (1992) Cytological studies on the mechanism of abortion of microsporogenesis in nucleic male sterile (NMS) sesame (Sesamum indicumL.). Acta Agron Sin 18:425–428

    Google Scholar 

  • Guo W, Miao HM, Zhang TD, Wei LB, Li C, Zhang HY (2014) Cytological observation of partial sterility of nucleic male sterile line ms86-1 in sesame (Sesamum indicum). Chin Bull Bot 49:87–97

    Google Scholar 

  • Karatzi K, Stamatelopoulos K, Lykka M, Mantzouratou P, Skalidi S, Zakopoulos N (2013) Sesame oil consumption exerts a beneficial effect on endothelial function in hypertensive men. Eur J Prev Cardiol 20:202–208

    Article  PubMed  Google Scholar 

  • Kavitha M, Ramalingam RS, Raveendran TS, Punitha D (2000) Cytoplasmic-genic male sterile lines in sesame: Effect of environmental factors. Crop Res 19:162–164

    Google Scholar 

  • Kumar LS, Abraham A (1941) A cytological study of sterility in sesame. Indian J Genet Plant Breed 1:41–60

    Google Scholar 

  • Kumar LS, Rao DS (1945) Inheritance of sterility in Sesamum indicum L. Indian J Genet Plant Breed 1:58–59

    Google Scholar 

  • Li YD, Chen QM (1998) Studies on induced mutation and preliminary genetics of male sterile mutants in sesame (Sesamum indicum L.). Chin J Oil Crop Sci 20:24–27

    CAS  Google Scholar 

  • Li SL, Qian YX, Wu ZH (1985) Genetic study on genic male sterility and its utilization in Brassica napus L. Acta Agric Shanghai 1:1–12

    Google Scholar 

  • Li SL, Zhou ZJ, Zhou XR (1995) Three-line method of genetic male sterility for hybrid seed production in Brassica napus L. Acta Agric Shanghai 11:21–26

    Google Scholar 

  • Li SG, Zhou KD, Zhu LH (1999) Molecular mapping and genetic analysis of dominant themo-sensitive genic male sterile in rice. Chin Sci Bull 44:955–958

    Google Scholar 

  • Li SQ, Yang DC, Zhu YG (2007) Characterization and use of male sterility in hybrid rice breeding. J Integr Plant Biol 49:791–804

    Article  CAS  Google Scholar 

  • Lin ZX, He DH, Zhang XL, Nie YC, Guo XP, Feng CD, Stewart JM (2005) Linkage map construction and mapping QTL for cotton fiber quality using SRAP, SSR and RAPD. Plant Breed 124:180–187

    Article  CAS  Google Scholar 

  • Liu HY, Yang MM, Wu K, Zhou XA, Zhao YZ (2013) Development, inheritance and breeding potential of a recessive genic male sterile line D248A in Sesame (Sesamum indicum L.). SpringerPlus 2:268–276

    Article  PubMed Central  PubMed  Google Scholar 

  • Lu GY, Zhang XK, Li GY, Chen Y, Zheng PY (2012) Breeding of high yield, double low and disease resistant rapeseed cultivars by population improvement. Chin J Oil Crops Sci 34:575–581

    Google Scholar 

  • Ma H (2005) Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants. Annu Rev Plant Biol 56:393–434

    Article  CAS  PubMed  Google Scholar 

  • Ma JH, Wei HL, Liu J, Song MZ, Pang CY, Wang L, Zhang WX, Fan SL, Yu SX (2013) Selection and characterization of a novel photoperiod-sensitive male sterile line in upland cotton. J Integr Plant Biol 55:608–618

    Article  CAS  PubMed  Google Scholar 

  • Malaguti G, Mazzani B (1958) Cases of sterility in sesame. Agron Trop Venezuela 8:63–65

    Google Scholar 

  • Mathias R (1985) A new dominant gene for male sterility in rapeseed, Brassica napus L. Z Pflanzenzuchtung 94:170–173

    Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Osman HE, Yermanos DM (1982) Genetic male sterility in sesame: reproductive characteristics and possible use in hybrid seed production. Crop Sci 22:492–498

    Article  Google Scholar 

  • Pal BP (1945) Studies in hybrid vigor of sesame (Sesamum indicum L.). Indian J Genet Plant Breed 5:106–121

    Google Scholar 

  • Periasamy S, Hsu DZ, Chang PC, Liu MY (2014) Sesame oil attenuates nutritional fibrosingsteatohepatitis by modulating matrix metalloproteinases-2, 9 and PPAR-gamma. J Nutr Biochem 25:337–344

    Article  CAS  PubMed  Google Scholar 

  • Prabakaran AJ (1998) Cytoplasmic male sterility in sesame from the species cross, Sesameum malabaircum × S. indicum: II. Microsporogensis and outcrossing. In: Martínez JF (ed) Sesame safflower Newsl. CSIC Press, Córdoba, pp 7–12

    Google Scholar 

  • Shi YL, Zhao S, Yao JL (2009) Premature tapetum degeneration: a major cause of abortive pollen development in photoperiod sensitive genic male sterility in rice. J Integr Plant Biol 51:774–781

    Article  PubMed  Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package, join map. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Taylor PE, Glover JA, Lavithis M, Craig S, Singh MB, Knot RB, Dennis ES, Chaudhury AM (1998) Genetic control of male fertility in Arabidopsis thaliana: structural analyses of postmeiotic developmental mutants. Planta 205:492–505

    Article  CAS  PubMed  Google Scholar 

  • Tu LC, Liang XY, Wang WQ, Zheng YZ, Liu JR (1995) Studies on the genetic male sterile in sesame. Acta Agric Boreali-Sin 10:34–39

    Google Scholar 

  • Uzun B, Lee D, Donini P, Cagirgan MI (2003) Identification of a molecular marker linked to the closed capsule mutant trait in sesame using AFLP. Plant Breed 122:95–97

    Article  CAS  Google Scholar 

  • Uzun B, Ozbas MO, Canci H, Cagirgan MI (2004) Heterosis for agronomic traits in sesame hybrids of cultivars × closed capsule mutants. Acta Agr Scand B-S P 54:108–112

    Google Scholar 

  • Uzun B, Arslan Ç, Furat S (2008) Variation in fatty acid compositions, oil content and oil yield in a germplasm collection of sesame (Sesamum indicum L.). J Am Oil Chem Soc 85:1135–1142

    Article  CAS  Google Scholar 

  • Wang Q, Wang BC (2002) Study on the mechanism of sesame male sterile and its hereditary. J Anhui Agric Sci 30:837–838

    Google Scholar 

  • Wang DJ, Guo AG, Li DR, Tian JH, Huang F, Sun GL (2007) Cytological and molecular characterization of a novel monogenic dominant GMS in Brassica napus L. Plant Cell Rep 26:571–579

    Article  PubMed  Google Scholar 

  • Wang LH, Yu S, Tong CB, Zhao YZ, Liu Y, Song C, Zhang YX, Zhang XD, Wang Y, Hua W, Li DH, Li D, Li F, Yu JY, Xu CY, Han XL, Huang SM, Tai SS, Wang JY, Xu X, Li YR, Liu SY, Varshney RK, Wang J, Zhang XR (2014) Genome sequencing of the high oil crops sesame provides insight into oil biosynthesis. Genome Biol. doi:10.1186/gb-2014-15-2-r39

    Google Scholar 

  • Wanjari KB, Patil AN, Patel MC, Manjaya JG (2000) Male sterility derived from Cajanussericeus × Cajanuscajan: confusion of cytoplasmic male-sterility with dominant genic male-sterility. Euphytica 115:59–64

    Article  Google Scholar 

  • Wei LB, Zhang HY, Zheng YZ, Miao HM, Zhang TZ, Guo WZ (2009) A genetic linkage map construction for sesame (Sesamum indicum L.). Genes Genom 31:199–208

    Article  CAS  Google Scholar 

  • Yang M, Huang FH (2009) Situation, problem, development trend and suggestion of sesame industry in China. Chin Oils Fats 34:7–12

    CAS  Google Scholar 

  • Yang GS, Fu TD, Ma CZ, Yang XN (1996) Establishment of a random mating population of Polima cytoplasmic male sterility restorers in Brasica napus L. J Huazhong Agric Univ 15:316–321

    Google Scholar 

  • Yang XL, Zhang HY, Guo WZ, Zheng YZ, Miao HM, Wei LB, Zhang TZ (2008) Ultrastructure in microspore abortion of genic male sterile line in sesame (Sesamum indicum L.). Acta Agron Sin 34:1894–1900

    Article  Google Scholar 

  • Zhang TD, Zhang HY, Zheng YZ, Wei SL, Mei HX (2005) A review of heterosis and seed production techniques in sesame. Crops 5:64–67

    Google Scholar 

  • Zhang HY, Wei LB, Miao HM, Zhang TD, Wang CY (2012) Development and validation of genic-SSR markers in sesame by RNA-seq. BMC Genom 13:316–326

    Article  CAS  Google Scholar 

  • Zhang HY, Miao HM, Wei LB, Li C, Zhao RH, Wang CY (2013) Genetic analysis and QTL mapping of seed coat color in sesame (Sesamum indicum L.). PLoS One 8:e63898

  • Zhang YX, Wang LH, Xin HG, Li DH, Ma CX, Ding X, Hong WG, Zhang XR (2013) Construction of a high-density genetic map for sesame based on large scale marker development by specific length amplified fragment (SLAF) sequencing. BMC Plant Biol 13:141–152

  • Zhao YZ, Liu HY (2008) The breeding and key cultivation points of hybrid sesame variety Zhongzhiza No.1. World Agric 11:147–150

    Google Scholar 

  • Zhao YZ, Yang MM, Wu K, Liu HY, Wu JS, Liu KD (2013) Characterization and genetic mapping of a novel recessive genic male sterile gene in sesame (Sesamum indicum L.). Mol Breed 32:901–908

    Article  CAS  Google Scholar 

  • Zheng YZ, Zhang HY, Mei HX, Zhang TD, Wei SL (2003) Advances in Chinese hybrid sesame research. J Henan Agric Sci 11:17–19

    Google Scholar 

Download references

Acknowledgments

This research was funded by National Natural Science Foundation of China (31101180) and China’s National Agricultural Research System (CARS-15).

Author information

Authors and Affiliations

  1. Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS, Wuhan, 430062, China

    Hongyan Liu, Xinan Zhou, Kun Wu, Minmin Yang & Yingzhong Zhao

Authors
  1. Hongyan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xinan Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kun Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Minmin Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yingzhong Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yingzhong Zhao.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, H., Zhou, X., Wu, K. et al. Inheritance and molecular mapping of a novel dominant genic male-sterile gene in Sesamum indicum L.. Mol Breeding 35, 9 (2015). https://doi.org/10.1007/s11032-015-0189-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11032-015-0189-5

Keywords

Search

Navigation