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Development of 240 novel EST-SSRs in Eucalyptus L’Hérit

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Abstract

Simple sequence repeat (SSR) markers derived from expressed sequence tag (EST) resources provide great potential for comparative mapping, direct gene tagging of quantitative trait loci and functional diversity studies. Here we report on the development of 240 novel EST-SSRs for the important tree genus Eucalyptus L’Hérit. Of the 240 EST-SSR loci, 218 (90.8 %) were polymorphic among 12 individuals of E. grandis Hill ex Maiden, with the number of alleles per locus (N a), observed heterozygosity (H o), expected heterozygosity (H e) and polymorphic information content (PIC) averaging at 5.0, 0.403, 0.598 and 0.529, respectively. High rates of cross-species/subgenus amplification were observed. The EST-SSRs developed herein would be a valuable addition of functional markers for genetics and breeding applications in a wide range of eucalypt species. The primer sequences for the 240 EST-SSRs have been deposited in the Probe database of GenBank (IDs Pr016588534–773).

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Acknowledgments

This work was financially supported by grants from the Ministry of Science and Technology of China (2013AA100705, 2012CB114505 and 2011AA100202) and the National Natural Science Foundation of China (31070592). The authors are grateful to Kunming Wu and Juying Wu for plant material maintenance.

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Road, Beijing, 100091, China

    Changpin Zhou & Siming Gan

  2. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Longdong, Guangzhou, 510520, China

    Changpin Zhou, Fagen Li, Qijie Weng, Xiaoli Yu, Yu Wang, Mei Li & Siming Gan

  3. Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, 159 Longpan Road, Nanjing, 210037, China

    Xudong He & Jisen Shi

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  1. Changpin Zhou
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  2. Xudong He
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  3. Fagen Li
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  4. Qijie Weng
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  5. Xiaoli Yu
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  6. Yu Wang
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  7. Mei Li
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  8. Jisen Shi
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  9. Siming Gan
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Correspondence to Jisen Shi or Siming Gan.

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Zhou, C., He, X., Li, F. et al. Development of 240 novel EST-SSRs in Eucalyptus L’Hérit. Mol Breeding 33, 221–225 (2014). https://doi.org/10.1007/s11032-013-9923-z

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